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Literature summary extracted from

  • Karich, A.; Kluge, M.; Ullrich, R.; Hofrichter, M.
    Benzene oxygenation and oxidation by the peroxygenase of Agrocybe aegerita (2013), AMB Express, 3, 5.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.2.1 3.6
-
 Benzene pH 7.0, temperature not specified in the publication Cyclocybe aegerita

Organism

EC Number Organism UniProt Comment Textmining
1.11.2.1 Cyclocybe aegerita B9W4V6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.2.1 benzene + H2O2
-
 Cyclocybe aegerita phenol + H2O oxygenation of the unactivated aromatic ring of benzene with hydrogen peroxide as co-substrate. Reaction proceeds via an initial epoxide intermediate that re-aromatizes in aqueous solution to form phenol. Second and third [per]oxygenation is also observed and results in the formation of further hydroxylation and following [per]oxidation products hydroquinone and p-benzoquinone, catechol and o-benzoquinone as well as 1,2,4-trihydroxybenzene and hydroxy-p-benzoquinone, respectively. The origin of the oxygen atom incorporated into benzene or phenol is the peroxide ?

Synonyms

EC Number Synonyms Comment Organism
1.11.2.1 APO
-
 Cyclocybe aegerita

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.2.1 8
-
 Benzene pH 7.0, temperature not specified in the publication Cyclocybe aegerita

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.2.1 7
-
-
 Cyclocybe aegerita

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.2.1 2.2
-
 Benzene pH 7.0, temperature not specified in the publication Cyclocybe aegerita