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Literature summary extracted from

  • Sun, J.; Morita, H.; Chen, G.; Noguchi, H.; Abe, I.
    Molecular cloning and characterization of copper amine oxidase from Huperzia serrata (2012), Bioorg. Med. Chem. Lett., 22, 5784-5790.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.21 expressed in Escherichia coli BL21(DE3)pLysS cells Huperzia serrata

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.21 F190Y the mutant loses the activity within the range of pH 6.8-8.5 Huperzia serrata
1.4.3.21 F384G the mutant loses the activity within the range of pH 6.8-8.5 Huperzia serrata
1.4.3.21 I160F the mutant loses the activity within the range of pH 6.8-8.5 Huperzia serrata
1.4.3.21 S406T the mutant loses the activity within the range of pH 6.8-8.5 Huperzia serrata
1.4.3.21 Y320F the mutant loses the activity within the range of pH 6.8-8.5 Huperzia serrata
1.4.3.21 Y326F the mutant loses the activity within the range of pH 6.8-8.5 Huperzia serrata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.21 0.3
-
 cadaverine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.4.3.21 0.5
-
 benzylamine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.4.3.21 0.7
-
 histamine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.4.3.21 0.8
-
 spermidine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.4.3.21 1.2
-
 putrescine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.4.3.21 1.2
-
 3-aminomethylpyridine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.4.3.21 1.7
-
 2-aminoethylpyridine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.4.3.21 1.9
-
 4-aminomethylpyridine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.3.21 Cu2+ required for activity Huperzia serrata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.21 77676
-
 2 * 77676, calculated from amino acid sequence Huperzia serrata
1.4.3.21 78000
-
 2 * 78000, SDS-PAGE Huperzia serrata
1.4.3.21 162000
-
 gel filtration Huperzia serrata

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.21 Huperzia serrata I6NC69 Chinese club moss
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.21 Ni-chelating affinity column chromatography Huperzia serrata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.21 2-aminoethylpyridine + H2O + O2
-
 Huperzia serrata pyridine-2-carbaldehyde + NH3 + H2O2
-
 ?
1.4.3.21 3-aminomethylpyridine + H2O + O2
-
 Huperzia serrata pyridine-3-carbaldehyde + NH3 + H2O2
-
 ?
1.4.3.21 4-aminomethylpyridine + H2O + O2
-
 Huperzia serrata pyridine-4-carbaldehyde + NH3 + H2O2
-
 ?
1.4.3.21 benzylamine + H2O + O2
-
 Huperzia serrata benzaldehyde + NH3 + H2O2
-
 ?
1.4.3.21 cadaverine + H2O + O2 best substrate Huperzia serrata DELTA1-piperideine + NH3 + H2O2
-
 ?
1.4.3.21 histamine + H2O + O2
-
 Huperzia serrata (imidazol-4-yl)acetaldehyde + NH3 + H2O2
-
 ?
1.4.3.21 putrescine + H2O + O2
-
 Huperzia serrata ?
-
 ?
1.4.3.21 spermidine + H2O + O2
-
 Huperzia serrata ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.21 homodimer 2 * 78000, SDS-PAGE Huperzia serrata
1.4.3.21 homodimer 2 * 77676, calculated from amino acid sequence Huperzia serrata

Synonyms

EC Number Synonyms Comment Organism
1.4.3.21 CAO
-
 Huperzia serrata
1.4.3.21 Copper amine oxidase
-
 Huperzia serrata

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.21 1
-
 cadaverine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.4.3.21 1.4
-
 benzylamine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.4.3.21 1.8
-
 histamine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.4.3.21 1.9
-
 putrescine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.4.3.21 1.9
-
 3-aminomethylpyridine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.4.3.21 2.3
-
 spermidine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.4.3.21 2.9
-
 2-aminoethylpyridine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.4.3.21 3.1
-
 4-aminomethylpyridine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.21 8
-
 pH optimum for the piperideine-forming activity oft he enzyme Huperzia serrata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.3.21 6.8 8.5 piperideine-forming activity Huperzia serrata

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.21 2,4,5-trihydroxyphenylalanine quinone
-
 Huperzia serrata

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.3.21 1.6
-
 putrescine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.4.3.21 1.6
-
 4-aminomethylpyridine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.4.3.21 1.6
-
 3-aminomethylpyridine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.4.3.21 1.7
-
 2-aminoethylpyridine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.4.3.21 2.6
-
 histamine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.4.3.21 2.8
-
 benzylamine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.4.3.21 2.9
-
 spermidine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata
1.4.3.21 3.3
-
 cadaverine in 50 mM Tris-HCl buffer, pH 8.0, at 25°C Huperzia serrata