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Literature summary extracted from

  • Cooper, L.E.; Fedoseyenko, D.; Abdelwahed, S.H.; Kim, S.H.; Dairi, T.; Begley, T.P.
    In vitro reconstitution of the radical S-adenosylmethionine enzyme MqnC involved in the biosynthesis of futalosine-derived menaquinon (2013), Biochemistry, 52, 4592-4594.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.21.98.1 recombinant plasmid encoding mqnC from Bacillus halodurans C-125 is introduced into Escherichia coli BL21(DE3)-T1R containing the Escherichia coli suf operon for coexpression of His6-MqnC with iron-sulfur cluster biogenesis proteins Halalkalibacterium halodurans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.21.98.1 [4Fe-4S] cluster 4Fe-4S cluster-dependent enzyme Halalkalibacterium halodurans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.98.1 dehypoxanthine futalosine + S-adenosyl-L-methionine Halalkalibacterium halodurans
-
 cyclic dehypoxanthine futalosine + 5'-deoxyadenosine + L-methionine
-
 ?
1.21.98.1 dehypoxanthine futalosine + S-adenosyl-L-methionine Halalkalibacterium halodurans C-125
-
 cyclic dehypoxanthine futalosine + 5'-deoxyadenosine + L-methionine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.21.98.1 Halalkalibacterium halodurans Q9K864
-
-
1.21.98.1 Halalkalibacterium halodurans C-125 Q9K864
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.21.98.1 anaerobic purification by nickel affinity chromatography Halalkalibacterium halodurans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.98.1 dehypoxanthine futalosine + S-adenosyl-L-methionine
-
 Halalkalibacterium halodurans cyclic dehypoxanthine futalosine + 5'-deoxyadenosine + L-methionine
-
 ?
1.21.98.1 dehypoxanthine futalosine + S-adenosyl-L-methionine dehypoxanthine futalosine i.e. 3-[3-[(2R,3S,4R)-3,4,5-trihydroxytetrahydrofuran-2-yl]propanoyl]benzoate Halalkalibacterium halodurans cyclic dehypoxanthine futalosine + 5'-deoxyadenosine + L-methionine cyclic dehypoxanthinyl futalosine i.e. (2R,3S,4R)-3,4,5-trihydroxy-4'-oxo-3',4,4',5-tetrahydro-2'H,3H-spiro[furan-2,1'-naphthalene]-6'-carboxylate ?
1.21.98.1 dehypoxanthine futalosine + S-adenosyl-L-methionine
-
 Halalkalibacterium halodurans C-125 cyclic dehypoxanthine futalosine + 5'-deoxyadenosine + L-methionine
-
 ?
1.21.98.1 dehypoxanthine futalosine + S-adenosyl-L-methionine dehypoxanthine futalosine i.e. 3-[3-[(2R,3S,4R)-3,4,5-trihydroxytetrahydrofuran-2-yl]propanoyl]benzoate Halalkalibacterium halodurans C-125 cyclic dehypoxanthine futalosine + 5'-deoxyadenosine + L-methionine cyclic dehypoxanthinyl futalosine i.e. (2R,3S,4R)-3,4,5-trihydroxy-4'-oxo-3',4,4',5-tetrahydro-2'H,3H-spiro[furan-2,1'-naphthalene]-6'-carboxylate ?

Synonyms

EC Number Synonyms Comment Organism
1.21.98.1 dehypoxanthinyl futalosine cyclase
-
 Halalkalibacterium halodurans
1.21.98.1 DHFL cyclase
-
 Halalkalibacterium halodurans
1.21.98.1 MqnC
-
 Halalkalibacterium halodurans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.21.98.1 7.5
-
 assay at Halalkalibacterium halodurans

General Information

EC Number General Information Comment Organism
1.21.98.1 physiological function the enzyme is part of the futalosine pathway for menaquinone biosynthesis Halalkalibacterium halodurans