EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.7 | additional information | - |
additional information | steady-state kinetic parameters for next base extension from G:C and AP site:A (or C) template:primer termini | Thermococcus litoralis | |
2.7.7.7 | additional information | - |
additional information | steady-state kinetic parameters for next base extension from G:C and AP site:A (or C) template:primer termini | Saccharolobus solfataricus | |
2.7.7.7 | 0.0041 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Saccharolobus solfataricus | |
2.7.7.7 | 0.0062 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Thermococcus litoralis | |
2.7.7.7 | 0.012 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Saccharolobus solfataricus | |
2.7.7.7 | 0.012 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Saccharolobus solfataricus | |
2.7.7.7 | 0.026 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Saccharolobus solfataricus | |
2.7.7.7 | 0.027 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Saccharolobus solfataricus | |
2.7.7.7 | 0.045 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Saccharolobus solfataricus | |
2.7.7.7 | 0.05 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Saccharolobus solfataricus | |
2.7.7.7 | 0.052 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Saccharolobus solfataricus | |
2.7.7.7 | 0.077 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Saccharolobus solfataricus | |
2.7.7.7 | 0.084 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Thermococcus litoralis | |
2.7.7.7 | 0.11 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Saccharolobus solfataricus | |
2.7.7.7 | 0.13 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Thermococcus litoralis | |
2.7.7.7 | 0.25 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Saccharolobus solfataricus | |
2.7.7.7 | 0.31 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Thermococcus litoralis | |
2.7.7.7 | 0.38 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Thermococcus litoralis | |
2.7.7.7 | 0.43 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Saccharolobus solfataricus | |
2.7.7.7 | 0.43 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Thermococcus litoralis | |
2.7.7.7 | 0.59 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Saccharolobus solfataricus | |
2.7.7.7 | 0.6 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Saccharolobus solfataricus | |
2.7.7.7 | 0.79 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Saccharolobus solfataricus | |
2.7.7.7 | 0.83 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Thermococcus litoralis | |
2.7.7.7 | 0.87 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Saccharolobus solfataricus | |
2.7.7.7 | 0.88 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Saccharolobus solfataricus | |
2.7.7.7 | 0.94 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Saccharolobus solfataricus | |
2.7.7.7 | 0.96 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Thermococcus litoralis | |
2.7.7.7 | 1.1 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Saccharolobus solfataricus | |
2.7.7.7 | 1.2 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Thermococcus litoralis | |
2.7.7.7 | 1.2 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Saccharolobus solfataricus | |
2.7.7.7 | 1.2 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Thermococcus litoralis | |
2.7.7.7 | 1.3 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Saccharolobus solfataricus | |
2.7.7.7 | 1.3 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Thermococcus litoralis | |
2.7.7.7 | 1.5 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Thermococcus litoralis | |
2.7.7.7 | 1.6 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Thermococcus litoralis | |
2.7.7.7 | 1.8 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Thermococcus litoralis | |
2.7.7.7 | 2.1 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Thermococcus litoralis | |
2.7.7.7 | 2.2 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Saccharolobus solfataricus | |
2.7.7.7 | 2.3 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Thermococcus litoralis | |
2.7.7.7 | 2.3 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Thermococcus litoralis | |
2.7.7.7 | 2.3 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Thermococcus litoralis | |
2.7.7.7 | 2.3 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Thermococcus litoralis | |
2.7.7.7 | 2.4 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Thermococcus litoralis | |
2.7.7.7 | 2.5 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Thermococcus litoralis | |
2.7.7.7 | 2.7 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Thermococcus litoralis | |
2.7.7.7 | 2.8 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Thermococcus litoralis | |
2.7.7.7 | 3.2 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Thermococcus litoralis | |
