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Literature summary extracted from

  • Mihasan, M.; Stefan, M.; Hritcu, L.; Artenie, V.; Brandsch, R.
    Evidence of a plasmid-encoded oxidative xylose-catabolic pathway in Arthrobacter nicotinovorans pAO1 (2013), Res. Microbiol., 164, 22-30.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.179 expressed in Escherichia coli Paenarthrobacter nicotinovorans
1.2.1.4 expressed in Escherichia coli Paenarthrobacter nicotinovorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.179 0.32
-
 NADP+ 28°C, pH 7.8 Paenarthrobacter nicotinovorans
1.1.1.179 5.03
-
 NAD+ 28°C, pH 7.8 Paenarthrobacter nicotinovorans
1.1.1.179 15.2
-
 D-xylose 28°C, pH 7.8, with NADP+ as cofactor Paenarthrobacter nicotinovorans
1.2.1.4 0.0022
-
 NADP+ pH 8.4, 28°C, cosubstrate butyraldehyde Paenarthrobacter nicotinovorans
1.2.1.4 0.11
-
 Glutaraldehyde pH 8.4, 28°C, cosubstrate NADP+ Paenarthrobacter nicotinovorans
1.2.1.4 0.15
-
 Butyraldehyde pH 8.4, 28°C, cosubstrate NADP+ Paenarthrobacter nicotinovorans
1.2.1.4 0.65
-
 glyceraldehyde pH 8.4, 28°C, cosubstrate NADP+ Paenarthrobacter nicotinovorans
1.2.1.4 1.26
-
 NAD+ pH 8.4, 28°C, cosubstrate butyraldehyde Paenarthrobacter nicotinovorans
1.2.1.4 1.5
-
 propionaldehyde pH 8.4, 28°C, cosubstrate NADP+ Paenarthrobacter nicotinovorans
1.2.1.4 2.1
-
 benzaldehyde pH 8.4, 28°C, cosubstrate NADP+ Paenarthrobacter nicotinovorans
1.2.1.4 2.5
-
 acetaldehyde pH 8.4, 28°C, cosubstrate NADP+ Paenarthrobacter nicotinovorans
1.2.1.4 4.2
-
 formaldehyde pH 8.4, 28°C, cosubstrate NADP+ Paenarthrobacter nicotinovorans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.179 Zn2+ 2 mol Zn2+ per monomer Paenarthrobacter nicotinovorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.179 47000
-
 4 * 47000, SDS-PAGE Paenarthrobacter nicotinovorans

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.179 Paenarthrobacter nicotinovorans
-
-
-
1.2.1.4 Paenarthrobacter nicotinovorans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.179 D-xylose + NAD+ very low activity compared to NADP+ Paenarthrobacter nicotinovorans D-xylono-1,5-lactone + NADH + H+
-
 ?
1.1.1.179 D-xylose + NADP+
-
 Paenarthrobacter nicotinovorans D-xylono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.179 additional information at concentrations of up to 50 mM of L-xylose, D-ribose, D-arabinose, L-arabinose, D-glucose, D-galactose or D-fructose, the enzyme produces little NADPH and the specific activity toward these substrates are below 5% of the activity recorded with D-xylose Paenarthrobacter nicotinovorans ?
-
 ?
1.2.1.4 acetaldehyde + NADP+ + H2O
-
 Paenarthrobacter nicotinovorans acetate + NADPH + H+
-
 ?
1.2.1.4 benzaldehyde + NADP+ + H2O
-
 Paenarthrobacter nicotinovorans benzoate + NADPH + H+
-
 ?
1.2.1.4 butyraldehyde + NAD+ + H2O
-
 Paenarthrobacter nicotinovorans butyrate + NADH + H+
-
 ?
1.2.1.4 butyraldehyde + NADP+ + H2O
-
 Paenarthrobacter nicotinovorans butyrate + NADPH + H+
-
 ?
1.2.1.4 formaldehyde + NADP+ + H2O
-
 Paenarthrobacter nicotinovorans formate + NADPH + H+
-
 ?
1.2.1.4 glutaraldehyde + NADP+ + H2O
-
 Paenarthrobacter nicotinovorans glutarate + NADPH + H+
-
 ?
1.2.1.4 glyceraldehyde + NADP+ + H2O
-
 Paenarthrobacter nicotinovorans glycerate + NADPH + H+
-
 ?
1.2.1.4 propionaldehyde + NADP+ + H2O
-
 Paenarthrobacter nicotinovorans propanoate + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.179 homotetramer 4 * 47000, SDS-PAGE Paenarthrobacter nicotinovorans

