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Literature summary extracted from

  • Li, J.; Gan, J.H.; Mathews, F.S.; Xia, Z.X.
    The enzymatic reaction-induced configuration change of the prosthetic group PQQ of methanol dehydrogenase (2011), Biochem. Biophys. Res. Commun., 406, 621-626.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.2.7 purified enzyme, 5 mg/mL protein in 50 mM Tris-HCl, pH 8.25, and 13.5% PEG 8000, mixed with crystallization solution containing 1 and 50 mM methanol resulting in crystal forms A, B or C with or without incorporated methanol or ethanool, 20°C, X--ray diffraction structure determination and analysis at 1.5-3.0 A resolution, molecular modeling Methylophilus methylotrophus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.2.7 Ca2+ two Ca2+ ions per enzyme tetramer, essential for the enzymatic activity Methylophilus methylotrophus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.2.7 a primary alcohol + 2 ferricytochrome cL Methylophilus methylotrophus
-
 an aldehyde + 2 ferrocytochrome cL + 2 H+
-
 ?
1.1.2.7 a primary alcohol + 2 ferricytochrome cL Methylophilus methylotrophus W3A1
-
 an aldehyde + 2 ferrocytochrome cL + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.7 Methylophilus methylotrophus P38539
-
-
1.1.2.7 Methylophilus methylotrophus W3A1 P38539
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.2.7 a primary alcohol + 2 ferricytochrome cL = an aldehyde + 2 ferrocytochrome cL + 2 H+ chemical structure of pyrroloquinoline quinone and hydride transfer mechanism of the enzymatic reaction catalyzed by MEDH, overview Methylophilus methylotrophus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.7 a primary alcohol + 2 ferricytochrome cL
-
 Methylophilus methylotrophus an aldehyde + 2 ferrocytochrome cL + 2 H+
-
 ?
1.1.2.7 a primary alcohol + 2 ferricytochrome cL
-
 Methylophilus methylotrophus W3A1 an aldehyde + 2 ferrocytochrome cL + 2 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.2.7 heterotetramer alpha2beta2, the MEDH heterotetramer is composed of two large subunits and two small subunits Methylophilus methylotrophus
1.1.2.7 More three-dimensional structrue determination of MEDH with bound methanol or ethanol, overview Methylophilus methylotrophus

Synonyms

EC Number Synonyms Comment Organism
1.1.2.7 MEDH
-
 Methylophilus methylotrophus
1.1.2.7 methanol dehydrogenase
-
 Methylophilus methylotrophus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.7 pyrroloquinoline quinone the enzyme contains the prosthetic group pyrroloquinoline quinone, PQQ, non-covalently bound, which catalyzes the oxidation of methanol to formaldehyde, two molecules per enzyme tetramer, chemical structure and configuration change of PQQ., overview Methylophilus methylotrophus

General Information

EC Number General Information Comment Organism
1.1.2.7 additional information the enzyme contains the prosthetic group pyrroloquinoline quinone, PQQ, which catalyzes the oxidation of methanol to formaldehyde, active site structure with pyrroloquinoline quinone and Ca2+, substrate binding structure, overview Methylophilus methylotrophus