EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.326 | AKnT protein | the enzyme AknS has very low activity in the absence of the adjacently encoded AknT activator protein. At a 3:1 ratio in the 0.0025-0.01 mM range, AknT stimulates AknS kcat by 40fold for transfer of L-2-deoxyfucose to aklavinone | Streptomyces galilaeus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.326 | expressed in Escherichia coli BL21(DE3) cells | Streptomyces galilaeus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.326 | 0.0031 | - |
aklavinone | with dTDP-4-amino-2-deoxyrhamnose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C | Streptomyces galilaeus | |
2.4.1.326 | 0.0035 | - |
aklavinone | with dTDP-L-2-deoxyfucose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C | Streptomyces galilaeus | |
2.4.1.326 | 0.046 | - |
epsilon-rhodomycinone | with dTDP-L-2-deoxyfucose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C | Streptomyces galilaeus | |
2.4.1.326 | 0.156 | - |
dTDP-L-2-deoxyfucose | with epsilon-rhodomycinone as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C | Streptomyces galilaeus | |
2.4.1.326 | 0.2878 | - |
dTDP-L-2-deoxyfucose | with aklavinone as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C | Streptomyces galilaeus | |
2.4.1.326 | 0.4072 | - |
dTDP-4-amino-2-deoxyrhamnose | in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C | Streptomyces galilaeus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.326 | dTDP-beta-L-rhodosamine + aklavinone | Streptomyces galilaeus | - |
dTDP + aclacinomycin T | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.326 | Streptomyces galilaeus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.326 | Ni-NTA affinity column chromatography, and gel filtration | Streptomyces galilaeus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.326 | dTDP-4-amino-2-deoxyrhamnose + aklavinone | - |
Streptomyces galilaeus | dTDP + 4-O-(2,4-dideoxy-4-amino-alpha-L-rhamnopyranosyl)aklavinone | - |
? | |
2.4.1.326 | dTDP-4-amino-2-deoxyrhamnose + aklavinone | - |
Streptomyces galilaeus | dTDP + 4-O-(2,4-dideoxy-4-amino-alpha-L-rhamnosyl)aklavinone | - |
? | |
2.4.1.326 | dTDP-beta-L-rhodosamine + aklavinone | - |
Streptomyces galilaeus | dTDP + aclacinomycin T | - |
? | |
2.4.1.326 | dTDP-L-2-deoxyfucose + aklavinone | - |
Streptomyces galilaeus | dTDP + 4-O-(2-deoxy-alpha-L-fucosyl)aklavinone | - |
? | |
2.4.1.326 | dTDP-L-2-deoxyfucose + epsilon-rhodomycinone | - |
Streptomyces galilaeus | dTDP + 4-O-(2-deoxy-alpha-L-fucosyl)-epsilon-rhodomycinone | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.326 | AknS | - |
Streptomyces galilaeus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.326 | 0.001 | - |
epsilon-rhodomycinone | with dTDP-L-2-deoxyfucose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C | Streptomyces galilaeus | |
2.4.1.326 | 0.001 | - |
dTDP-L-2-deoxyfucose | with epsilon-rhodomycinone as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C | Streptomyces galilaeus | |
2.4.1.326 | 0.003 | - |
dTDP-4-amino-2-deoxyrhamnose | in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C | Streptomyces galilaeus | |
2.4.1.326 | 0.003 | - |
aklavinone | with dTDP-4-amino-2-deoxyrhamnose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C | Streptomyces galilaeus | |
2.4.1.326 | 0.004 | - |
dTDP-L-2-deoxyfucose | with aklavinone as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C | Streptomyces galilaeus | |
2.4.1.326 | 0.004 | - |
aklavinone | with dTDP-L-2-deoxyfucose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C | Streptomyces galilaeus |