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Literature summary extracted from

  • Cedervall, P.E.; Dey, M.; Pearson, A.R.; Ragsdale, S.W.; Wilmot, C.M.
    Structural insight into methyl-coenzyme M reductase chemistry using coenzyme B analogues (2010), Biochemistry, 49, 7683-7693.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.8.4.1 enzyme alone and in complex with substrates, sitting drop vapor diffusion method, using 100 mM Na-HEPES, pH 7.3-8.0, 150 mM magnesium acetate, and 20-22% (w/v) PEG 400 Methanothermobacter marburgensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.4.1 O-phosphono-N-(5-sulfanylpentanoyl)-L-threonine
-
Methanothermobacter marburgensis
2.8.4.1 O-phosphono-N-(6-sulfanylhexanoyl)-L-threonine
-
Methanothermobacter marburgensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.4.1 Nickel the enzyme contains nickel Methanothermobacter marburgensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.4.1 CH3-S-CoM + HS-CoB Methanothermobacter marburgensis HS-CoB is N-(7-mercaptoheptanoyl)-L-threonine 3-O-phosphate CoM-S-S-CoB + methane
-
?
2.8.4.1 CH3-S-CoM + HS-CoB Methanothermobacter marburgensis OCM82 HS-CoB is N-(7-mercaptoheptanoyl)-L-threonine 3-O-phosphate CoM-S-S-CoB + methane
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.8.4.1 Methanothermobacter marburgensis P11558 and P11560 and P11562 subunits alpha, beta and gamma
-
2.8.4.1 Methanothermobacter marburgensis OCM82 P11558 and P11560 and P11562 subunits alpha, beta and gamma
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.4.1
-
Methanothermobacter marburgensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.4.1 CH3-S-CoM + HS-CoB HS-CoB is N-(7-mercaptoheptanoyl)-L-threonine 3-O-phosphate Methanothermobacter marburgensis CoM-S-S-CoB + methane
-
?
2.8.4.1 CH3-S-CoM + HS-CoB HS-CoB is N-(7-mercaptoheptanoyl)-L-threonine 3-O-phosphate Methanothermobacter marburgensis OCM82 CoM-S-S-CoB + methane
-
?
2.8.4.1 CH3-S-CoM + SH-CoB5 i.e. N-5-mercaptopentanoylthreonine phosphate Methanothermobacter marburgensis CoM-S-S-CoB5 + methane
-
?
2.8.4.1 CH3-S-CoM + SH-CoB5 i.e. N-5-mercaptopentanoylthreonine phosphate Methanothermobacter marburgensis OCM82 CoM-S-S-CoB5 + methane
-
?
2.8.4.1 CH3-S-CoM + SH-CoB6 i.e. N-6-mercaptohexanoylthreonine phosphate, slow substrate Methanothermobacter marburgensis CoM-S-S-CoB6 + methane
-
?
2.8.4.1 CH3-S-CoM + SH-CoB6 i.e. N-6-mercaptohexanoylthreonine phosphate, slow substrate Methanothermobacter marburgensis OCM82 CoM-S-S-CoB6 + methane
-
?
2.8.4.1 CH3-S-CoM + SH-CoB8 i.e. N-8-mercaptooctanoylthreonine phosphate Methanothermobacter marburgensis CoM-S-S-CoB8 + methane
-
?
2.8.4.1 CH3-S-CoM + SH-CoB8 i.e. N-8-mercaptooctanoylthreonine phosphate Methanothermobacter marburgensis OCM82 CoM-S-S-CoB8 + methane
-
?
2.8.4.1 CH3-S-CoM + SH-CoB9 i.e. N-9-mercaptononanoylthreonine phosphate Methanothermobacter marburgensis CoM-S-S-CoB9 + methane
-
?
2.8.4.1 CH3-S-CoM + SH-CoB9 i.e. N-9-mercaptononanoylthreonine phosphate Methanothermobacter marburgensis OCM82 CoM-S-S-CoB9 + methane
-
?

Synonyms

EC Number Synonyms Comment Organism
2.8.4.1 MCR
-
Methanothermobacter marburgensis
2.8.4.1 methyl-coenzyme M reductase
-
Methanothermobacter marburgensis

Cofactor

EC Number Cofactor Comment Organism Structure
2.8.4.1 F-430 the enzyme contains the highly reduced nickel-tetrapyrrole coenzyme F430 Methanothermobacter marburgensis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8.4.1 0.0001
-
O-phosphono-N-(6-sulfanylhexanoyl)-L-threonine pH and temperature not specified in the publication Methanothermobacter marburgensis