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Literature summary extracted from

  • Horiuchi, Y.; Ohmae, E.; Tate, S.; Gekko, K.
    Coupling effects of distal loops on structural stability and enzymatic activity of Escherichia coli dihydrofolate reductase revealed by deletion mutants (2010), Biochim. Biophys. Acta, 1804, 846-855.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.3 expressed in Escherichia coli HB101 cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.5.1.3 DELTAAla145 the mutant shows increased catalytic efficiency compared to the wild type enzyme Escherichia coli
1.5.1.3 DELTAArg52 the mutant shows reduced catalytic efficiency compared to the wild type enzyme Escherichia coli
1.5.1.3 DELTAArg52/DELTAAla145 the mutant shows reduced catalytic efficiency compared to the wild type enzyme Escherichia coli
1.5.1.3 DELTAArg52/DELTAGly121 the mutant shows reduced catalytic efficiency compared to the wild type enzyme Escherichia coli
1.5.1.3 DELTAArg52/DELTAGly67 the mutant shows reduced catalytic efficiency compared to the wild type enzyme Escherichia coli
1.5.1.3 DELTAGly121 the mutant shows reduced catalytic efficiency compared to the wild type enzyme Escherichia coli
1.5.1.3 DELTAGly121/DELTAAla145 the mutant shows reduced catalytic efficiency compared to the wild type enzyme Escherichia coli
1.5.1.3 DELTAGly67 the mutant shows reduced catalytic efficiency compared to the wild type enzyme Escherichia coli
1.5.1.3 DELTAGly67/DELTAAla145 the mutant shows reduced catalytic efficiency compared to the wild type enzyme Escherichia coli
1.5.1.3 DELTAGly67/DELTAGly121 the mutant shows reduced catalytic efficiency compared to the wild type enzyme Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 0.0008
-
 7,8-dihydrofolate deletion mutant DELTAAla145, at pH 7.0 and 25°C Escherichia coli
1.5.1.3 0.0013
-
 7,8-dihydrofolate wild type enzyme, at pH 7.0 and 25°C Escherichia coli
1.5.1.3 0.0048
-
 7,8-dihydrofolate deletion mutant DELTAGly67/DELTAAla145, at pH 7.0 and 25°C Escherichia coli
1.5.1.3 0.0051
-
 7,8-dihydrofolate deletion mutant DELTAGly67, at pH 7.0 and 25°C Escherichia coli
1.5.1.3 0.0056
-
 7,8-dihydrofolate deletion mutant DELTAGly67/DELTAGly121, at pH 7.0 and 25°C Escherichia coli
1.5.1.3 0.0073
-
 7,8-dihydrofolate deletion mutant DELTAGly121/DELTAAla145, at pH 7.0 and 25°C Escherichia coli
1.5.1.3 0.0108
-
 7,8-dihydrofolate deletion mutant DELTAGly121, at pH 7.0 and 25°C Escherichia coli
1.5.1.3 0.0502
-
 7,8-dihydrofolate deletion mutant DELTAArg52/DELTAGly67, at pH 7.0 and 25°C Escherichia coli
1.5.1.3 0.0512
-
 7,8-dihydrofolate deletion mutant DELTAArg52/DELTAGly121, at pH 7.0 and 25°C Escherichia coli
1.5.1.3 0.0777
-
 7,8-dihydrofolate deletion mutant DELTAArg52/DELTAAla145, at pH 7.0 and 25°C Escherichia coli
1.5.1.3 0.1161
-
 7,8-dihydrofolate deletion mutant DELTAArg52, at pH 7.0 and 25°C Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH + H+ Escherichia coli
-
 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Escherichia coli P0ABQ4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH + H+
-
 Escherichia coli 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.3 DHFR
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.3 0.22
-
 7,8-dihydrofolate deletion mutant DELTAGly121/DELTAAla145, at pH 7.0 and 25°C Escherichia coli
1.5.1.3 0.37
-
 7,8-dihydrofolate deletion mutant DELTAArg52/DELTAGly121, at pH 7.0 and 25°C Escherichia coli
1.5.1.3 0.38
-
 7,8-dihydrofolate deletion mutant DELTAGly67/DELTAGly121, at pH 7.0 and 25°C Escherichia coli
1.5.1.3 1.7
-
 7,8-dihydrofolate deletion mutant DELTAGly121, at pH 7.0 and 25°C Escherichia coli
1.5.1.3 16.5
-
 7,8-dihydrofolate deletion mutant DELTAGly67, at pH 7.0 and 25°C Escherichia coli
1.5.1.3 23.1
-
 7,8-dihydrofolate deletion mutant DELTAArg52/DELTAGly67, at pH 7.0 and 25°C Escherichia coli
1.5.1.3 24
-
 7,8-dihydrofolate deletion mutant DELTAAla145, at pH 7.0 and 25°C Escherichia coli
1.5.1.3 24.8
-
 7,8-dihydrofolate wild type enzyme, at pH 7.0 and 25°C Escherichia coli
1.5.1.3 31.9
-
 7,8-dihydrofolate deletion mutant DELTAArg52/DELTAAla145, at pH 7.0 and 25°C Escherichia coli
1.5.1.3 41.1
-
 7,8-dihydrofolate deletion mutant DELTAGly67/DELTAAla145, at pH 7.0 and 25°C Escherichia coli
1.5.1.3 96.3
-
 7,8-dihydrofolate deletion mutant DELTAArg52, at pH 7.0 and 25°C Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADPH
-
 Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.5.1.3 10
-
 7,8-dihydrofolate deletion mutant DELTAArg52/DELTAGly121, at pH 7.0 and 25°C Escherichia coli
1.5.1.3 30
-
 7,8-dihydrofolate deletion mutant DELTAGly121/DELTAAla145, at pH 7.0 and 25°C Escherichia coli
1.5.1.3 70
-
 7,8-dihydrofolate deletion mutant DELTAGly67/DELTAGly121, at pH 7.0 and 25°C Escherichia coli
1.5.1.3 160
-
 7,8-dihydrofolate deletion mutant DELTAGly121, at pH 7.0 and 25°C Escherichia coli
1.5.1.3 410
-
 7,8-dihydrofolate deletion mutant DELTAArg52/DELTAAla145, at pH 7.0 and 25°C Escherichia coli
1.5.1.3 460
-
 7,8-dihydrofolate deletion mutant DELTAArg52/DELTAGly67, at pH 7.0 and 25°C Escherichia coli
1.5.1.3 830
-
 7,8-dihydrofolate deletion mutant DELTAArg52, at pH 7.0 and 25°C Escherichia coli
1.5.1.3 3300
-
 7,8-dihydrofolate deletion mutant DELTAGly67, at pH 7.0 and 25°C Escherichia coli
1.5.1.3 8600
-
 7,8-dihydrofolate deletion mutant DELTAGly67/DELTAAla145, at pH 7.0 and 25°C Escherichia coli
1.5.1.3 19200
-
 7,8-dihydrofolate wild type enzyme, at pH 7.0 and 25°C Escherichia coli
1.5.1.3 29400
-
 7,8-dihydrofolate deletion mutant DELTAAla145, at pH 7.0 and 25°C Escherichia coli