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Literature summary extracted from

  • Lu, W.; Leimkuhler, C.; Gatto, G.J. Jr.
    Kruger, R.G.; Oberthür, M.; Kahne, D.; Walsh, C.T.: AknT is an activating protein for the glycosyltransferase AknS in L-aminodeoxysugar transfer to the aglycone of aclacinomycin A (2005), Chem. Biol., 12, 527-534.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.326 AKnT protein the enzyme AknS has very low activity in the absence of the adjacently encoded AknT activator protein. At a 3:1 ratio in the 0.0025-0.01 mM range, AknT stimulates AknS kcat by 40fold for transfer of L-2-deoxyfucose to aklavinone Streptomyces galilaeus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.326 expressed in Escherichia coli BL21(DE3) cells Streptomyces galilaeus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.326 0.0031
-
 aklavinone with dTDP-4-amino-2-deoxyrhamnose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C Streptomyces galilaeus
2.4.1.326 0.0035
-
 aklavinone with dTDP-L-2-deoxyfucose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C Streptomyces galilaeus
2.4.1.326 0.046
-
 epsilon-rhodomycinone with dTDP-L-2-deoxyfucose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C Streptomyces galilaeus
2.4.1.326 0.156
-
 dTDP-L-2-deoxyfucose with epsilon-rhodomycinone as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C Streptomyces galilaeus
2.4.1.326 0.2878
-
 dTDP-L-2-deoxyfucose with aklavinone as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C Streptomyces galilaeus
2.4.1.326 0.4072
-
 dTDP-4-amino-2-deoxyrhamnose in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C Streptomyces galilaeus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.326 dTDP-beta-L-rhodosamine + aklavinone Streptomyces galilaeus
-
 dTDP + aclacinomycin T
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.326 Streptomyces galilaeus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.326 Ni-NTA affinity column chromatography, and gel filtration Streptomyces galilaeus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.326 dTDP-4-amino-2-deoxyrhamnose + aklavinone
-
 Streptomyces galilaeus dTDP + 4-O-(2,4-dideoxy-4-amino-alpha-L-rhamnopyranosyl)aklavinone
-
 ?
2.4.1.326 dTDP-4-amino-2-deoxyrhamnose + aklavinone
-
 Streptomyces galilaeus dTDP + 4-O-(2,4-dideoxy-4-amino-alpha-L-rhamnosyl)aklavinone
-
 ?
2.4.1.326 dTDP-beta-L-rhodosamine + aklavinone
-
 Streptomyces galilaeus dTDP + aclacinomycin T
-
 ?
2.4.1.326 dTDP-L-2-deoxyfucose + aklavinone
-
 Streptomyces galilaeus dTDP + 4-O-(2-deoxy-alpha-L-fucosyl)aklavinone
-
 ?
2.4.1.326 dTDP-L-2-deoxyfucose + epsilon-rhodomycinone
-
 Streptomyces galilaeus dTDP + 4-O-(2-deoxy-alpha-L-fucosyl)-epsilon-rhodomycinone
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.326 AknS
-
 Streptomyces galilaeus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.326 0.001
-
 epsilon-rhodomycinone with dTDP-L-2-deoxyfucose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C Streptomyces galilaeus
2.4.1.326 0.001
-
 dTDP-L-2-deoxyfucose with epsilon-rhodomycinone as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C Streptomyces galilaeus
2.4.1.326 0.003
-
 dTDP-4-amino-2-deoxyrhamnose in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C Streptomyces galilaeus
2.4.1.326 0.003
-
 aklavinone with dTDP-4-amino-2-deoxyrhamnose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C Streptomyces galilaeus
2.4.1.326 0.004
-
 dTDP-L-2-deoxyfucose with aklavinone as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C Streptomyces galilaeus
2.4.1.326 0.004
-
 aklavinone with dTDP-L-2-deoxyfucose as cosubstrate, in 75 mM Tris (pH 7.5), 10 mM MgCl2, at 25°C Streptomyces galilaeus