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Literature summary extracted from

  • Di Gennaro, P.; Bargna, A.; Bruno, F.; Sello, G.
    Purification of recombinant catalase-peroxidase HPI from E. coli and its application in enzymatic polymerization reactions (2014), Appl. Microbiol. Biotechnol., 98, 1119-1126.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.21 expression in Escherichia coli Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.21 Escherichia coli B1IVD5
-
-
1.11.1.21 Escherichia coli DSM 1576 B1IVD5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.21 recombinant protein Escherichia coli

Storage Stability

EC Number Storage Stability Organism
1.11.1.21 -20°C, 10 mM phosphate buffer, loss of activtiy Escherichia coli
1.11.1.21 -20°C, Tris-HCl buffer, stable Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.21 3-methoxyphenol + H2O2 about 90% conversion Escherichia coli ? + H2O products are oligomers of different weight, i.g. of 27 monomer units and of 23 monomer units ?
1.11.1.21 3-methoxyphenol + H2O2 about 90% conversion Escherichia coli DSM 1576 ? + H2O products are oligomers of different weight, i.g. of 27 monomer units and of 23 monomer units ?
1.11.1.21 aniline + H2O2 about 25% conversion Escherichia coli ? + H2O
-
 ?
1.11.1.21 aniline + H2O2 about 25% conversion Escherichia coli DSM 1576 ? + H2O
-
 ?
1.11.1.21 catechol + H2O2 about 90% conversion Escherichia coli ? + H2O products are oligomers of 7 monomer units ?
1.11.1.21 catechol + H2O2 about 90% conversion Escherichia coli DSM 1576 ? + H2O products are oligomers of 7 monomer units ?
1.11.1.21 o-dianisidine + H2O2
-
 Escherichia coli ? + H2O
-
 ?
1.11.1.21 o-dianisidine + H2O2
-
 Escherichia coli DSM 1576 ? + H2O
-
 ?
1.11.1.21 phenol + H2O2 about 90% conversion Escherichia coli ? + H2O products are oligomers of different weight, i.g. of 27 monomer units and of 23 monomer units ?
1.11.1.21 phenol + H2O2 about 90% conversion Escherichia coli DSM 1576 ? + H2O products are oligomers of different weight, i.g. of 27 monomer units and of 23 monomer units ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.21 HPI
-
 Escherichia coli
1.11.1.21 KatG
-
 Escherichia coli

Expression

EC Number Organism Comment Expression
1.11.1.21 Escherichia coli addition of 0.5 mM Fe(II)-citrate to culture liquid results in 36% increase in enzyme, substrate o-dianisidine up