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Literature summary extracted from

  • Yang, D.D.; Francois, J.M.; de Billerbeck, G.M.
    Cloning, expression and characterization of an aryl-alcohol dehydrogenase from the white-rot fungus Phanerochaete chrysosporium strain BKM-F-1767 (2012), BMC Microbiol., 12, 126.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.91 expressed in Escherichia coli as a His-tagged fusion protein Phanerodontia chrysosporium

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.91 3,4-dimethoxybenzaldehyde
-
 Phanerodontia chrysosporium
1.1.1.91 3,4-dimethoxybenzyl alcohol
-
 Phanerodontia chrysosporium
1.1.1.91 3,5-dimethoxybenzaldehyde
-
 Phanerodontia chrysosporium
1.1.1.91 3-hydroxy-4-methoxy-benzaldehyde
-
 Phanerodontia chrysosporium
1.1.1.91 4-hydroxy-3-methoxybenzaldehyde
-
 Phanerodontia chrysosporium
1.1.1.91 5-(hydroxymethyl)-2-furaldehyde
-
 Phanerodontia chrysosporium
1.1.1.91 benzaldehyde
-
 Phanerodontia chrysosporium
1.1.1.91 trans-cinnamaldehyde
-
 Phanerodontia chrysosporium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.91 0.012
-
 3,4-dimethoxybenzaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 0.022
-
 3,5-dimethoxybenzaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 0.038
-
 NADP+ pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 0.039
-
 NADPH pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 0.09
-
 4-methoxybenzaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 0.22
-
 NADH pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 0.27
-
 5-(hydroxymethyl)-2-furaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 0.37
-
 3,4-dimethoxybenzyl alcohol pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 0.53
-
 phenylacetaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 1.4
-
 4-Hydroxy-3-methoxy-benzaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 1.4
-
 3-hydroxy-4-methoxy-benzaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 1.7
-
 benzaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 3.4
-
 trans-cinnamaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 25
-
 4-hydroxy-3-methoxy-benzyl alcohol pH 6.1, 30°C Phanerodontia chrysosporium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.91 40000
-
 SDS-PAGE Phanerodontia chrysosporium

