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Steady-state generation of hydrogen peroxide: kinetics and stability of alcohol oxidase immobilized on nanoporous alumina

Kjellander, M.; Goetz, K.; Liljeruhm, J.; Boman, M.; Johansson, G.; Biotechnol. Lett. 35, 585-590 (2013)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.1.3.13
additional information
the alcohol oxidase is immobilized on nanoporous aluminium oxide membranes by silanization and activation by carbonyldiimidazole to create a flow-through enzyme reactor, method optimization, overview. The immobilized alcohol oxidase shows high productivity in hydrogen peroxide generation from methanol, and retains about 58% of initial activity after 3 weeks of storage and repeated use with pH 6.5 and at room temperature. Construction of a bienzymatic modular reactor with horseradish peroxidase and alcohol oxidase, overview
Komagataella pastoris
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.3.13
additional information
-
additional information
Michaelis-Menten kinetics, overview
Komagataella pastoris
1.1.3.13
0.47
-
methanol
pH 6.5, 22C, immobilized enzyme
Komagataella pastoris
1.1.3.13
0.75
-
ethanol
pH 6.5, 22C, immobilized enzyme
Komagataella pastoris
1.1.3.13
1.23
-
n-Propanol
pH 6.5, 22C, immobilized enzyme
Komagataella pastoris
1.1.3.13
1.59
-
formaldehyde
pH 6.5, 22C, immobilized enzyme
Komagataella pastoris
1.1.3.13
2.79
-
n-butanol
pH 6.5, 22C, immobilized enzyme
Komagataella pastoris
1.1.3.13
8
9
ethylene glycol
pH 6.5, 22C, immobilized enzyme
Komagataella pastoris
1.1.3.13
261
-
2-propanol
pH 6.5, 22C, immobilized enzyme
Komagataella pastoris
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.3.13
80000
-
4 * 80000, SDS-PAGE
Komagataella pastoris
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.3.13
methanol + O2
Komagataella pastoris
-
formaldehyde + H2O2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.3.13
Komagataella pastoris
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.3.13
2-propanol + O2
-
724647
Komagataella pastoris
2-oxopropane + H2O2
-
-
-
?
1.1.3.13
ethanol + O2
-
724647
Komagataella pastoris
acetaldehyde + H2O2
-
-
-
?
1.1.3.13
ethylene glycol + O2
-
724647
Komagataella pastoris
? + H2O2
-
-
-
?
1.1.3.13
formaldehyde + O2
-
724647
Komagataella pastoris
formic acid + H2O2
-
-
-
?
1.1.3.13
methanol + O2
-
724647
Komagataella pastoris
formaldehyde + H2O2
-
-
-
?
1.1.3.13
additional information
substrate specificity, overview. No activity with isobutanol, benzyl alcohol, and cyclohexanol
724647
Komagataella pastoris
?
-
-
-
-
1.1.3.13
n-butanol + O2
-
724647
Komagataella pastoris
butanal + H2O2
-
-
-
?
1.1.3.13
n-propanol + O2
-
724647
Komagataella pastoris
propanal + H2O2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.3.13
homotetramer
4 * 80000, SDS-PAGE
Komagataella pastoris
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.3.13
22
-
assay at room temperature
Komagataella pastoris
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.3.13
6.5
-
assay at
Komagataella pastoris
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.3.13
FAD
-
Komagataella pastoris
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.3.13
FAD
-
Komagataella pastoris
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.1.3.13
additional information
the alcohol oxidase is immobilized on nanoporous aluminium oxide membranes by silanization and activation by carbonyldiimidazole to create a flow-through enzyme reactor, method optimization, overview. The immobilized alcohol oxidase shows high productivity in hydrogen peroxide generation from methanol, and retains about 58% of initial activity after 3 weeks of storage and repeated use with pH 6.5 and at room temperature. Construction of a bienzymatic modular reactor with horseradish peroxidase and alcohol oxidase, overview
Komagataella pastoris
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.3.13
additional information
-
additional information
Michaelis-Menten kinetics, overview
Komagataella pastoris
1.1.3.13
0.47
-
methanol
pH 6.5, 22C, immobilized enzyme
Komagataella pastoris
1.1.3.13
0.75
-
ethanol
pH 6.5, 22C, immobilized enzyme
Komagataella pastoris
1.1.3.13
1.23
-
n-Propanol
pH 6.5, 22C, immobilized enzyme
Komagataella pastoris
1.1.3.13
1.59
-
formaldehyde
pH 6.5, 22C, immobilized enzyme
Komagataella pastoris
1.1.3.13
2.79
-
n-butanol
pH 6.5, 22C, immobilized enzyme
Komagataella pastoris
1.1.3.13
8
9
ethylene glycol
pH 6.5, 22C, immobilized enzyme
Komagataella pastoris
1.1.3.13
261
-
2-propanol
pH 6.5, 22C, immobilized enzyme
Komagataella pastoris
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.3.13
80000
-
4 * 80000, SDS-PAGE
Komagataella pastoris
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.3.13
methanol + O2
Komagataella pastoris
-
formaldehyde + H2O2
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.3.13
2-propanol + O2
-
724647
Komagataella pastoris
2-oxopropane + H2O2
-
-
-
?
1.1.3.13
ethanol + O2
-
724647
Komagataella pastoris
acetaldehyde + H2O2
-
-
-
?
1.1.3.13
ethylene glycol + O2
-
724647
Komagataella pastoris
? + H2O2
-
-
-
?
1.1.3.13
formaldehyde + O2
-
724647
Komagataella pastoris
formic acid + H2O2
-
-
-
?
1.1.3.13
methanol + O2
-
724647
Komagataella pastoris
formaldehyde + H2O2
-
-
-
?
1.1.3.13
additional information
substrate specificity, overview. No activity with isobutanol, benzyl alcohol, and cyclohexanol
724647
Komagataella pastoris
?
-
-
-
-
1.1.3.13
n-butanol + O2
-
724647
Komagataella pastoris
butanal + H2O2
-
-
-
?
1.1.3.13
n-propanol + O2
-
724647
Komagataella pastoris
propanal + H2O2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.3.13
homotetramer
4 * 80000, SDS-PAGE
Komagataella pastoris
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.3.13
22
-
assay at room temperature
Komagataella pastoris
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.3.13
6.5
-
assay at
Komagataella pastoris