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Literature summary extracted from

  • Shrivastava, R.; Basu, A.; Phale, P.S.
    Purification and characterization of benzyl alcohol- and benzaldehyde-dehydrogenase from Pseudomonas putida CSV86 (2011), Arch. Microbiol., 193, 553-563.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.2.1.28 the enzyme loses its activity irreversibly when dialyzed against 50 mM potassium phosphate, pH 6.5, 5% (v/v) glycerol, 2 mM dithiothreitol without benzyl alcohol for 5 h. Enzyme activity is unaffected by varying the concentration of benzyl alcohol Pseudomonas putida
1.2.1.28 the presence of magnesium may be essential for the stability of the enzyme Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.28 1,10-phenanthroline inhibits BZDH by 60%, probably by chelation of tightly bound Mg2+ ions Pseudomonas putida
1.2.1.28 2,2'-dipyridyl 40% residual activity at 1 mM; inhibits BZDH by 60%, probably by chelation of tightly bound Mg2+ ions Pseudomonas putida
1.2.1.28 2-Hydroxybenzaldehyde substrate inhibition Pseudomonas putida
1.2.1.28 2-Methylbenzaldehyde substrate inhibition Pseudomonas putida
1.2.1.28 2-naphthaldehyde substrate inhibition Pseudomonas putida
1.2.1.28 3-Chlorobenzaldehyde substrate inhibition Pseudomonas putida
1.2.1.28 3-Hydroxybenzaldehyde substrate inhibition Pseudomonas putida
1.2.1.28 3-Methoxybenzaldehyde substrate inhibition Pseudomonas putida
1.2.1.28 3-methylbenzaldehyde substrate inhibition Pseudomonas putida
1.2.1.28 4-Chlorobenzaldehyde substrate inhibition Pseudomonas putida
1.2.1.28 4-methoxybenzaldehyde substrate inhibition Pseudomonas putida
1.2.1.28 4-methylbenzaldehyde substrate inhibition Pseudomonas putida
1.2.1.28 benzaldehyde substrate inhibition; substrate inhibition at higher concentration of benzaldehyde Pseudomonas putida
1.2.1.28 additional information metal chelators (10 mM) like EDTA and EGTA do not affect the activity Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.28 0.0014
-
benzaldehyde apparent Km-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.0014
-
benzaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.0018
-
3-methylbenzaldehyde apparent Km-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.0018
-
3-methylbenzaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.0028
-
3-Hydroxybenzaldehyde apparent Km-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.0028
-
3-Chlorobenzaldehyde apparent Km-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.0028
-
3-Hydroxybenzaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.0028
-
3-Chlorobenzaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.003
-
4-Chlorobenzaldehyde apparent Km-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.003
-
4-Chlorobenzaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.0035
-
4-methoxybenzaldehyde apparent Km-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.0035
-
4-methoxybenzaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.0038
-
2-naphthaldehyde apparent Km-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.0038
-
2-naphthaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.02
-
4-methylbenzaldehyde apparent Km-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.02
-
4-methylbenzaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.115
-
NAD+ apparent Km-value at substrate saturation, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.129
-
3-Methoxybenzaldehyde apparent Km-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.129
-
3-Methoxybenzaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.377
-
2-Methylbenzaldehyde apparent Km-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.377
-
2-Methylbenzaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.497
-
2-Hydroxybenzaldehyde apparent Km-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.497
-
2-Hydroxybenzaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.28 Mg2+ 5.1 mol Mg2+ per mol enzyme Pseudomonas putida
1.2.1.28 Mg2+ tightly bound Pseudomonas putida
1.2.1.28 additional information BZDH shows the presence of 5.1 mol of magnesium per mole of the enzyme Pseudomonas putida
1.2.1.28 additional information external addition of Cu2+, Fe3+, Ca2+, Mg2+, Mn2+, and Zn2+ up to 2.5 mM in the assay mixture do not affect activity Pseudomonas putida
1.2.1.28 additional information external addition of Cu2+, Fe3+, Ca2+, Mg2+, Mn2+, or Zn2+ (up to 2.5 mM) does not affect the enzymatic activity Pseudomonas putida
1.2.1.28 additional information metal chelators at 10 mM like EDTA and EGTA do not affect activity Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.28 57000
-
subunit molecular mass, determined by SDS–PAGE Pseudomonas putida
1.2.1.28 117000
-
gel filtration Pseudomonas putida
1.2.1.28 117000
-
native molecular mass of BZDH, determined by Sephacryl S-300-HR gel filtration chromatography Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.28 benzaldehyde + NAD+ + H2O Pseudomonas putida
