Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kubota, T.; Tanaka, Y.; Hiraga, K.; Inui, M.; Yukawa, H.
    Characterization of shikimate dehydrogenase homologues of Corynebacterium glutamicum (2013), Appl. Microbiol. Biotechnol., 97, 8139-8149.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.25 expression in Escherichia coli Corynebacterium glutamicum
1.1.1.282 gene qsuD, recombinant expression in Escherichia coli Corynebacterium glutamicum

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.282 additional information construction of a gene qsuD deletion mutant. Cell cultures of the qsuD-deficient strain in glucose-containing medium are light yellow, whereas those of the wild-type in shikimate or quinate-containing medium are brown Corynebacterium glutamicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.25 additional information not inhibitory: quinate; not inhibitory: quinate Corynebacterium glutamicum
1.1.1.25 shikimate shikimate synthesis decreases with increasing shikimate concentration. The relative activity halves at about 1.4 mM shikimate; shikimate synthesis decreases with increasing shikimate concentration. The relative activity halves at about 1.4 mM shikimate Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.25 0.00036
-
 3-dehydroquinate pH 7.0, 30°C Corynebacterium glutamicum
1.1.1.25 0.14
-
 shikimate pH 9.0, 30°C Corynebacterium glutamicum
1.1.1.25 0.187
-
 shikimate pH 7.0, 30°C Corynebacterium glutamicum
1.1.1.25 0.2
-
 3-dehydroshikimate pH 7.0, 30°C Corynebacterium glutamicum
1.1.1.25 0.272
-
 3-dehydroshikimate pH 9.0, 30°C Corynebacterium glutamicum
1.1.1.25 0.42
-
 3-dehydroquinate pH 7.0, 30°C Corynebacterium glutamicum
1.1.1.282 additional information
-
 additional information Michaelis constant with quinate as substrate is lower than that with shikimate under optimal conditions Corynebacterium glutamicum
1.1.1.282 0.42
-
 3-dehydroquinate pH 7.0, temperature not specified in the publication, recombinant enzyme, with NADH Corynebacterium glutamicum
1.1.1.282 0.444
-
 L-quinate pH 7.0, temperature not specified in the publication, recombinant enzyme, with NAD+ Corynebacterium glutamicum
1.1.1.282 1.141
-
 3-dehydroquinate pH 7.0, temperature not specified in the publication, recombinant enzyme, with NADPH Corynebacterium glutamicum
1.1.1.282 1.299
-
 shikimate pH 7.0, temperature not specified in the publication, recombinant enzyme, with NAD+ Corynebacterium glutamicum
1.1.1.282 5.933
-
 3-dehydroshikimate pH 7.0, temperature not specified in the publication, recombinant enzyme, with NADH Corynebacterium glutamicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.25 30000
-
 x * 30000, SDS-PAGE Corynebacterium glutamicum
1.1.1.282 30000
-
-
 Corynebacterium glutamicum
1.1.1.282 60000
-
