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Literature summary extracted from

  • Czekster, C.M.; Vandemeulebroucke, A.; Blanchard, J.S.
    Kinetic and chemical mechanism of the dihydrofolate reductase from Mycobacterium tuberculosis (2011), Biochemistry, 50, 367-375.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.3 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 5,6,7,8-tetrahydrofolate non-competitive product inhibition Mycobacterium tuberculosis
1.5.1.3 NADP+ non-competitive product inhibition Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 0.001
-
 NADPH Km less than 0.001 mM, at 25°C and 100 mM HEPES, pH 7.5 Mycobacterium tuberculosis
1.5.1.3 0.0016
-
 7,8-dihydrofolate with NADH as cosubstrate, at 25°C and 100 mM HEPES, pH 7.5 Mycobacterium tuberculosis
1.5.1.3 0.067
-
 NADPH at 25 °C and 100 mM HEPES, pH 7.5 Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADH + H+ Mycobacterium tuberculosis
-
 5,6,7,8-tetrahydrofolate + NAD+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH + H+ Mycobacterium tuberculosis
-
 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3 Ni-NTA resin column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADH + H+
-
 Mycobacterium tuberculosis 5,6,7,8-tetrahydrofolate + NAD+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH + H+
-
 Mycobacterium tuberculosis 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.3 DHFR
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.3 1.6
-
 7,8-dihydrofolate with NADH as cosubstrate, at 25°C and 100 mM HEPES, pH 7.5 Mycobacterium tuberculosis
1.5.1.3 2.3
-
 7,8-dihydrofolate with NAPH as cosubstrate, at 25°C and 100 mM HEPES, pH 7.5 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADH
-
 Mycobacterium tuberculosis
1.5.1.3 NADPH
-
 Mycobacterium tuberculosis