-
benzoate + NADH + H+
-
?
1.2.1.28 benzaldehyde + NAD+ + H2O Pseudomonas putida CSV86
-
benzoate + NADH + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.28 Pseudomonas putida
-
-
-
1.2.1.28 Pseudomonas putida
-
MTCC2445
-
1.2.1.28 Pseudomonas putida CSV86
-
-
-
1.2.1.28 Pseudomonas putida CSV86
-
MTCC2445
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.28 ammonium sulfate precipitation, phenyl Sepharose column chromatography, DEAE Sephacel column chromatography, Blue agarose column chromatography, Q Sepharose column chromatography, and Sephacryl S-300-HR gel filtration Pseudomonas putida
1.2.1.28 to apparent homogeneity by ammonium sulfate precipitation and DEAE Sephacel chromatography and resolved by Blue agarose matrix, where BZDH binds to the matrix, BZDH further purified by Sephacryl S-300-HR gel filtration chromatography Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.28 0.32
-
cell free extract, in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 64
-
after 200fold purification, in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.2.1.28 purified enzyme preparations are found to be stable and stored either at 4 or -20°C, stable up to 6 months at -20°C Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.28 2-hydroxybenzaldehyde + NAD+ + H2O
-
Pseudomonas putida 2-hydroxybenzoate + NADH + H+
-
?
1.2.1.28 2-hydroxybenzaldehyde + NAD+ + H2O
-
Pseudomonas putida CSV86 2-hydroxybenzoate + NADH + H+
-
?
1.2.1.28 2-hydroxybenzaldehyde + NAD+ + H2O
-
Pseudomonas putida 2-hydroxybenzoate + NADH + 2 H+
-
?
1.2.1.28 2-methylbenzaldehyde + NAD+ + H2O
-
Pseudomonas putida 2-methylbenzoate + NADH + 2 H+
-
?
1.2.1.28 2-methylbenzaldehyde + NAD+ + H2O
-
Pseudomonas putida 2-methylbenzoate + NADH + H+
-
?
1.2.1.28 2-naphthaldehyde + NAD+ + H2O
-
Pseudomonas putida 2-naphthoate + NADH + H+
-
?
1.2.1.28 2-naphthaldehyde + NAD+ + H2O
-
Pseudomonas putida naphthalene-2-carboxylic acid + NADH + 2 H+
-
?
1.2.1.28 3-chlorobenzaldehyde + NAD+ + H2O
-
Pseudomonas putida 3-chlorobenzoate + NADH + 2 H+
-
?
1.2.1.28 3-chlorobenzaldehyde + NAD+ + H2O
-
Pseudomonas putida 3-chlorobenzoate + NADH + H+
-
?
1.2.1.28 3-hydroxybenzaldehyde + NAD+ + H2O
-
Pseudomonas putida 3-hydroxybenzoate + NADH + H+
-
?
1.2.1.28 3-hydroxybenzaldehyde + NAD+ + H2O
-
Pseudomonas putida CSV86 3-hydroxybenzoate + NADH + H+
-
?
1.2.1.28 3-hydroxybenzaldehyde + NAD+ + H2O
-
Pseudomonas putida 3-hydroxybenzoate + NADH + 2 H+
-
?
1.2.1.28 3-methoxybenzaldehyde + NAD+ + H2O
-
Pseudomonas putida 3-methoxybenzoate + NADH + H+
-
?
1.2.1.28 3-methoxybenzaldehyde + NAD+ + H2O
-
Pseudomonas putida 3-methoxybenzoate + NADH + 2 H+
-
?
1.2.1.28 3-methylbenzaldehyde + NAD+ + H2O
-
Pseudomonas putida 3-methylbenzoate + NADH + H+
-
?
1.2.1.28 3-methylbenzaldehyde + NAD+ + H2O
-
Pseudomonas putida 3-methylbenzoate + NADH + 2 H+
-
?
1.2.1.28 4-chlorobenzaldehyde + NAD+ + H2O
-
Pseudomonas putida 4-chlorobenzoate + NADH + 2 H+
-
?
1.2.1.28 4-chlorobenzaldehyde + NAD+ + H2O
-
Pseudomonas putida 4-chlorobenzoate + NADH + H+
-
?
1.2.1.28 4-methoxybenzaldehyde + NAD+ + H2O
-
Pseudomonas putida 4-methoxybenzoate + NADH + H+
-
?
1.2.1.28 4-methoxybenzaldehyde + NAD+ + H2O
-
Pseudomonas putida 4-methoxybenzoate + NADH + 2 H+
-
?
1.2.1.28 4-methylbenzaldehyde + NAD+ + H2O
-
Pseudomonas putida 4-methylbenzoate + NADH + H+
-
?
1.2.1.28 4-methylbenzaldehyde + NAD+ + H2O
-
Pseudomonas putida 4-methylbenzoate + NADH + 2 H+
-
?
1.2.1.28 benzaldehyde + NAD+ + H2O
-
Pseudomonas putida benzoate + NADH + H+
-
?
1.2.1.28 benzaldehyde + NAD+ + H2O
-
Pseudomonas putida CSV86 benzoate + NADH + H+
-
?
1.2.1.28 benzaldehyde + NAD+ + H2O
-
Pseudomonas putida benzoate + NADH + 2 H+
-
ir
1.2.1.28 additional information enzyme has a broad substrate specificity accepting mono- and di-aromatic aldehydes but not 2-methoxy derivatives or aliphatic primary aldehydes with 1-6 carbon atoms, no activity with NADP+ or NADPH, BZDH prefers benzaldehydes having substitution either at meta- or para-positions Pseudomonas putida ?
-
?
1.2.1.28 additional information the enzyme fails to show activity with NADP+ or NADPH and is inactive with 2-methoxybenzaldehyde, 4-hydroxybenzaldehyde, 2-chlorobenzaldehyde, and 1-naphthaldehyde Pseudomonas putida ?
-
?
1.2.1.28 additional information enzyme has a broad substrate specificity accepting mono- and di-aromatic aldehydes but not 2-methoxy derivatives or aliphatic primary aldehydes with 1-6 carbon atoms, no activity with NADP+ or NADPH, BZDH prefers benzaldehydes having substitution either at meta- or para-positions Pseudomonas putida CSV86 ?
-
?
1.2.1.28 additional information the enzyme fails to show activity with NADP+ or NADPH and is inactive with 2-methoxybenzaldehyde, 4-hydroxybenzaldehyde, 2-chlorobenzaldehyde, and 1-naphthaldehyde Pseudomonas putida CSV86 ?
-
?