 about, gel filtration Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.282 L-quinate + NAD+ Corynebacterium glutamicum
-
 3-dehydroquinate + NADH + H+
-
 ?
1.1.1.282 shikimate + NAD+ Corynebacterium glutamicum
-
 3-dehydroshikimate + NADH + H+
-
 ?
1.1.1.282 shikimate + NAD+ Corynebacterium glutamicum JCM 18229
-
 3-dehydroshikimate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.25 Corynebacterium glutamicum A4QB65
-
-
1.1.1.25 Corynebacterium glutamicum A4QEK4 putative
-
1.1.1.25 Corynebacterium glutamicum JCM 18229 A4QB65
-
-
1.1.1.25 Corynebacterium glutamicum JCM 18229 A4QEK4 putative
-
1.1.1.282 Corynebacterium glutamicum A4QB65
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.282 recombinant enzyme from Escherichia coli Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.25 3-dehydroquinate + NADH + H+
-
 Corynebacterium glutamicum quinate + NAD+
-
 r
1.1.1.25 3-dehydroquinate + NADH + H+
-
 Corynebacterium glutamicum JCM 18229 quinate + NAD+
-
 r
1.1.1.25 3-dehydroquinate + NADPH + H+
-
 Corynebacterium glutamicum quinate + NADP+
-
 r
1.1.1.25 3-dehydroquinate + NADPH + H+
-
 Corynebacterium glutamicum JCM 18229 quinate + NADP+
-
 r
1.1.1.25 3-dehydroshikimate + NADH + H+ less than 1% of the rate with 3-dehydroquinate Corynebacterium glutamicum shikimate + NAD+
-
 r
1.1.1.25 3-dehydroshikimate + NADH + H+ less than 1% of the rate with 3-dehydroquinate Corynebacterium glutamicum JCM 18229 shikimate + NAD+
-
 r
1.1.1.25 3-dehydroshikimate + NADPH + H+
-
 Corynebacterium glutamicum shikimate + NADP+
-
 r
1.1.1.25 3-dehydroshikimate + NADPH + H+
-
 Corynebacterium glutamicum JCM 18229 shikimate + NADP+
-
 r
1.1.1.25 quinate + NAD+
-
 Corynebacterium glutamicum 3-dehydroquinate + NADH + H+
-
 r
1.1.1.25 quinate + NAD+
-
 Corynebacterium glutamicum JCM 18229 3-dehydroquinate + NADH + H+
-
 r
1.1.1.25 shikimate + NADP+
-
 Corynebacterium glutamicum 3-dehydroshikimate + NADPH + H+
-
 r
1.1.1.25 shikimate + NADP+
-
 Corynebacterium glutamicum JCM 18229 3-dehydroshikimate + NADPH + H+
-
 r
1.1.1.282 L-quinate + NAD+
-
 Corynebacterium glutamicum 3-dehydroquinate + NADH + H+
-
 ?
1.1.1.282 additional information QsuD reduces 3-dehydroquinate using NADH and oxidizes quinate using NAD+ as cofactor Corynebacterium glutamicum ?
-
 ?
1.1.1.282 additional information QsuD reduces 3-dehydroquinate using NADH and oxidizes quinate using NAD+ as cofactor Corynebacterium glutamicum JCM 18229 ?
-
 ?
1.1.1.282 shikimate + NAD+
-
 Corynebacterium glutamicum 3-dehydroshikimate + NADH + H+
-
 ?
1.1.1.282 shikimate + NAD+
-
 Corynebacterium glutamicum JCM 18229 3-dehydroshikimate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.25 ? x * 30000, SDS-PAGE Corynebacterium glutamicum
1.1.1.282 homodimer 2 * 30000, about, recombinant enzyme, SDS-PAGE Corynebacterium glutamicum

