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Literature summary extracted from

  • Moubasher, H.; Fahmi, A.; Abdur-Rahman, M.
    Induction, purification and molecular characterization of sulfhydryl oxidase from an Egyptian isolates of Aspergillus niger (2012), Appl. Biochem. Microbiol., 48, 290-294.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.3.2 L-Cys fungus is not able to use L-Cys as sulfur source instead of sulfate. Presence of L-Cys induces production of an excessive amount of both intracellular and extracellular enzyme Aspergillus niger
1.8.3.2 MgSO4 in the absence of MgSO4 in the growth medium, growth is weak and no enzyme activity detected. Highest activity at 0.1% MgSO4 in the medium Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.3.2 6.7
-
 glutathione pH 5.5, 25°C Aspergillus niger

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.3.2 extracellular both intra- and extracellular Aspergillus niger
-
-
1.8.3.2 intracellular both intra- and extracellular Aspergillus niger 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.3.2 MgSO4 in the absence of MgSO4 in the growth medium, growth is weak and no enzyme activity detected. Highest activity at 0.1% MgSO4 in the medium Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.3.2 50000
-
 1 * 50000 plus 1 * 55000, SDS-PAGE Aspergillus niger
1.8.3.2 55000
-
 1 * 50000 plus 1 * 55000, SDS-PAGE Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
1.8.3.2 Aspergillus niger
-
-
-
1.8.3.2 Aspergillus niger AUMC 4947
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.3.2
-
 Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.3.2 2 glutathione + O2
-
 Aspergillus niger glutathione disulfide + H2O2
-
 ?
1.8.3.2 2 glutathione + O2
-
 Aspergillus niger AUMC 4947 glutathione disulfide + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.3.2 dimer 1 * 50000 plus 1 * 55000, SDS-PAGE Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.3.2 60
-
-
 Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.3.2 5.5
-
-
 Aspergillus niger