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.91 Phanerodontia chrysosporium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.91 using Ni-NTA chromatography Phanerodontia chrysosporium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.91 2-fluorobenzaldehyde + NADPH relative activity: 27% (reference substrate: 3,4-dimethoxybenzaldehyde) Phanerodontia chrysosporium 2-fluorobenzyl alcohol + NADP+
-
 r
1.1.1.91 2-methoxybenzaldehyde + NADPH relative activity: 58% (reference substrate: 3,4-dimethoxybenzaldehyde) Phanerodontia chrysosporium 2-methoxybenzyl alcohol + NADP+
-
 r
1.1.1.91 2-nitrobenzaldehyde + NADPH relative activity: 57% (reference substrate: 3,4-dimethoxybenzaldehyde) Phanerodontia chrysosporium 2-nitrobenzyl alcohol + NADP+
-
 r
1.1.1.91 3,4-dimethoxybenzaldehyde + NADH
-
 Phanerodontia chrysosporium 3,4-dimethoxybenzyl alcohol + NAD+
-
 r
1.1.1.91 3,4-dimethoxybenzaldehyde + NADPH relative activity: 100% (reference substrate: 3,4-dimethoxybenzaldehyde) Phanerodontia chrysosporium 3,4-dimethoxybenzyl alcohol + NADP+
-
 r
1.1.1.91 3,5-dimethoxybenzaldehyde + NADPH relative activity: 110% (reference substrate: 3,4-dimethoxybenzaldehyde) Phanerodontia chrysosporium 3,5-dimethoxybenzyl alcohol + NADP+
-
 r
1.1.1.91 3-chlorobenzaldehyde + NADPH relative activity: 73% (reference substrate: 3,4-dimethoxybenzaldehyde) Phanerodontia chrysosporium 3-chlorobenzyl alcohol + NADP+
-
 r
1.1.1.91 3-fluorobenzaldehyde + NADPH relative activity: 69% (reference substrate: 3,4-dimethoxybenzaldehyde) Phanerodontia chrysosporium 3-fluorobenzyl alcohol + NADP+
-
 r
1.1.1.91 3-hydroxy-4-methoxy-benzaldehyde + NADPH relative activity: 17% (reference substrate: 3,4-dimethoxybenzaldehyde) Phanerodontia chrysosporium 3-hydroxy-4-methoxy-benzyl alcohol + NADP+
-
 r
1.1.1.91 3-methoxybenzaldehyde + NADPH relative activity: 117% (reference substrate: 3,4-dimethoxybenzaldehyde) Phanerodontia chrysosporium 3-methoxybenzyl alcohol + NADP+
-
 r
1.1.1.91 3-nitrobenzaldehyde + NADPH relative activity: 25% (reference substrate: 3,4-dimethoxybenzaldehyde) Phanerodontia chrysosporium 3-nitrobenzyl alcohol + NADP+
-
 r
1.1.1.91 4-chlorobenzaldehyde + NADPH relative activity: 61% (reference substrate: 3,4-dimethoxybenzaldehyde) Phanerodontia chrysosporium 4-chlorobenzyl alcohol + NADP+
-
 r
1.1.1.91 4-fluorobenzaldehyde + NADPH relative activity: 29% (reference substrate: 3,4-dimethoxybenzaldehyde) Phanerodontia chrysosporium 4-fluorobenzyl alcohol + NADP+
-
 r
1.1.1.91 4-hydroxy-3-methoxy-benzaldehyde + NADPH relative activity: 19% (reference substrate: 3,4-dimethoxybenzaldehyde) Phanerodontia chrysosporium 4-hydroxy-3-methoxy-benzyl alcohol + NADP+
-
 r
1.1.1.91 4-hydroxybenzaldehyde + NADPH relative activity: 34% (reference substrate: 3,4-dimethoxybenzaldehyde) Phanerodontia chrysosporium 4-hydroxybenzyl alcohol + NADP+
-
 r
1.1.1.91 4-methoxybenzaldehyde + NADPH relative activity: 110% (reference substrate: 3,4-dimethoxybenzaldehyde) Phanerodontia chrysosporium 4-methoxybenzyl alcohol + NADP+
-
 r
1.1.1.91 4-nitrobenzaldehyde + NADPH relative activity: 31% (reference substrate: 3,4-dimethoxybenzaldehyde) Phanerodontia chrysosporium 4-nitrobenzyl alcohol + NADP+
-
 r
1.1.1.91 5-(hydroxymethyl)-2-furaldehyde + NADPH + H+ relative activity: 36% (reference substrate: 3,4-dimethoxybenzaldehyde) Phanerodontia chrysosporium (furan-2,5-diyl)dimethanol + NADP+
-
 r
1.1.1.91 benzaldehyde + NADPH relative activity: 42% (reference substrate: 3,4-dimethoxybenzaldehyde) Phanerodontia chrysosporium benzyl alcohol + NADP+
-
 r
1.1.1.91 decanal + NADPH relative activity: 2% (reference substrate: 3,4-dimethoxybenzaldehyde) Phanerodontia chrysosporium decanol + NADP+
-
 r
1.1.1.91 heptaldehyde + NADPH relative activity: 26% (reference substrate: 3,4-dimethoxybenzaldehyde) Phanerodontia chrysosporium ?
-
 r
1.1.1.91 hexanal + NADPH relative activity: 47% (reference substrate: 3,4-dimethoxybenzaldehyde) Phanerodontia chrysosporium hexanol + NADP+
-
 r
1.1.1.91 nonanal + NADPH relative activity: 3% (reference substrate: 3,4-dimethoxybenzaldehyde) Phanerodontia chrysosporium nonanol + NADP+
-
 r
1.1.1.91 octanal + NADPH relative activity: 37% (reference substrate: 3,4-dimethoxybenzaldehyde) Phanerodontia chrysosporium octanol + NADP+
-
 r
1.1.1.91 pentanal + NADPH relative activity: 8% (reference substrate: 3,4-dimethoxybenzaldehyde) Phanerodontia chrysosporium pentanol + NADP+
-
 r
1.1.1.91 phenylacetaldehyde + NADPH relative activity: 109 (reference substrate: 3,4-dimethoxybenzaldehyde) Phanerodontia chrysosporium phenylethanol + NADP+
-
 r
1.1.1.91 trans-cinnamaldehyde + NADPH relative activity: 39% (reference substrate: 3,4-dimethoxybenzaldehyde) Phanerodontia chrysosporium ?
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.91 Aad1p
-
 Phanerodontia chrysosporium
1.1.1.91 aryl-alcohol dehydrogenase
-
 Phanerodontia chrysosporium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.91 37
-
 assay at Phanerodontia chrysosporium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.91 2
-
 NADH pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 2.55
-
 3,4-dimethoxybenzyl alcohol pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 2.56
-
 NADP+ pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 2.93
-
 5-(hydroxymethyl)-2-furaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 3.33
-
 4-Hydroxy-3-methoxy-benzaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 3.83
-
 3-hydroxy-4-methoxy-benzaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 4.33
-
 4-hydroxy-3-methoxy-benzyl alcohol pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 7.16
-
 benzaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 8.16
-
 4-methoxybenzaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 8.833
-
 3,4-dimethoxybenzaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 9.83
-
 3,5-dimethoxybenzaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 11.16
-
 phenylacetaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 11.16
-
 trans-cinnamaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 11.33
-
 NADPH pH 6.1, 30°C Phanerodontia chrysosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.91 6.4
-
 reduction activity Phanerodontia chrysosporium
1.1.1.91 10.4
-
 oxidation activity Phanerodontia chrysosporium

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.91 2.1
-
 3,5-dimethoxybenzaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 2.3
-
 3-hydroxy-4-methoxy-benzaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 3.4
-
 3,4-dimethoxybenzaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 3.5
-
 trans-cinnamaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 5.1
-
 4-hydroxy-3-methoxybenzaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 81
-
 benzaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 136
-
 5-(hydroxymethyl)-2-furaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 165
-
 3,4-dimethoxybenzyl alcohol pH 6.1, 30°C Phanerodontia chrysosporium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.91 0.166
-
 4-hydroxy-3-methoxy-benzyl alcohol pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 2.33
-
 4-Hydroxy-3-methoxy-benzaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 2.66
-
 3-hydroxy-4-methoxy-benzaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 3.33
-
 trans-cinnamaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 5
-
 benzaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 6.8
-
 3,4-dimethoxybenzyl alcohol pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 10
-
 NADH pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 10
-
 5-(hydroxymethyl)-2-furaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 11.16
-
 phenylacetaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 68
-
 NADP+ pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 90
-
 4-methoxybenzaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 283
-
 NADPH pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 450
-
 3,5-dimethoxybenzaldehyde pH 6.1, 30°C Phanerodontia chrysosporium
1.1.1.91 733
-
 3,4-dimethoxybenzaldehyde pH 6.1, 30°C Phanerodontia chrysosporium