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Literature summary extracted from

  • Hu, K.; Zhao, M.; Zhang, T.; Zha, M.; Zhong, C.; Jiang, Y.; Ding, J.
    Structures of trans-2-enoyl-CoA reductases from Clostridium acetobutylicum and Treponema denticola: insights into the substrate specificity and the catalytic mechanism (2013), Biochem. J., 449, 79-89.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.44 expressed in Escherichia coli as a His-tagged fusion protein Treponema denticola
1.3.1.44 expressed in Escherichia coli as a His-tagged fusion protein Clostridium acetobutylicum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.1.44 in complex with NAD+, vapor diffusion method Clostridium acetobutylicum
1.3.1.44 in complex with NAD+, vapor diffusion method, Treponema denticola
1.3.1.44 structures of CaTER in apo form at 2.1 A resolution, in complex with NADH at 2.0 A resolution and in complex with NAD+ at 2.7 A resolution Clostridium acetobutylicum
1.3.1.44 TdTER is crystallized in complex with NAD+ at 3.0 A resolution Treponema denticola

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.44 F11K kcat slightly decreased compared to wild-type, Km (NADH) decreased compared to wild-type Clostridium acetobutylicum
1.3.1.44 F11K the mutant shows 198% activity compared to the wild type enzyme Clostridium acetobutylicum
1.3.1.44 K244A inactive Clostridium acetobutylicum
1.3.1.44 K244A mutant shows no activity Clostridium acetobutylicum
1.3.1.44 K245A kcat slightly decreased compared to wild-type, Km (NADH) increased compared to wild-type Clostridium acetobutylicum
1.3.1.44 K245A the mutant shows 67 activity compared to the wild type enzyme Clostridium acetobutylicum
1.3.1.44 Y225A kcat highly decreased compared to wild-type, Km (NADH) increased compared to wild-type Clostridium acetobutylicum
1.3.1.44 Y225A the mutant shows 6.7 activity compared to the wild type enzyme Clostridium acetobutylicum
1.3.1.44 Y235F inactive Clostridium acetobutylicum
1.3.1.44 Y235F mutant shows no activity Clostridium acetobutylicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.44 0.0316
-
 NADH pH 6.2, 25°C, mutant F11K Clostridium acetobutylicum
1.3.1.44 0.0316
-
 NADH mutant enzyme F11K, at pH 6.2 and 25°C Clostridium acetobutylicum
1.3.1.44 0.0697
-
 NADH pH 6.2, 25°C Treponema denticola
1.3.1.44 0.0697
-
 NADH wild type enzyme, at pH 6.2 and 25°C Treponema denticola
1.3.1.44 0.1054
-
 NADH pH 6.2, 25°C Clostridium acetobutylicum
1.3.1.44 0.1054
-
 NADH wild type enzyme, at pH 6.2 and 25°C Clostridium acetobutylicum
1.3.1.44 0.129
-
 NADH pH 6.2, 25°C, mutant Y225A Clostridium acetobutylicum
1.3.1.44 0.1299
-
 NADH mutant enzyme Y225A, at pH 6.2 and 25°C Clostridium acetobutylicum
1.3.1.44 0.132
-
 NADH pH 6.2, 25°C, mutant K245A Clostridium acetobutylicum
1.3.1.44 0.1323
-
 NADH mutant enzyme K245A, at pH 6.2 and 25°C Clostridium acetobutylicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.44 44800
-
-
 Treponema denticola
1.3.1.44 44800
-
 calculated from cDNA Treponema denticola
1.3.1.44 45700
-
 calculated from cDNA Clostridium acetobutylicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.9 an acyl-[acyl-carrier protein] + NAD+ Yersinia pestis
-
 a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+
-
 ?
1.3.1.44 crotonyl-CoA + NADH Clostridium acetobutylicum
-
 butanoyl-CoA + NAD+
-
 ir
1.3.1.44 crotonyl-CoA + NADH Treponema denticola
-
 butanoyl-CoA + NAD+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.9 Yersinia pestis Q8Z9U1
-
-
1.3.1.44 Clostridium acetobutylicum Q97LU2
-
-
1.3.1.44 Treponema denticola
-
-
-
1.3.1.44 Treponema denticola Q73Q47
-
-
1.3.1.44 Treponema denticola ATCC 35405
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.44 using Ni-NTA chromatography Treponema denticola
1.3.1.44 using Ni-NTA chromatography Clostridium acetobutylicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.9 an acyl-[acyl-carrier protein] + NAD+
-
 Yersinia pestis a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+
-
 ?
1.3.1.44 crotonyl-CoA + NADH
-
 Clostridium acetobutylicum butanoyl-CoA + NAD+
-
 ?
1.3.1.44 crotonyl-CoA + NADH
-
 Clostridium acetobutylicum butanoyl-CoA + NAD+
-
 ir
1.3.1.44 crotonyl-CoA + NADH
-
 Treponema denticola butanoyl-CoA + NAD+
-
 ir
1.3.1.44 crotonyl-CoA + NADH high activity toward crotonyl-CoA Treponema denticola butanoyl-CoA + NAD+
-
 ?
1.3.1.44 crotonyl-CoA + NADH high activity toward crotonyl-CoA Treponema denticola ATCC 35405 butanoyl-CoA + NAD+
-
 ?
1.3.1.44 crotonyl-CoA + NADPH
-
 Clostridium acetobutylicum butanoyl-CoA + NADP+
-
 ?
1.3.1.44 hexenoyl-CoA + NADH weak activity toward crotonyl-CoA Treponema denticola ? + NAD+
-
 ?
1.3.1.44 hexenoyl-CoA + NADH weak activity toward crotonyl-CoA Treponema denticola ATCC 35405 ? + NAD+
-
 ?
1.3.1.44 additional information no activity with butanoyl-CoA Clostridium acetobutylicum ?
-
 ?
1.3.1.44 additional information no activity with butanoyl-CoA Treponema denticola ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.44 ? x * 45700, calculated from amino acid sequence Clostridium acetobutylicum
1.3.1.44 ? x * 44800, calculated from amino acid sequence Treponema denticola
1.3.1.44 monomer
-
 Treponema denticola
1.3.1.44 monomer
-
 Clostridium acetobutylicum

