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Literature summary extracted from

  • Smith, M.A.; Pirrat, P.; Pearson, A.R.; Kurtis, C.R.; Trinh, C.H.; Gaule, T.G.; Knowles, P.F.; Phillips, S.E.; McPherson, M.J.
    Exploring the roles of the metal ions in Escherichia coli copper amine oxidase (2010), Biochemistry, 49, 1268-1280.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.21 expressed in Escherichia coli JM109 cells Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.21 EDTA treatment with EDTA reduces the activity of wild type enzyme Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.21 0.0012
-
 2-Phenylethylamine wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 25°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.3.21 Ca2+ calcium is the normal ligand of these peripheral sites. Enzyme activity is stimulated by 3 mM. Removal of the not solvent exposed calcium ion with EDTA results in a 60-90% reduction in enzyme activity Escherichia coli
1.4.3.21 Cu2+ the wild type enzyme contains 1 mol copper per mol of subunit, copper is the most efficient catalytic metal Escherichia coli
1.4.3.21 Mg2+ enzyme activity is stimulated by 3 mM Escherichia coli
1.4.3.21 Mn2+ enzyme activity is stimulated by 3 mM Escherichia coli
1.4.3.21 additional information not activated by Zn2+ Escherichia coli
1.4.3.21 Sr2+ enzyme activity is stimulated by 3 mM Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.21 RCH2NH2 + H2O + O2 Escherichia coli
-
 RCHO + NH3 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.21 Escherichia coli P46883
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.21 Q-Sepharose column chromatography, and gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.21 2-phenylethylamine + H2O + O2
-
 Escherichia coli 2-phenylethanal + NH3 + H2O2
-
 ?
1.4.3.21 RCH2NH2 + H2O + O2
-
 Escherichia coli RCHO + NH3 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.21 homodimer x-ray crystallography Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.4.3.21 Copper amine oxidase
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.21 12.4
-
 2-Phenylethylamine wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 25°C Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.21 2,4,5-trihydroxyphenylalanine quinone
-
 Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.3.21 10330
-
 2-Phenylethylamine wild type enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 25°C Escherichia coli