Synonyms

EC Number Synonyms Comment Organism
1.1.1.25 AroE
-
 Corynebacterium glutamicum
1.1.1.25 cgR_0495
-
 Corynebacterium glutamicum
1.1.1.25 cgR_1677
-
 Corynebacterium glutamicum
1.1.1.25 qsuD
-
 Corynebacterium glutamicum
1.1.1.282 cgR_0495
-
 Corynebacterium glutamicum
1.1.1.282 qsuD
-
 Corynebacterium glutamicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.25 0.36
-
 3-dehydroquinate pH 7.0, 30°C Corynebacterium glutamicum
1.1.1.25 31
-
 shikimate pH 7.0, 30°C Corynebacterium glutamicum
1.1.1.25 114
-
 3-dehydroquinate pH 7.0, 30°C Corynebacterium glutamicum
1.1.1.25 118
-
 3-dehydroshikimate pH 9.0, 30°C Corynebacterium glutamicum
1.1.1.25 234
-
 shikimate pH 9.0, 30°C Corynebacterium glutamicum
1.1.1.25 329
-
 3-dehydroshikimate pH 7.0, 30°C Corynebacterium glutamicum
1.1.1.282 1.75
-
 shikimate pH 7.0, temperature not specified in the publication, recombinant enzyme, with NAD+ Corynebacterium glutamicum
1.1.1.282 5.67
-
 3-dehydroshikimate pH 7.0, temperature not specified in the publication, recombinant enzyme, with NADH Corynebacterium glutamicum
1.1.1.282 9.31
-
 3-dehydroquinate pH 7.0, temperature not specified in the publication, recombinant enzyme, with NADPH Corynebacterium glutamicum
1.1.1.282 14.6
-
 L-quinate pH 7.0, temperature not specified in the publication, recombinant enzyme, with NAD+ Corynebacterium glutamicum
1.1.1.282 114
-
 3-dehydroquinate pH 7.0, temperature not specified in the publication, recombinant enzyme, with NADH Corynebacterium glutamicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.25 6
-
 reduction of 3-dehydroquinate Corynebacterium glutamicum
1.1.1.25 7
-
 reduction of 3-dehydroshikimate Corynebacterium glutamicum
1.1.1.25 8.5
-
 oxidation of quinate Corynebacterium glutamicum
1.1.1.25 9
-
 oxidation of shikimate Corynebacterium glutamicum
1.1.1.282 6
-
 reduction of 3-dehydroquinate with NADH Corynebacterium glutamicum
1.1.1.282 8.5
-
 oxidation of quinate with NAD+ Corynebacterium glutamicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.282 additional information
-
 the catalytic reaction of QsuD is highly susceptible to pH Corynebacterium glutamicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.25 additional information NADH shows about 2% of the activity with NADPH Corynebacterium glutamicum
1.1.1.25 additional information NADPH shows about 2.5% of the activity with NADPH Corynebacterium glutamicum
1.1.1.25 NAD+
-
 Corynebacterium glutamicum
1.1.1.25 NADH
-
 Corynebacterium glutamicum
1.1.1.25 NADP+
-
 Corynebacterium glutamicum
1.1.1.25 NADPH
-
 Corynebacterium glutamicum
1.1.1.282 additional information the turnover number is lower with NADP+ instead of NAD+ as cofactor. NADP(H) is just only for 3-dehydroquinate reduction by the enzyme Corynebacterium glutamicum
1.1.1.282 NAD+
-
 Corynebacterium glutamicum
1.1.1.282 NADH
-
 Corynebacterium glutamicum

Expression

EC Number Organism Comment Expression
1.1.1.25 Corynebacterium glutamicum isoform qsuD mRNA expression in shikimate-grown cells is upregulated relative to that in the glucose-grown cells up
1.1.1.282 Corynebacterium glutamicum qsuD mRNA expression is upregulated in shikimate-grown cells relative to that in the glucose-grown cells up

General Information

EC Number General Information Comment Organism
1.1.1.25 physiological function a strain deficient in isoform qsuD does not grow on either shikimate or quinate as sole carbon sources but grows largely unhindered on glucose Corynebacterium glutamicum
1.1.1.282 physiological function QsuD is essential for growth on shikimate and quinate as sole carbon sources, suggesting that it is the key enzyme for shikimate/quinate utilization Corynebacterium glutamicum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.25 0.143
-
 3-dehydroquinate pH 7.0, 30°C Corynebacterium glutamicum
1.1.1.25 168
-
 shikimate pH 7.0, 30°C Corynebacterium glutamicum
1.1.1.25 272
-
 3-dehydroquinate pH 7.0, 30°C Corynebacterium glutamicum
1.1.1.25 435
-
 3-dehydroshikimate pH 9.0, 30°C Corynebacterium glutamicum
1.1.1.25 1650
-
 3-dehydroshikimate pH 7.0, 30°C Corynebacterium glutamicum
1.1.1.25 1670
-
 shikimate pH 9.0, 30°C Corynebacterium glutamicum
1.1.1.282 0.96
-
 3-dehydroshikimate pH 7.0, temperature not specified in the publication, recombinant enzyme, with NADH Corynebacterium glutamicum
1.1.1.282 1.35
-
 shikimate pH 7.0, temperature not specified in the publication, recombinant enzyme, with NAD+ Corynebacterium glutamicum
1.1.1.282 8.16
-
 3-dehydroquinate pH 7.0, temperature not specified in the publication, recombinant enzyme, with NADPH Corynebacterium glutamicum
1.1.1.282 32.88
-
 L-quinate pH 7.0, temperature not specified in the publication, recombinant enzyme, with NAD+ Corynebacterium glutamicum
1.1.1.282 271.4
-
 3-dehydroquinate pH 7.0, temperature not specified in the publication, recombinant enzyme, with NADH Corynebacterium glutamicum