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Literature summary extracted from

  • Li, D.F.; Feng, L.; Hou, Y.J.; Liu, W.
    The expression, purification and crystallization of a ubiquitin-conjugating enzyme E2 from Agrocybe aegerita underscore the impactof His-tag location on recombinant protein properties (2013), Acta Crystallogr. Sect. F, 69, 153-157.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.2.23 drug development UbcA1 shows antitumor properties, a potential anticancer drug candidate Cyclocybe aegerita

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.23 subcloned in Escherichia coli BL21(DE2), via pET28 vector, N-terminally His-tagged Cyclocybe aegerita

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.2.23 recombinant enzyme, N-terminally His-tagged, subcloned in Escherichia coli BL21(DE2), via pET28 vector, purified using Ni-NTA column and HiLoad 16/60 Superdex 75 gel filtration column, diffracted X-rays to 1.7 A. The crystal belongs to space group C2 Cyclocybe aegerita

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.23 18000
-
 2 * 18000, single band on SDS-PAGE, indicating a dimeric form. Gel filtration results in an estimated molecular weight of 42 kDa Cyclocybe aegerita
2.3.2.23 42000
-
 gel filtration results in an estimated molecular weight of 42 kDa Cyclocybe aegerita

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.23 Cyclocybe aegerita
-
 medicinal mushroom, basidiomycetes
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.2.23 additional information ubiquitination is involved in myriad cell and disease pathways. The ubiquitin-conjugating E2 is the central player in the ubiquitin-transfer pathway Cyclocybe aegerita

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.23 recombinant enzyme, N-terminally His-tagged, subcloned in Escherichia coli BL21(DE2), via pET28 vector, purified using Ni-NTA column and HiLoad 16/60 Superdex 75 gel filtration column, purity 95% after two steps Cyclocybe aegerita

Subunits

EC Number Subunits Comment Organism
2.3.2.23 dimer 2 * 18000, single band on SDS-PAGE, indicating a dimeric form. Gel filtration results in an estimated molecular weight of 42 kDa Cyclocybe aegerita

Synonyms

EC Number Synonyms Comment Organism
2.3.2.23 UbcA1
-
 Cyclocybe aegerita
2.3.2.23 ubiquitin-conjugating enzyme E2
-
 Cyclocybe aegerita

General Information

EC Number General Information Comment Organism
2.3.2.23 physiological function ubiquitination is involved in myriad cell and disease pathways. The ubiquitin-conjugating E2 is the central player in the ubiquitin-transfer pathway Cyclocybe aegerita