Synonyms

EC Number Synonyms Comment Organism
1.2.1.4 ALDH
-
 Paenarthrobacter nicotinovorans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.179 1.5
-
 NAD+ 28°C, pH 7.8 Paenarthrobacter nicotinovorans
1.1.1.179 10.5
-
 NADP+ 28°C, pH 7.8 Paenarthrobacter nicotinovorans
1.1.1.179 14.9
-
 D-xylose 28°C, pH 7.8, with NADP+ as cofactor Paenarthrobacter nicotinovorans
1.2.1.4 0.33
-
 formaldehyde pH 8.4, 28°C, cosubstrate NADP+ Paenarthrobacter nicotinovorans
1.2.1.4 0.65
-
 Butyraldehyde pH 8.4, 28°C, cosubstrate NADP+ Paenarthrobacter nicotinovorans
1.2.1.4 0.75
-
 acetaldehyde pH 8.4, 28°C, cosubstrate NADP+ Paenarthrobacter nicotinovorans
1.2.1.4 1.02
-
 NADP+ pH 8.4, 28°C, cosubstrate butyraldehyde Paenarthrobacter nicotinovorans
1.2.1.4 1.2
-
 propionaldehyde pH 8.4, 28°C, cosubstrate NADP+ Paenarthrobacter nicotinovorans
1.2.1.4 1.35
-
 glyceraldehyde pH 8.4, 28°C, cosubstrate NADP+ Paenarthrobacter nicotinovorans
1.2.1.4 3.1
-
 NAD+ pH 8.4, 28°C, cosubstrate butyraldehyde Paenarthrobacter nicotinovorans
1.2.1.4 3.92
-
 Glutaraldehyde pH 8.4, 28°C, cosubstrate NADP+ Paenarthrobacter nicotinovorans
1.2.1.4 4.52
-
 benzaldehyde pH 8.4, 28°C, cosubstrate NADP+ Paenarthrobacter nicotinovorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.179 NAD+ very low activity compared to NADP+ Paenarthrobacter nicotinovorans
1.1.1.179 NADP+ strong preference for NADP+ Paenarthrobacter nicotinovorans
1.2.1.4 NAD+ preference for NADP+ Paenarthrobacter nicotinovorans
1.2.1.4 NADP+ preference for NADP+ Paenarthrobacter nicotinovorans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.179 0.298
-
 NAD+ 28°C, pH 7.8 Paenarthrobacter nicotinovorans
1.1.1.179 0.98
-
 D-xylose 28°C, pH 7.8, with NADP+ as cofactor Paenarthrobacter nicotinovorans
1.1.1.179 32
-
 NADP+ 28°C, pH 7.8 Paenarthrobacter nicotinovorans
1.2.1.4 0.09
-
 formaldehyde pH 8.4, 28°C, cosubstrate NADP+ Paenarthrobacter nicotinovorans
1.2.1.4 0.2
-
 glyceraldehyde pH 8.4, 28°C, cosubstrate NADP+ Paenarthrobacter nicotinovorans
1.2.1.4 0.21
-
 benzaldehyde pH 8.4, 28°C, cosubstrate NADP+ Paenarthrobacter nicotinovorans
1.2.1.4 0.3
-
 acetaldehyde pH 8.4, 28°C, cosubstrate NADP+ Paenarthrobacter nicotinovorans
1.2.1.4 0.35
-
 Glutaraldehyde pH 8.4, 28°C, cosubstrate NADP+ Paenarthrobacter nicotinovorans
1.2.1.4 0.43
-
 Butyraldehyde pH 8.4, 28°C, cosubstrate NADP+ Paenarthrobacter nicotinovorans
1.2.1.4 0.8
-
 propionaldehyde pH 8.4, 28°C, cosubstrate NADP+ Paenarthrobacter nicotinovorans
1.2.1.4 2.4
-
 NAD+ pH 8.4, 28°C, cosubstrate butyraldehyde Paenarthrobacter nicotinovorans
1.2.1.4 46
-
 NADP+ pH 8.4, 28°C, cosubstrate butyraldehyde Paenarthrobacter nicotinovorans