2.7.7.7 | 3.8 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Saccharolobus solfataricus | |
2.7.7.7 | 8.9 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Saccharolobus solfataricus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.7 | Saccharolobus solfataricus | Q97W02 | - |
- |
2.7.7.7 | Saccharolobus solfataricus P2 | Q97W02 | - |
- |
2.7.7.7 | Thermococcus litoralis | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.7 | dATP + DNAn | - |
Thermococcus litoralis | ? | - |
? | |
2.7.7.7 | dATP + DNAn | - |
Saccharolobus solfataricus | ? | - |
? | |
2.7.7.7 | dATP + DNAn | - |
Saccharolobus solfataricus P2 | ? | - |
? | |
2.7.7.7 | dCTP + DNAn | - |
Thermococcus litoralis | ? | - |
? | |
2.7.7.7 | dCTP + DNAn | - |
Saccharolobus solfataricus | ? | - |
? | |
2.7.7.7 | dCTP + DNAn | - |
Saccharolobus solfataricus P2 | ? | - |
? | |
2.7.7.7 | deoxynucleoside triphosphate + DNAn | the enzyme readily bypasses N2-methyl(Me)G and O6-MeG, but is strongly blocked at O6-benzyl(Bz)G and N2-BzG. the enzyme shows 110-, 180-, and 300fold decreases in catalytic efficiency (kcat/Km) for nucleotide insertion opposite an abasicP site, N2-MeG, and O6-MeG but 1800- and 5000fold decreases opposite O6-BzG and N2-BzG, respectively, as compared to G. DNA polymerase Vent (exo-) from Thermococcus litoralis is as or more efficient as polymerase Dpo4 from Sulfolobus solfataricus in synthesis opposite O6-MeG and AP lesions, whereas DNA polymerase Dpo4 from Sulfolobus solfataricus is much or more efficient opposite N2-alkylGs than DNA polymerase Vent (exo-) from Thermococcus litoralis, irrespective of DNA-binding affinity. DNA polymerase Vent (exo-) accepts nonbulky DNA lesions (e.g., N2- or O6-MeG and an AP site) as manageable substrates despite causing errorprone synthesis | Thermococcus litoralis | diphosphate + DNAn+1 | - |
? | |
2.7.7.7 | deoxynucleoside triphosphate + DNAn | the enzyme shows little decreases opposite N2-MeG, 13fold decrease opposite N2-BzG but 260-370fold decreases opposite O6-MeG, O6-BzG, and the abasic site site as compared to G. Dpo4 favored correct C insertion opposite opposite N2-MeG, opposite O6-MeG, opposite an abasic site site and oppositeN2-BzG. DNA polymerase Vent (exo-) from Thermococcus litoralis is as or more efficient as polymerase Dpo4 from Sulfolobus solfataricus in synthesis opposite O6-MeG and AP lesions, whereas DNA polymerase Dpo4 from Sulfolobus solfataricus is much or more efficient opposite N2-alkylGs than DNA polymerase Vent (exo-) from Thermococcus litoralis, irrespective of DNA-binding affinity. DNA polymerase Dpo4 strongly favors minor-groove N2-alkylG lesions over major-groove or noninstructive lesions | Saccharolobus solfataricus | diphosphate + DNAn+1 | - |
? | |
2.7.7.7 | deoxynucleoside triphosphate + DNAn | the enzyme shows little decreases opposite N2-MeG, 13fold decrease opposite N2-BzG but 260-370fold decreases opposite O6-MeG, O6-BzG, and the abasic site site as compared to G. Dpo4 favored correct C insertion opposite opposite N2-MeG, opposite O6-MeG, opposite an abasic site site and oppositeN2-BzG. DNA polymerase Vent (exo-) from Thermococcus litoralis is as or more efficient as polymerase Dpo4 from Sulfolobus solfataricus in synthesis opposite O6-MeG and AP lesions, whereas DNA polymerase Dpo4 from Sulfolobus solfataricus is much or more efficient opposite N2-alkylGs than DNA polymerase Vent (exo-) from Thermococcus litoralis, irrespective of DNA-binding affinity. DNA polymerase Dpo4 strongly favors minor-groove N2-alkylG lesions over major-groove or noninstructive lesions | Saccharolobus solfataricus P2 | diphosphate + DNAn+1 | - |
? | |
2.7.7.7 | dGTP + DNAn | - |
Thermococcus litoralis | ? | - |
? | |
2.7.7.7 | dGTP + DNAn | - |
Saccharolobus solfataricus | ? | - |
? | |
2.7.7.7 | dGTP + DNAn | - |
Saccharolobus solfataricus P2 | ? | - |
? | |
2.7.7.7 | dTTP + DNAn | - |
Thermococcus litoralis | ? | - |
? | |
2.7.7.7 | dTTP + DNAn | - |
Saccharolobus solfataricus | ? | - |
? | |
2.7.7.7 | dTTP + DNAn | - |
Saccharolobus solfataricus P2 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.7.7 | Dpo4 | - |
Saccharolobus solfataricus |
2.7.7.7 | pol Vent (exo-) | - |
Thermococcus litoralis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.7 | additional information | - |
additional information | steady-state kinetic parameters for next base extension from G:C and AP site:A (or C) template:primer termini | Thermococcus litoralis | |
2.7.7.7 | additional information | - |
additional information | steady-state kinetic parameters for next base extension from G:C and AP site:A (or C) template:primer termini | Saccharolobus solfataricus | |
2.7.7.7 | 0.0001 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Thermococcus litoralis | |
2.7.7.7 | 0.00019 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Saccharolobus solfataricus | |
