Literature summary extracted from

Naumann, P.T.; Lauhon, C.T.; Ficner, R.
Purification, crystallization and preliminary crystallographic analysis of a 4-thiouridine synthetase-RNA complex (2013), Acta Crystallogr. Sect. F, 69, 421-424.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.8.1.B3	expressed in Escherichia coli	Thermotoga maritima
2.8.1.B3	expressed in Escherichia coli BL21(DE3)pLysS cells	Thermotoga maritima

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
2.8.1.B3	in complex with RNA (truncated tRNAPhe), sitting drop vapor diffusion method, using 2 M sodium formate, 100 mM sodium citrate pH 4.6, 2 mM dithiothreitol, at 20°C	Thermotoga maritima
2.8.1.B3	sitting drop vapor diffusion method, using 2.0 M sodium formate, 100 mM sodium citrate pH 4.6, 2 mM dithiothreitol	Thermotoga maritima

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
2.8.1.B3	Mg2+	required	Thermotoga maritima

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.8.1.B3	44000	-	x * 44000	Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2.8.1.B3	sulfurtransferase IscS-SSH + adenylated-tRNA-uridine(position8)	Thermotoga maritima	-	sulfurtransferase IscS-SH + tRNA-4-thiouridine(position8) + AMP	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.8.1.B3	Thermotoga maritima	Q9X220	-	-
2.8.1.B3	Thermotoga maritima	Q9X220	cf. 2.8.1.4	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.8.1.B3	-	Thermotoga maritima
2.8.1.B3	DEAE-Sepharose column chromatography and Superdex 75 gel filtration	Thermotoga maritima

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.8.1.B3	sulfurtransferase IscS-SSH + adenylated-tRNA-uridine(position8)	-	Thermotoga maritima	sulfurtransferase IscS-SH + tRNA-4-thiouridine(position8) + AMP	-	?

Subunits

EC Number	Subunits	Comment	Organism
2.8.1.B3	?	x * 44000, SDS-PAGE	Thermotoga maritima
2.8.1.B3	?	x * 44000	Thermotoga maritima

Synonyms

EC Number	Synonyms	Comment	Organism
2.8.1.B3	4-thiouridine synthetase	-	Thermotoga maritima
2.8.1.B3	ThiI	-	Thermotoga maritima

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Release 2023.1 (January 2023)