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Literature summary extracted from

  • Runguphan, W.; Glenn, W.S.; OConnor, S.E.
    Redesign of a dioxygenase in morphine biosynthesis (2012), Chem. Biol., 19, 674-678.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.32 ascorbate
-
 Papaver somniferum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.32 expression of His-tagged wild-type enzyme and mutants in Escherichia coli strain SG13009 Papaver somniferum

Protein Variants

EC Number Protein Variants Comment Organism
1.14.11.32 E338G/I340L/L341V/K342E site-directed mutagenesis, the mutant exhibits demethylase activity exclusively toward codeine, not also toward thebaine like the wild-type enzyme Papaver somniferum
1.14.11.32 R334S/R336S site-directed mutagenesis, the mutant retains O-demethylase activity toward either thebaine or codeine like the wild-type enzyme Papaver somniferum
1.14.11.32 R334S/R336T site-directed mutagenesis, the mutant retains O-demethylase activity toward either thebaine or codeine like the wild-type enzyme Papaver somniferum
1.14.11.32 S149M/L151F/S152T site-directed mutagenesis, the mutant is expressed at low levels, perhaps due to improper folding Papaver somniferum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.32 0.0728
-
 codeine pH 7.4, 30°C, wild-type enzyme Papaver somniferum
1.14.11.32 0.216
-
 thebaine pH 7.4, 30°C, wild-type enzyme Papaver somniferum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.32 Fe2+ required Papaver somniferum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.32 codeine + 2-oxoglutarate + O2 Papaver somniferum
-
 morphine + formaldehyde + succinate + CO2
-
 ?
1.14.11.32 thebaine + 2-oxoglutarate + O2 Papaver somniferum
-
 oripavine + formaldehyde + succinate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.32 Papaver somniferum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.32 recombinant His-tagged wild-type enzyme and mutants from Escherichia coli strain SG13009 by cobalt affinity chromatography Papaver somniferum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.32 codeine + 2-oxoglutarate + O2
-
 Papaver somniferum morphine + formaldehyde + succinate + CO2
-
 ?
1.14.11.32 thebaine + 2-oxoglutarate + O2
-
 Papaver somniferum oripavine + formaldehyde + succinate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.32 CODM
-
 Papaver somniferum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.32 30
-
 assay at Papaver somniferum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.32 7.4
-
 assay at Papaver somniferum

General Information

EC Number General Information Comment Organism
1.14.11.32 metabolism the enzyme is involved in the morphinan pathway via codeine of the biosynthesis of benzylisoquinoline alkaloids in Papaver somniferum, overview Papaver somniferum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.11.32 0.000015
-
 thebaine pH 7.4, 30°C, wild-type enzyme Papaver somniferum
1.14.11.32 0.00095
-
 codeine pH 7.4, 30°C, wild-type enzyme Papaver somniferum