Subunits

EC Number Subunits Comment Organism
1.2.1.28 homodimer 2 * 57000, SDS-PAGE Pseudomonas putida
1.2.1.28 homodimer 2 * 57000 Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.2.1.28 benzaldehyde dehydrogenase
-
Pseudomonas putida
1.2.1.28 BZDH
-
Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.28 14
-
benzaldehyde apparent Kcat-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 14
-
benzaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 16
-
3-Chlorobenzaldehyde apparent Kcat-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 16
-
3-Chlorobenzaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 23
-
3-methylbenzaldehyde apparent Kcat-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 23
-
3-methylbenzaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 24
-
3-Hydroxybenzaldehyde apparent Kcat-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 24
-
3-Hydroxybenzaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 33
-
2-Hydroxybenzaldehyde apparent Kcat-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 33
-
2-Hydroxybenzaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 39
-
2-Methylbenzaldehyde apparent Kcat-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 39
-
2-Methylbenzaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 56
-
2-naphthaldehyde apparent Kcat-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 56
-
2-naphthaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 67
-
4-Chlorobenzaldehyde apparent Kcat-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 67
-
4-Chlorobenzaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 101
-
4-methoxybenzaldehyde apparent Kcat-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 101
-
4-methoxybenzaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 120
-
4-methylbenzaldehyde apparent Kcat-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 120
-
4-methylbenzaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 885
-
3-Methoxybenzaldehyde apparent Kcat-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 885
-
3-Methoxybenzaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.28 9.6
-
-
Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.28 additional information not active with NADP+ Pseudomonas putida
1.2.1.28 NAD+
-
Pseudomonas putida
1.2.1.28 NAD+ dependent on Pseudomonas putida

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.28 0.0013
-
3-Methoxybenzaldehyde apparent Ki-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.003
-
4-methylbenzaldehyde apparent Ki-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.006
-
benzaldehyde apparent Ki-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.006
-
benzaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.008
-
2-naphthaldehyde apparent Ki-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.013
-
4-Chlorobenzaldehyde apparent Ki-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.035
-
4-methoxybenzaldehyde apparent Ki-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.051
-
3-methylbenzaldehyde apparent Ki-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.068
-
3-Chlorobenzaldehyde apparent Ki-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.131
-
3-Hydroxybenzaldehyde apparent Ki-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 0.521
-
2-Methylbenzaldehyde apparent Ki-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 1.808
-
2-Hydroxybenzaldehyde apparent Ki-value, pH 9.6, temperature not specified in the publication Pseudomonas putida

General Information

EC Number General Information Comment Organism
1.2.1.28 metabolism the wide substrate specificity of the enzyme enables the strain to detoxify methylnaphthalenes to naphthoic acids efficiently Pseudomonas putida

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.1.28 60
-
2-Hydroxybenzaldehyde apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 60
-
2-Hydroxybenzaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 110
-
2-Methylbenzaldehyde apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 110
-
2-Methylbenzaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 5700
-
3-Chlorobenzaldehyde apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 5700
-
3-Chlorobenzaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 6000
-
4-methylbenzaldehyde apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 6000
-
4-methylbenzaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 6800
-
3-Methoxybenzaldehyde apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 6800
-
3-Methoxybenzaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 9000
-
3-Hydroxybenzaldehyde apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 9000
-
3-Hydroxybenzaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 10000
-
benzaldehyde apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 10000
-
benzaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 13000
-
3-methylbenzaldehyde apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 13000
-
3-methylbenzaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 14700
-
2-naphthaldehyde apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 14700
-
2-naphthaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 22000
-
4-Chlorobenzaldehyde apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 22000
-
4-Chlorobenzaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida
1.2.1.28 29000
-
4-methoxybenzaldehyde apparent Kcat/Km-value, pH 9.6, temperature not specified in the publication Pseudomonas putida
1.2.1.28 29000
-
4-methoxybenzaldehyde in glycine-KOH buffer (100 mM, pH 9.6), temperature not specified in the publication Pseudomonas putida