EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.1.3 | expressed in Escherichia coli JM109 cells | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.5.1.3 | R44A | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
1.5.1.3 | R44C | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
1.5.1.3 | R44D | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
1.5.1.3 | R44E | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
1.5.1.3 | R44F | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
1.5.1.3 | R44G | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
1.5.1.3 | R44I | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
1.5.1.3 | R44K | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
1.5.1.3 | R44L | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
1.5.1.3 | R44M | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
1.5.1.3 | R44N | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
1.5.1.3 | R44P | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
1.5.1.3 | R44Q | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
1.5.1.3 | R44S | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
1.5.1.3 | R44T | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
1.5.1.3 | R44V | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
1.5.1.3 | R44W | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
1.5.1.3 | R44Y | the mutation significantly reduces the enzymatic activity and the binding affinity toward the cofactor NADPH | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | 0.0007 | - |
7,8-dihydrofolate | mutant enzyme R44H, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 0.0008 | - |
7,8-dihydrofolate | mutant enzyme R44V, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 0.001 | - |
7,8-dihydrofolate | mutant enzyme R44Y, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 0.0011 | - |
7,8-dihydrofolate | mutant enzyme R44C, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 0.0014 | - |
7,8-dihydrofolate | mutant enzyme R44L, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 0.0015 | - |
7,8-dihydrofolate | mutant enzyme R44G, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 0.0016 | - |
7,8-dihydrofolate | mutant enzyme R44A, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 0.0016 | - |
7,8-dihydrofolate | wild type enzyme, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 0.0019 | - |
7,8-dihydrofolate | mutant enzyme R44E, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 0.0021 | - |
7,8-dihydrofolate | mutant enzyme R44I, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 0.0023 | - |
7,8-dihydrofolate | mutant enzyme R44M, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 0.0024 | - |
7,8-dihydrofolate | mutant enzyme R44Q, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 0.0025 | - |
7,8-dihydrofolate | mutant enzyme R44K, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 0.0025 | - |
7,8-dihydrofolate | mutant enzyme R44N, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 0.0025 | - |
7,8-dihydrofolate | mutant enzyme R44T, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 0.0027 | - |
7,8-dihydrofolate | mutant enzyme R44F, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 0.0029 | - |
7,8-dihydrofolate | mutant enzyme R44P, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 0.003 | - |
7,8-dihydrofolate | mutant enzyme R44D, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 0.0031 | - |
7,8-dihydrofolate | mutant enzyme R44S, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 0.0037 | - |
7,8-dihydrofolate | mutant enzyme R44W, at 15°C, pH not specified in the publication | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.3 | 7,8-dihydrofolate + NADPH + H+ | Escherichia coli | - |
5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.3 | Escherichia coli | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.3 | 7,8-dihydrofolate + NADPH + H+ | - |
Escherichia coli | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.1.3 | DHFR | - |
Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | 0.3 | - |
7,8-dihydrofolate | mutant enzyme R44P, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 0.69 | - |
7,8-dihydrofolate | mutant enzyme R44D, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 0.89 | - |
7,8-dihydrofolate | mutant enzyme R44E, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 0.89 | - |
7,8-dihydrofolate | mutant enzyme R44Y, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 0.98 | - |
7,8-dihydrofolate | mutant enzyme R44L, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 1.15 | - |
7,8-dihydrofolate | mutant enzyme R44I, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 1.31 | - |
7,8-dihydrofolate | mutant enzyme R44V, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 1.39 | - |
7,8-dihydrofolate | mutant enzyme R44F, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 1.59 | - |
7,8-dihydrofolate | mutant enzyme R44H, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 1.78 | - |
7,8-dihydrofolate | mutant enzyme R44G, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 1.9 | - |
7,8-dihydrofolate | mutant enzyme R44Q, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 2 | - |
7,8-dihydrofolate | mutant enzyme R44T, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 2.41 | - |
7,8-dihydrofolate | mutant enzyme R44W, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 2.43 | - |
7,8-dihydrofolate | mutant enzyme R44C, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 3.05 | - |
7,8-dihydrofolate | mutant enzyme R44A, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 3.3 | - |
7,8-dihydrofolate | mutant enzyme R44N, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 3.8 | - |
7,8-dihydrofolate | mutant enzyme R44M, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 5.4 | - |
7,8-dihydrofolate | wild type enzyme, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 5.7 | - |
7,8-dihydrofolate | mutant enzyme R44K, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 5.8 | - |
7,8-dihydrofolate | mutant enzyme R44S, at 15°C, pH not specified in the publication | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.3 | NADPH | - |
Escherichia coli |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | 100 | - |
7,8-dihydrofolate | mutant enzyme R44P, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 230 | - |
7,8-dihydrofolate | mutant enzyme R44D, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 470 | - |
7,8-dihydrofolate | mutant enzyme R44E, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 500 | - |
7,8-dihydrofolate | mutant enzyme R44F, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 560 | - |
7,8-dihydrofolate | mutant enzyme R44I, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 660 | - |
7,8-dihydrofolate | mutant enzyme R44W, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 680 | - |
7,8-dihydrofolate | mutant enzyme R44L, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 800 | - |
7,8-dihydrofolate | mutant enzyme R44Q, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 800 | - |
7,8-dihydrofolate | mutant enzyme R44T, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 900 | - |
7,8-dihydrofolate | mutant enzyme R44Y, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 1200 | - |
7,8-dihydrofolate | mutant enzyme R44G, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 1300 | - |
7,8-dihydrofolate | mutant enzyme R44N, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 1600 | - |
7,8-dihydrofolate | mutant enzyme R44M, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 1700 | - |
7,8-dihydrofolate | mutant enzyme R44V, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 1900 | - |
7,8-dihydrofolate | mutant enzyme R44A, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 1900 | - |
7,8-dihydrofolate | mutant enzyme R44S, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 2200 | - |
7,8-dihydrofolate | mutant enzyme R44C, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 2300 | - |
7,8-dihydrofolate | mutant enzyme R44K, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 2500 | - |
7,8-dihydrofolate | mutant enzyme R44H, at 15°C, pH not specified in the publication | Escherichia coli | |
1.5.1.3 | 3400 | - |
7,8-dihydrofolate | wild type enzyme, at 15°C, pH not specified in the publication | Escherichia coli |