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Literature summary extracted from

  • Vyas, R.; Tewari, R.; Weiss, M.S.; Karthikeyan, S.
    Structures of ternary complexes of aspartate-semialdehyde dehydrogenase (Rv3708c) from Mycobacterium tuberculosis H37Rv (2012), Acta Crystallogr. Sect. D, 68, 671-679.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.11 expressed in Escherichia coli M15 cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.1.11 in complex with S-methyl-l-cysteine sulfoxide and sulfate, sitting drop vapor diffusion method, using 1.6 M ammonium sulfate and 100 mM citric acid pH 5.0 Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.11 L-4-aspartyl phosphate + NADPH + H+ Mycobacterium tuberculosis
-
 L-aspartate 4-semialdehyde + phosphate + NADP+
-
 ?
1.2.1.11 L-4-aspartyl phosphate + NADPH + H+ Mycobacterium tuberculosis H37Rv
-
 L-aspartate 4-semialdehyde + phosphate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.11 Mycobacterium tuberculosis P9WNX5
-
-
1.2.1.11 Mycobacterium tuberculosis H37Rv P9WNX5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.11 Ni-NTA column chromatography and gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.11 L-4-aspartyl phosphate + NADPH + H+
-
 Mycobacterium tuberculosis L-aspartate 4-semialdehyde + phosphate + NADP+
-
 ?
1.2.1.11 L-4-aspartyl phosphate + NADPH + H+
-
 Mycobacterium tuberculosis H37Rv L-aspartate 4-semialdehyde + phosphate + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.11 ASADH
-
 Mycobacterium tuberculosis
1.2.1.11 Asd
-
 Mycobacterium tuberculosis
1.2.1.11 Rv3708c
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.11 NADPH
-
 Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
1.2.1.11 metabolism the enzyme lies at the first branch point in the biosynthetic pathway of important amino acids including lysine and methionine and the cell-wall component diaminopimelate Mycobacterium tuberculosis