Synonyms

EC Number Synonyms Comment Organism
1.3.1.9 FabV
-
 Yersinia pestis
1.3.1.44 CaTER
-
 Clostridium acetobutylicum
1.3.1.44 tdTer
-
 Treponema denticola
1.3.1.44 TER
-
 Clostridium acetobutylicum
1.3.1.44 TER
-
 Treponema denticola
1.3.1.44 trans-2-enoyl-CoA reductase
-
 Clostridium acetobutylicum
1.3.1.44 trans-2-enoyl-CoA reductase
-
 Treponema denticola
1.3.1.44 trans-2-enoyl-CoA reductases
-
 Treponema denticola
1.3.1.44 trans-2-enoyl-CoA reductases
-
 Clostridium acetobutylicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.44 25
-
 assay at Treponema denticola
1.3.1.44 25
-
 assay at Clostridium acetobutylicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.44 2.3
-
 NADH pH 6.2, 25°C, mutant Y225A Clostridium acetobutylicum
1.3.1.44 2.3
-
 NADH mutant enzyme Y225A, at pH 6.2 and 25°C Clostridium acetobutylicum
1.3.1.44 16.5
-
 NADH pH 6.2, 25°C, mutant F11K Clostridium acetobutylicum
1.3.1.44 16.5
-
 NADH mutant enzyme F11K, at pH 6.2 and 25°C Clostridium acetobutylicum
1.3.1.44 23.6
-
 NADH pH 6.2, 25°C, mutant K245A Clostridium acetobutylicum
1.3.1.44 23.6
-
 NADH mutant enzyme K245A, at pH 6.2 and 25°C Clostridium acetobutylicum
1.3.1.44 28.2
-
 NADH pH 6.2, 25°C, wild-type Clostridium acetobutylicum
1.3.1.44 28.2
-
 NADH wild type enzyme, at pH 6.2 and 25°C Clostridium acetobutylicum
1.3.1.44 385.9
-
 NADH pH 6.2, 25°C, wild-type Treponema denticola
1.3.1.44 385.9
-
 NADH wild type enzyme, at pH 6.2 and 25°C Treponema denticola

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.44 6.2
-
 assay at Treponema denticola
1.3.1.44 6.2
-
 assay at Clostridium acetobutylicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.9 NAD+
-
 Yersinia pestis
1.3.1.9 NADH
-
 Yersinia pestis
1.3.1.44 NADH
-
 Clostridium acetobutylicum
1.3.1.44 NADH
-
 Treponema denticola
1.3.1.44 NADH higher activity toward NADH than NADPH Treponema denticola
1.3.1.44 NADH higher activity toward NADH than NADPH Clostridium acetobutylicum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3.1.44 18
-
 NADH pH 6.2, 25°C, mutant Y225A Clostridium acetobutylicum
1.3.1.44 18
-
 NADH mutant enzyme Y225A, at pH 6.2 and 25°C Clostridium acetobutylicum
1.3.1.44 180
-
 NADH pH 6.2, 25°C, mutant K245A Clostridium acetobutylicum
1.3.1.44 180
-
 NADH mutant enzyme K245A, at pH 6.2 and 25°C Clostridium acetobutylicum
1.3.1.44 270
-
 NADH pH 6.2, 25°C, wild-type Clostridium acetobutylicum
1.3.1.44 270
-
 NADH wild type enzyme, at pH 6.2 and 25°C Clostridium acetobutylicum
1.3.1.44 520
-
 NADH pH 6.2, 25°C, mutant F11K Clostridium acetobutylicum
1.3.1.44 520
-
 NADH mutant enzyme F11K, at pH 6.2 and 25°C Clostridium acetobutylicum
1.3.1.44 5500
-
 NADH pH 6.2, 25°C, wild-type Treponema denticola
1.3.1.44 5500
-
 NADH wild type enzyme, at pH 6.2 and 25°C Treponema denticola