2.7.7.7 | 0.0002 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Thermococcus litoralis | |
2.7.7.7 | 0.00032 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Saccharolobus solfataricus | |
2.7.7.7 | 0.0004 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Thermococcus litoralis | |
2.7.7.7 | 0.00055 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Saccharolobus solfataricus | |
2.7.7.7 | 0.0006 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Thermococcus litoralis | |
2.7.7.7 | 0.0008 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Thermococcus litoralis | |
2.7.7.7 | 0.0012 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Saccharolobus solfataricus | |
2.7.7.7 | 0.0016 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Saccharolobus solfataricus | |
2.7.7.7 | 0.0016 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Saccharolobus solfataricus | |
2.7.7.7 | 0.0017 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Thermococcus litoralis | |
2.7.7.7 | 0.0021 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Thermococcus litoralis | |
2.7.7.7 | 0.0021 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Thermococcus litoralis | |
2.7.7.7 | 0.0022 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Saccharolobus solfataricus | |
2.7.7.7 | 0.0023 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Saccharolobus solfataricus | |
2.7.7.7 | 0.0027 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Saccharolobus solfataricus | |
2.7.7.7 | 0.0028 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Saccharolobus solfataricus | |
2.7.7.7 | 0.0029 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Thermococcus litoralis | |
2.7.7.7 | 0.003 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Thermococcus litoralis | |
2.7.7.7 | 0.0033 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Saccharolobus solfataricus | |
2.7.7.7 | 0.0036 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Saccharolobus solfataricus | |
2.7.7.7 | 0.0045 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Saccharolobus solfataricus | |
2.7.7.7 | 0.0049 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Saccharolobus solfataricus | |
2.7.7.7 | 0.0052 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Thermococcus litoralis | |
2.7.7.7 | 0.0053 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Thermococcus litoralis | |
2.7.7.7 | 0.0055 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Saccharolobus solfataricus | |
2.7.7.7 | 0.0057 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Saccharolobus solfataricus | |
2.7.7.7 | 0.0062 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Saccharolobus solfataricus | |
2.7.7.7 | 0.0067 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Thermococcus litoralis | |
2.7.7.7 | 0.0068 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Thermococcus litoralis | |
2.7.7.7 | 0.0098 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Thermococcus litoralis | |
2.7.7.7 | 0.012 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Thermococcus litoralis | |
2.7.7.7 | 0.014 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Thermococcus litoralis | |
2.7.7.7 | 0.017 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Thermococcus litoralis | |
2.7.7.7 | 0.021 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Saccharolobus solfataricus | |
2.7.7.7 | 0.021 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Saccharolobus solfataricus | |
2.7.7.7 | 0.022 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Thermococcus litoralis | |
2.7.7.7 | 0.025 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Saccharolobus solfataricus | |
2.7.7.7 | 0.025 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Saccharolobus solfataricus | |
2.7.7.7 | 0.027 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Thermococcus litoralis | |
2.7.7.7 | 0.043 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Thermococcus litoralis | |
2.7.7.7 | 0.043 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Thermococcus litoralis | |
2.7.7.7 | 0.043 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Thermococcus litoralis | |
2.7.7.7 | 0.063 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Saccharolobus solfataricus | |
2.7.7.7 | 0.065 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Thermococcus litoralis | |
2.7.7.7 | 0.092 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Saccharolobus solfataricus | |
2.7.7.7 | 0.38 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Saccharolobus solfataricus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.7 | additional information | - |
additional information | steady-state kinetic parameters for next base extension from G:C and AP site:A (or C) template:primer termini | Thermococcus litoralis | |
2.7.7.7 | additional information | - |
additional information | steady-state kinetic parameters for next base extension from G:C and AP site:A (or C) template:primer termini | Saccharolobus solfataricus | |
