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Literature summary extracted from

  • Wojcieszynska, D.; Hupert-Kocurek, K.; Jankowska, A.; Guzik, U.
    Properties of catechol 2,3-dioxygenase from crude extract of Stenotrophomonas maltophilia strain KB2 immobilized in calcium alginate hydrogels (2012), Biochem. Eng. J., 66, 1-7.
No PubMed abstract available

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.2 additional information immobilization of catechol 2,3-dioxygenase from KB2 strain using calcium alginate, the iimmobilized enzyme shows relatively higher activity against 3-methylcatechol, 4-methylcatechol, 4,5-dichlorocatechol, 3,5-dichlorocatechol, hydroquinone and tetrachlorohydroquinone than soluble enzyme, immobilization protects the enzyme from the inhibition and enhanced its resistance to inactivation during catalysis Stenotrophomonas maltophilia

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.2 CuSO4 the immobilized enzyme is less sensitive compared to the free enzyme Stenotrophomonas maltophilia
1.13.11.2 H2O2 the immobilized enzyme is less sensitive compared to the free enzyme Stenotrophomonas maltophilia
1.13.11.2 NaN3 the immobilized enzyme is less sensitive compared to the free enzyme Stenotrophomonas maltophilia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.2 0.0025
-
 4-methylcatechol pH 8.0, 35°C, immobilized enzyme Stenotrophomonas maltophilia
1.13.11.2 0.0425
-
 4-methylcatechol pH 8.0, 35°C, free enzyme Stenotrophomonas maltophilia

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.2 Stenotrophomonas maltophilia
-
-
-
1.13.11.2 Stenotrophomonas maltophilia KB2
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.2 355.8
-
 substrate 4-methylcatechol, pH 8.0, 35°C, free enzyme Stenotrophomonas maltophilia
1.13.11.2 936.3
-
 substrate 4-methylcatechol, pH 8.0, 35°C, immobilized enzyme Stenotrophomonas maltophilia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.2 2,4-dichlorophenol + O2
-
 Stenotrophomonas maltophilia ?
-
 ?
1.13.11.2 2,4-dichlorophenol + O2
-
 Stenotrophomonas maltophilia KB2 ?
-
 ?
1.13.11.2 2-chlorophenol + O2
-
 Stenotrophomonas maltophilia ?
-
 ?
1.13.11.2 2-chlorophenol + O2
-
 Stenotrophomonas maltophilia KB2 ?
-
 ?
1.13.11.2 2-methylphenol + O2
-
 Stenotrophomonas maltophilia ?
-
 ?
1.13.11.2 3,5-dichlorocatechol + O2 immobilized enzyme, no activity with the free enzyme Stenotrophomonas maltophilia ?
-
 ?
1.13.11.2 3,5-dichlorocatechol + O2 immobilized enzyme, no activity with the free enzyme Stenotrophomonas maltophilia KB2 ?
-
 ?
1.13.11.2 3-methylcatechol + O2
-
 Stenotrophomonas maltophilia ?
-
 ?
1.13.11.2 3-methylcatechol + O2
-
 Stenotrophomonas maltophilia KB2 ?
-
 ?
1.13.11.2 3-methylphenol + O2
-
 Stenotrophomonas maltophilia ?
-
 ?
1.13.11.2 4,5-dichlorocatechol + O2
-
 Stenotrophomonas maltophilia ?
-
 ?
1.13.11.2 4-chlorophenol + O2
-
 Stenotrophomonas maltophilia ?
-
 ?
1.13.11.2 4-methylcatechol + O2
-
 Stenotrophomonas maltophilia ?
-
 ?
1.13.11.2 butanol + O2
-
 Stenotrophomonas maltophilia ?
-
 ?
1.13.11.2 catechol + O2
-
 Stenotrophomonas maltophilia 2-hydroxymuconate-6-semialdehyde
-
 ?
1.13.11.2 ethanol + O2
-
 Stenotrophomonas maltophilia ?
-
 ?
1.13.11.2 hydroquinone + O2 immobilized enzyme, no activity with the free enzyme Stenotrophomonas maltophilia ?
-
 ?
1.13.11.2 hydroquinone + O2 immobilized enzyme, no activity with the free enzyme Stenotrophomonas maltophilia KB2 ?
-
 ?
1.13.11.2 methanol + O2
-
 Stenotrophomonas maltophilia ?
-
 ?
1.13.11.2 propanol + O2
-
 Stenotrophomonas maltophilia ?
-
 ?
1.13.11.2 tetrachlorohydroquinone + O2 immobilized enzyme, no activity with the free enzyme Stenotrophomonas maltophilia ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.2 35
-
 assay at Stenotrophomonas maltophilia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.2 8
-
 both free and the immobilized enzyme Stenotrophomonas maltophilia

General Information

EC Number General Information Comment Organism
1.13.11.2 additional information there is a hydrophobic channel in the active site of catechol 2,3-dioxygenase. Through this channel catechol penetrates into the active site of the enzyme Stenotrophomonas maltophilia