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Literature summary extracted from

  • Sharafi, A.; Sohi, H.H.; Mousavi, A.; Azadi, P.; Khalifani, B.H.; Razavi, K.
    Metabolic engineering of morphinan alkaloids by over-expression of codeinone reductase in transgenic hairy roots of Papaver bracteatum, the Iranian poppy (2013), Biotechnol. Lett., 35, 445-453.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.247 synthesis Papaver bracteatum hairy roots expressing CodR gene have a high potential to produce morphinan alkaloids Papaver somniferum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.247 gene codR, expression in Papaver bracteatum in cultured hairy roots, subcloning in Escherichia coli strain DH5alpha. The T-DNA of the vector contains a neomycin phosphoteransferase gene and CodR cDNA with CaMV35S promoter and nos terminator Papaver somniferum

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.247 additional information transgenic codeinone reductase overexpressing Papaver somniferum plants show 75% increase in thebaine besides 58% and 22% increases in codeine and morphine, respectively, compared to the wild-type plants in the field trail Papaver somniferum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.247 codeinone + NADPH + H+ Papaver somniferum
-
 codeine + NADP+
-
 r
1.1.1.247 additional information Papaver somniferum the enzyme also catalyzes the NADPH-dependent reduction of morphinone to morphine, reaction of EC 1.1.1.218. but codeine can also be directly converted into morphine, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.247 Papaver somniferum Q9SQ67
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.247 leaf
-
 Papaver somniferum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.247 codeinone + NADPH + H+
-
 Papaver somniferum codeine + NADP+
-
 r
1.1.1.247 additional information the enzyme also catalyzes the NADPH-dependent reduction of morphinone to morphine, reaction of EC 1.1.1.218. but codeine can also be directly converted into morphine, overview Papaver somniferum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.247 codeinone reductase (NADPH)
-
 Papaver somniferum
1.1.1.247 CodR
-
 Papaver somniferum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.247 NADP+
-
 Papaver somniferum
1.1.1.247 NADPH
-
 Papaver somniferum