EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.1.3 | expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.3 | 5,6,7,8-tetrahydrofolate | non-competitive product inhibition | Mycobacterium tuberculosis | |
1.5.1.3 | NADP+ | non-competitive product inhibition | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | 0.001 | - |
NADPH | Km less than 0.001 mM, at 25°C and 100 mM HEPES, pH 7.5 | Mycobacterium tuberculosis | |
1.5.1.3 | 0.0016 | - |
7,8-dihydrofolate | with NADH as cosubstrate, at 25°C and 100 mM HEPES, pH 7.5 | Mycobacterium tuberculosis | |
1.5.1.3 | 0.067 | - |
NADPH | at 25 °C and 100 mM HEPES, pH 7.5 | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.3 | 7,8-dihydrofolate + NADH + H+ | Mycobacterium tuberculosis | - |
5,6,7,8-tetrahydrofolate + NAD+ | - |
? | |
1.5.1.3 | 7,8-dihydrofolate + NADPH + H+ | Mycobacterium tuberculosis | - |
5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.3 | Mycobacterium tuberculosis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.3 | Ni-NTA resin column chromatography | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.3 | 7,8-dihydrofolate + NADH + H+ | - |
Mycobacterium tuberculosis | 5,6,7,8-tetrahydrofolate + NAD+ | - |
? | |
1.5.1.3 | 7,8-dihydrofolate + NADPH + H+ | - |
Mycobacterium tuberculosis | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.1.3 | DHFR | - |
Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | 1.6 | - |
7,8-dihydrofolate | with NADH as cosubstrate, at 25°C and 100 mM HEPES, pH 7.5 | Mycobacterium tuberculosis | |
1.5.1.3 | 2.3 | - |
7,8-dihydrofolate | with NAPH as cosubstrate, at 25°C and 100 mM HEPES, pH 7.5 | Mycobacterium tuberculosis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.3 | NADH | - |
Mycobacterium tuberculosis | |
1.5.1.3 | NADPH | - |
Mycobacterium tuberculosis |