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Literature summary extracted from

  • Chen, M.M.; Wang, F.Q.; Lin, L.C.; Yao, K.; Wei, D.Z.
    Characterization and application of fusidane antibiotic biosynethsis enzyme 3-ketosteroid-DELTA1-dehydrogenase in steroid transformation (2012), Appl. Microbiol. Biotechnol., 96, 133-142.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.3.99.4 synthesis kstDF is a promising enzyme in steroid DELTA1-dehydrogenation and steroid reduction that is propitious to construct genetically engineered steroid-transforming recombinants by heterologous overexpression Aspergillus fumigatus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.99.4 gene kstDF, DNA and amino acid sequence determination, analysis, and comparison, expression in Escherichia coli strain BL21(lambdaDE3) and in Pichia pastoris strains KM71 and GS115 cytoplasm, both intracellularly and extracelluarly, the latter is inactive, method optimization Aspergillus fumigatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.99.4 cytoplasm
-
 Aspergillus fumigatus 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.99.4 64370
-
 x * 64370, sequence calculation Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.99.4 4-androstene-3,17-dione + FAD Aspergillus fumigatus
-
 1,4-androstadiene-3,17-dione + FADH2
-
 r
1.3.99.4 4-androstene-3,17-dione + FAD Aspergillus fumigatus CICC 40167
-
 1,4-androstadiene-3,17-dione + FADH2
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.4 Aspergillus fumigatus
-
 gene kstDF
-
1.3.99.4 Aspergillus fumigatus CICC 40167
-
 gene kstDF
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.99.4 recombinant enzyme from Pichia pastoris GS115 by hydrophobic interaction chromatography, recombinant His-tagged enzyme from Escherichia coli strain BL21(lambdaDE3) by nickel affinity chromatography Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.99.4 4-androstene-3,17-dione + FAD
-
 Aspergillus fumigatus 1,4-androstadiene-3,17-dione + FADH2
-
 r
1.3.99.4 4-androstene-3,17-dione + FAD
-
 Aspergillus fumigatus CICC 40167 1,4-androstadiene-3,17-dione + FADH2
-
 r

Subunits

EC Number Subunits Comment Organism
1.3.99.4 ? x * 64370, sequence calculation Aspergillus fumigatus

Synonyms

EC Number Synonyms Comment Organism
1.3.99.4 3-ketosteroid-DELTA1-dehydrogenase
-
 Aspergillus fumigatus
1.3.99.4 4-ene-3-oxosteroid:(acceptor)l-ene-oxidoreductase
-
 Aspergillus fumigatus
1.3.99.4 KstDF
-
 Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.99.4 30
-
 assay at Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.99.4 7
-
 assay at Aspergillus fumigatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.99.4 FAD
-
 Aspergillus fumigatus
1.3.99.4 FADH2
-
 Aspergillus fumigatus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.3.99.4 Aspergillus fumigatus sequence calculation
-
 7.81

General Information

EC Number General Information Comment Organism
1.3.99.4 physiological function the enzyme functions in the conversion of 4-ene-3-oxosteroid to 1,4-diene-3-oxosteroid by trans-axial elimination of the Cl and C2 hydrogens, it is active in DELTA1-dehydrogenation in fusidane antibiotic biosynthesis, an important transformations in the synthesis of steroid hormones Aspergillus fumigatus