2.7.7.7 | 0.000042 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Thermococcus litoralis | |
2.7.7.7 | 0.00017 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Thermococcus litoralis | |
2.7.7.7 | 0.0004 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Thermococcus litoralis | |
2.7.7.7 | 0.00064 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Thermococcus litoralis | |
2.7.7.7 | 0.001 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Saccharolobus solfataricus | |
2.7.7.7 | 0.0011 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Thermococcus litoralis | |
2.7.7.7 | 0.0011 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Thermococcus litoralis | |
2.7.7.7 | 0.0012 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Thermococcus litoralis | |
2.7.7.7 | 0.0016 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Saccharolobus solfataricus | |
2.7.7.7 | 0.0016 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Saccharolobus solfataricus | |
2.7.7.7 | 0.0021 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Thermococcus litoralis | |
2.7.7.7 | 0.0022 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Thermococcus litoralis | |
2.7.7.7 | 0.0025 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Thermococcus litoralis | |
2.7.7.7 | 0.0026 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Saccharolobus solfataricus | |
2.7.7.7 | 0.0026 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Saccharolobus solfataricus | |
2.7.7.7 | 0.0028 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Saccharolobus solfataricus | |
2.7.7.7 | 0.0029 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Thermococcus litoralis | |
2.7.7.7 | 0.0031 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Thermococcus litoralis | |
2.7.7.7 | 0.0032 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Saccharolobus solfataricus | |
2.7.7.7 | 0.0034 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Saccharolobus solfataricus | |
2.7.7.7 | 0.0043 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Thermococcus litoralis | |
2.7.7.7 | 0.0055 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Saccharolobus solfataricus | |
2.7.7.7 | 0.0061 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Thermococcus litoralis | |
2.7.7.7 | 0.0065 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Saccharolobus solfataricus | |
2.7.7.7 | 0.0081 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Thermococcus litoralis | |
2.7.7.7 | 0.01 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Thermococcus litoralis | |
2.7.7.7 | 0.01 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Saccharolobus solfataricus | |
2.7.7.7 | 0.016 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Thermococcus litoralis | |
2.7.7.7 | 0.017 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Saccharolobus solfataricus | |
2.7.7.7 | 0.019 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Saccharolobus solfataricus | |
2.7.7.7 | 0.019 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Saccharolobus solfataricus | |
2.7.7.7 | 0.019 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Thermococcus litoralis | |
2.7.7.7 | 0.019 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Thermococcus litoralis | |
2.7.7.7 | 0.02 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Saccharolobus solfataricus | |
2.7.7.7 | 0.021 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Thermococcus litoralis | |
2.7.7.7 | 0.022 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Saccharolobus solfataricus | |
2.7.7.7 | 0.027 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Saccharolobus solfataricus | |
2.7.7.7 | 0.03 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Saccharolobus solfataricus | |
2.7.7.7 | 0.037 | - |
dTTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Thermococcus litoralis | |
2.7.7.7 | 0.062 | - |
dGTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Thermococcus litoralis | |
2.7.7.7 | 0.083 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Saccharolobus solfataricus | |
2.7.7.7 | 0.099 | - |
dATP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template: abasic site | Thermococcus litoralis | |
2.7.7.7 | 0.11 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-BzG | Saccharolobus solfataricus | |
2.7.7.7 | 0.12 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: O6-MeG | Saccharolobus solfataricus | |
2.7.7.7 | 2.4 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-BzG | Saccharolobus solfataricus | |
2.7.7.7 | 11 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Thermococcus litoralis | |
2.7.7.7 | 23 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: N2-MeG | Saccharolobus solfataricus | |
2.7.7.7 | 31 | - |
dCTP | pH 7.5, 37°C, dNTP incorporation opposite DNA lesions, template base: G | Saccharolobus solfataricus |