Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Liu, Y.; Pan, J.; Wei, P.; Zhu, J.; Huang, L.; Cai, J.; Xu, Z.
    Efficient expression and purification of recombinant alcohol oxidase in Pichia pastoris (2012), Biotechnol. Bioprocess Eng., 17, 693-702.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.13 functional recombinant expression of C-terminally His6-tagged enzyme in Pichia pastoris strain GS115 from the plasmid pPIC9K-AOX intracellularly and extracellularly, batch culture. Expression in Escherichia coli strain DH5alpha in batch culture. Method comparison and optimization, overview Komagataella pastoris

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.13 H2O2 inhibits at 5 mM, at 7.8 mM the enzyme maintains 50% of its initial activity after 0.5 h of incubation at 4°C Komagataella pastoris

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.1.3.13 Ethanol pH 8.0, activates at 30% v/v for 0.5 h, then inhibits after 8 h Komagataella pastoris
1.1.3.13 hexanol pH 8.0, activates at 30% v/v for 0.5 h, then inhibits slightly after 8 h Komagataella pastoris
1.1.3.13 isoamyl alcohol pH 8.0, activates at 30% v/v for 0.5 h, then inhibits after 8 h Komagataella pastoris
1.1.3.13 isobutanol pH 8.0, only slight effect on enzyme activity at 30% v/v Komagataella pastoris
1.1.3.13 isopropanol pH 8.0, activates at 30% v/v for 3 h, then inhibits strongly up after h Komagataella pastoris
1.1.3.13 Methanol pH 8.0, activates at 30% v/v for 0.5 h, then inhibits after 8 h Komagataella pastoris
1.1.3.13 additional information effects of various solvents on the stability and activity of recmbinant AOX, overview Komagataella pastoris
1.1.3.13 n-Butanol pH 8.0, activates at 30% v/v for 0.5 h, then inhibits after 8 h Komagataella pastoris

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.13 Komagataella pastoris
-
-
-
1.1.3.13 Komagataella pastoris GS115
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.13 recombinant C-terminally His6-tagged enzyme from Pichia pastoris strain GS115 by nickel affinity chromatography and gel filtration. The recombinant enzyme shows high thermal stability and resistance to H2O2 inhibition Komagataella pastoris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.13 11.85
-
purified recombinant intracellular enzyme, pH 80, 25°C Komagataella pastoris
1.1.3.13 12.94
-
purified recombinant extracellular enzyme, pH 80, 25°C Komagataella pastoris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.13 1,2-propanediol + O2
-
Komagataella pastoris ? + H2O2
-
?
1.1.3.13 1,3-butylene glycol + O2
-
Komagataella pastoris ? + H2O2
-
?
1.1.3.13 1,3-butylene glycol + O2
-
Komagataella pastoris GS115 ? + H2O2
-
?
1.1.3.13 2-propanol + O2
-
Komagataella pastoris 2-oxopropane + H2O2
-
?
1.1.3.13 ethanol + O2
-
Komagataella pastoris acetaldehyde + H2O2
-
?
1.1.3.13 ethanol + O2
-
Komagataella pastoris GS115 acetaldehyde + H2O2
-
?
1.1.3.13 hexanol + O2
-
Komagataella pastoris hexanal + H2O2
-
?
1.1.3.13 hexanol + O2
-
Komagataella pastoris GS115 hexanal + H2O2
-
?
1.1.3.13 methanol + O2
-
Komagataella pastoris formaldehyde + H2O2
-
?
1.1.3.13 methanol + O2
-
Komagataella pastoris GS115 formaldehyde + H2O2
-
?
1.1.3.13 n-butanol + O2
-
Komagataella pastoris butanal + H2O2
-
?
1.1.3.13 n-butanol + O2
-
Komagataella pastoris GS115 butanal + H2O2
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.13 AOX
-
Komagataella pastoris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.13 37
-
-
Komagataella pastoris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.13 42
-
purified recombinant His-tagged enzyme, pH 8.0, 60 min, stable Komagataella pastoris
1.1.3.13 50
-
purified recombinant His-tagged enzyme, pH 8.0, 50 min, 50% activity remaining Komagataella pastoris
1.1.3.13 55
-
purified recombinant His-tagged enzyme, pH 8.0, 30 min, inactivation Komagataella pastoris

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.13 4.2
-
purified recombinant His-tagged enzyme, 25°C, 3 h, inactivation Komagataella pastoris
1.1.3.13 5.5 7 purified recombinant His-tagged enzyme, 25°C, 3 h, 50% activity remaining Komagataella pastoris
1.1.3.13 8 10 purified recombinant His-tagged enzyme, 25°C, 3 h, stable Komagataella pastoris