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Literature summary extracted from

  • Fujii, T.; Shimizu, M.; Doi, Y.; Fujita, T.; Ito, T.; Miura, D.; Wariishi, H.; Takaya, N.
    Novel fungal phenylpyruvate reductase belongs to D-isomer-specific 2-hydroxyacid dehydrogenase family (2011), Biochim. Biophys. Acta, 1814, 1669-1676.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.237 expression in Escherichia coli Wickerhamia fluorescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.237 Cu2+ 1 mM, less than 10% residual activity Wickerhamia fluorescens
1.1.1.237 Fe2+ 1 mM, less than 10% residual activity Wickerhamia fluorescens
1.1.1.237 Hg2+ 1 mM, less than 10% residual activity Wickerhamia fluorescens
1.1.1.237 WO42- 1 mM, less than 10% residual activity Wickerhamia fluorescens
1.1.1.237 Zn2+ 1 mM, less than 10% residual activity Wickerhamia fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.237 0.01
-
 NADPH pH 6.5, 25°C Wickerhamia fluorescens
1.1.1.237 0.1
-
 NADH pH 6.5, 25°C Wickerhamia fluorescens
1.1.1.237 0.4
-
 phenylpyruvate pH 6.5, 25°C Wickerhamia fluorescens
1.1.1.237 0.64
-
 hydroxyphenylpyruvate pH 6.5, 25°C Wickerhamia fluorescens
1.1.1.237 3.5
-
 Hydroxypyruvate pH 6.5, 25°C Wickerhamia fluorescens
1.1.1.237 18.9
-
 glyoxylate pH 6.5, 25°C Wickerhamia fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.237 40000
-
 2 * 40000, SDS-PAGE, 2 * 40300, calculated Wickerhamia fluorescens
1.1.1.237 40300
-
 2 * 40000, SDS-PAGE, 2 * 40300, calculated Wickerhamia fluorescens
1.1.1.237 75000
-
 gel filtration Wickerhamia fluorescens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.237 Wickerhamia fluorescens F1T2J9
-
-
1.1.1.237 Wickerhamia fluorescens TK1 F1T2J9
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.237 727
-
 pH 6.5, 25°C Wickerhamia fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.237 4-hydroxyphenylpyruvate + NADPH + H+
-
 Wickerhamia fluorescens D-(4-hydroxyphenyl)lactate + NADP+
-
 ?
1.1.1.237 4-hydroxyphenylpyruvate + NADPH + H+
-
 Wickerhamia fluorescens TK1 D-(4-hydroxyphenyl)lactate + NADP+
-
 ?
1.1.1.237 glyoxylate + NADPH + H+
-
 Wickerhamia fluorescens glycolate + NADP+
-
 ?
1.1.1.237 glyoxylate + NADPH + H+
-
 Wickerhamia fluorescens TK1 glycolate + NADP+
-
 ?
1.1.1.237 hydroxypyruvate + NADH + H+
-
 Wickerhamia fluorescens D-glycerate + NAD+
-
 ?
1.1.1.237 hydroxypyruvate + NADH + H+
-
 Wickerhamia fluorescens TK1 D-glycerate + NAD+
-
 ?
1.1.1.237 additional information no substrates: pyruvate, oxaloacetate or benzoylformate Wickerhamia fluorescens ?
-
 ?
1.1.1.237 additional information no substrates: pyruvate, oxaloacetate or benzoylformate Wickerhamia fluorescens TK1 ?
-
 ?
1.1.1.237 phenylpyruvate + NADPH + H+ more than 99.9% D-isomer, L-isomer below limits of detection Wickerhamia fluorescens D-phenyllactate + NADP+
-
 ?
1.1.1.237 phenylpyruvate + NADPH + H+ more than 99.9% D-isomer, L-isomer below limits of detection Wickerhamia fluorescens TK1 D-phenyllactate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.237 dimer 2 * 40000, SDS-PAGE, 2 * 40300, calculated Wickerhamia fluorescens

Synonyms

EC Number Synonyms Comment Organism
1.1.1.237 Ppr
-
 Wickerhamia fluorescens
1.1.1.237 pprA
-
 Wickerhamia fluorescens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.237 9.1
-
 Hydroxypyruvate pH 6.5, 25°C Wickerhamia fluorescens
1.1.1.237 19
-
 glyoxylate pH 6.5, 25°C Wickerhamia fluorescens
1.1.1.237 31
-
 NADH pH 6.5, 25°C Wickerhamia fluorescens
1.1.1.237 73
-
 hydroxyphenylpyruvate pH 6.5, 25°C Wickerhamia fluorescens
1.1.1.237 121
-
 NADPH pH 6.5, 25°C Wickerhamia fluorescens
1.1.1.237 150
-
 phenylpyruvate pH 6.5, 25°C Wickerhamia fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.237 6.5 7
-
 Wickerhamia fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.237 NAD+
-
 Wickerhamia fluorescens
1.1.1.237 NADH NADPH is preferred over NADH Wickerhamia fluorescens
1.1.1.237 NADP+
-
 Wickerhamia fluorescens
1.1.1.237 NADPH preferred over NADH Wickerhamia fluorescens

Expression

EC Number Organism Comment Expression
1.1.1.237 Wickerhamia fluorescens presence of phenylalanine results in up to 40fold increase in transcripts up

General Information

EC Number General Information Comment Organism
1.1.1.237 physiological function Wickerhamia fluorescens efficiently converts phenylalanine and phenylpyruvate to D-phenyllactate. These compounds up-regulate the transcription of enzyme gene pprA Wickerhamia fluorescens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.237 1
-
 glyoxylate pH 6.5, 25°C Wickerhamia fluorescens
1.1.1.237 2.6
-
 Hydroxypyruvate pH 6.5, 25°C Wickerhamia fluorescens
1.1.1.237 110
-
 hydroxyphenylpyruvate pH 6.5, 25°C Wickerhamia fluorescens
1.1.1.237 310
-
 NADH pH 6.5, 25°C Wickerhamia fluorescens
1.1.1.237 380
-
 phenylpyruvate pH 6.5, 25°C Wickerhamia fluorescens
1.1.1.237 12000
-
 NADPH pH 6.5, 25°C Wickerhamia fluorescens