EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.1.14 | expressed in Escherichia coli C41(DE3) cells | Staphylococcus aureus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.8.1.14 | sitting drop vapor diffusion method, using 35-37% (w/v) PEG 600, 0.3-0.4 M MgCl2, and 0.1 M HEPES, pH 7.5 (mutants Y361F and Y419F), or 27% (w/v) PEG 600, 0.4 M MgCl2, pH 7.2 (mutant Y361F/Y419F), or 31% (w/v) PEG 600, 0.4 M MgCl2, and 0.1 M HEPES, pH 7.2 (mutant C43S) | Staphylococcus aureus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.8.1.14 | C43S | the enzyme has ca. 0.03% activity relative to the wild type enzyme | Staphylococcus aureus |
1.8.1.14 | Y361F | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Staphylococcus aureus |
1.8.1.14 | Y361F/Y419F | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Staphylococcus aureus |
1.8.1.14 | Y419F | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Staphylococcus aureus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.14 | alpha,beta-unsaturated vinyl sulfone-CoA ethyl ester | - |
Staphylococcus aureus | |
1.8.1.14 | methyl vinyl sulfone-CoA | - |
Staphylococcus aureus | |
1.8.1.14 | phenyl vinyl sulfone-CoA | - |
Staphylococcus aureus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.14 | 0.003 | - |
CoA-disulfide | wild type enzyme, at 23°C, pH not specified in the publication | Staphylococcus aureus | |
1.8.1.14 | 0.047 | - |
methyl methanethiolsulfonate | mutant enzyme Y361F, at 23°C, pH not specified in the publication | Staphylococcus aureus | |
1.8.1.14 | 0.078 | - |
methyl methanethiolsulfonate | wild type enzyme, at 23°C, pH not specified in the publication | Staphylococcus aureus | |
1.8.1.14 | 0.575 | - |
methyl methanethiolsulfonate | mutant enzyme Y419F, at 23°C, pH not specified in the publication | Staphylococcus aureus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.8.1.14 | 51000 | - |
2 * 51000, X-ray crystallography | Staphylococcus aureus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.14 | CoA-disulfide + NAD(P)H + H+ | Staphylococcus aureus | - |
CoA + NAD(P)+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.1.14 | Staphylococcus aureus | Q2FIA5 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.1.14 | ammonium sulfate precipitation adenosine 2',5'-diphosphate agarose column chromatography, and Ni-NTA column chromatography | Staphylococcus aureus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.14 | CoA-disulfide + NAD(P)H + H+ | - |
Staphylococcus aureus | CoA + NAD(P)+ | - |
? | |
1.8.1.14 | methyl methanethiolsulfonate + NAD(P)H + H+ | - |
Staphylococcus aureus | ? + NAD(P)+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.1.14 | homodimer | 2 * 51000, X-ray crystallography | Staphylococcus aureus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.1.14 | CoADR | - |
Staphylococcus aureus |
1.8.1.14 | coenzyme A-disulfide reductase | - |
Staphylococcus aureus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.14 | 1.2 | - |
methyl methanethiolsulfonate | mutant enzyme Y419F, at 23°C, pH not specified in the publication | Staphylococcus aureus | |
1.8.1.14 | 6.4 | - |
methyl methanethiolsulfonate | wild type enzyme, at 23°C, pH not specified in the publication | Staphylococcus aureus | |
1.8.1.14 | 10.4 | - |
methyl methanethiolsulfonate | mutant enzyme Y361F, at 23°C, pH not specified in the publication | Staphylococcus aureus | |
1.8.1.14 | 27 | - |
CoA-disulfide | wild type enzyme, at 23°C, pH not specified in the publication | Staphylococcus aureus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.14 | coenzyme A | - |
Staphylococcus aureus | |
1.8.1.14 | flavin | - |
Staphylococcus aureus | |
1.8.1.14 | NADH | - |
Staphylococcus aureus | |
1.8.1.14 | NADPH | - |
Staphylococcus aureus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.14 | 0.00004 | - |
phenyl vinyl sulfone-CoA | wild type enzyme, at 37°C, pH not specified in the publication | Staphylococcus aureus | |
1.8.1.14 | 0.0003 | - |
methyl vinyl sulfone-CoA | wild type enzyme, at 37°C, pH not specified in the publication | Staphylococcus aureus | |
1.8.1.14 | 0.00066 | - |
alpha,beta-unsaturated vinyl sulfone-CoA ethyl ester | wild type enzyme, at 37°C, pH not specified in the publication | Staphylococcus aureus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.14 | 2.1 | - |
methyl methanethiolsulfonate | mutant enzyme Y419F, at 23°C, pH not specified in the publication | Staphylococcus aureus | |
1.8.1.14 | 82 | - |
methyl methanethiolsulfonate | wild type enzyme, at 23°C, pH not specified in the publication | Staphylococcus aureus | |
1.8.1.14 | 220 | - |
methyl methanethiolsulfonate | mutant enzyme Y361F, at 23°C, pH not specified in the publication | Staphylococcus aureus | |
1.8.1.14 | 9000 | - |
CoA-disulfide | wild type enzyme, at 23°C, pH not specified in the publication | Staphylococcus aureus |