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Literature summary extracted from

  • Rozova, O.N.; Khmelenina, V.N.; Vuilleumier, S.; Trotsenko, Y.A.
    Characterization of recombinant pyrophosphate-dependent 6-phosphofructokinase from halotolerant methanotroph Methylomicrobium alcaliphilum 20Z (2010), Res. Microbiol., 161, 861-868.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.90 expressed in Escherichia coli Rosetta (DE3) cells Methylotuvimicrobium alcaliphilum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.90 additional information not affected by pyruvate, citrate, serine, phosphoglycerate, phosphoenolpyruvate, ATP, ADP, AMP, NADP+, NAD+ and NADPH Methylotuvimicrobium alcaliphilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.90 0.095
-
 D-fructose 1,6-bisphosphate in 50 mM Tris-HCl, pH 7.5, 5 mM MgCl2, at 30°C Methylotuvimicrobium alcaliphilum
2.7.1.90 0.118
-
 diphosphate in 50 mM Tris-HCl, pH 7.5, 5 mM MgCl2, at 30°C Methylotuvimicrobium alcaliphilum
2.7.1.90 0.64
-
 D-fructose 6-phosphate in 50 mM Tris-HCl, pH 7.5, 5 mM MgCl2, at 30°C Methylotuvimicrobium alcaliphilum
2.7.1.90 1.01
-
 D-sedoheptulose 6-phosphate in 50 mM Tris-HCl, pH 7.5, 5 mM MgCl2, at 30°C Methylotuvimicrobium alcaliphilum
2.7.1.90 3.4
-
 phosphate in 50 mM Tris-HCl, pH 7.5, 5 mM MgCl2, at 30°C Methylotuvimicrobium alcaliphilum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.90 Co2+ at low concentration (0.01 mM), Co2+ ions can replace Mg2+ (about 39% of the Mg2+ activity) Methylotuvimicrobium alcaliphilum
2.7.1.90 Mg2+ required for activity Methylotuvimicrobium alcaliphilum
2.7.1.90 Mn2+ at low concentration (0.01 mM), Mn2+ ions can replace Mg2+ (about 39% of the Mg2+ activity) Methylotuvimicrobium alcaliphilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.90 44500
-
 4 * 44500, calculated from amino acid sequence Methylotuvimicrobium alcaliphilum
2.7.1.90 45000
-
 4 * 45000, SDS-PAGE Methylotuvimicrobium alcaliphilum
2.7.1.90 180000
-
 gel filtration Methylotuvimicrobium alcaliphilum

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.90 Methylotuvimicrobium alcaliphilum
-
-
-
2.7.1.90 Methylotuvimicrobium alcaliphilum 20Z
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.90 Ni2-NTA column chromatography Methylotuvimicrobium alcaliphilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.90 diphosphate + D-fructose 6-phosphate the enzyme is highly active, nonallosteric and stringently specific to diphosphate as the phosphoryl donor Methylotuvimicrobium alcaliphilum phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate the enzyme is highly active, nonallosteric and stringently specific to diphosphate as the phosphoryl donor Methylotuvimicrobium alcaliphilum 20Z phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-sedoheptulose 6-phosphate low efficiency Methylotuvimicrobium alcaliphilum phosphate + D-sedoheptulose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-sedoheptulose 6-phosphate low efficiency Methylotuvimicrobium alcaliphilum 20Z phosphate + D-sedoheptulose 1,6-bisphosphate
-
 ?
2.7.1.90 phosphate + D-fructose 1,6-bisphosphate the enzyme is more specific for the reverse reaction substrate D-fructose-1,6-bisphosphate than for the forward reaction substrate Methylotuvimicrobium alcaliphilum diphosphate + D-fructose 6-phosphate
-
 r
2.7.1.90 phosphate + D-fructose 1,6-bisphosphate the enzyme is more specific for the reverse reaction substrate D-fructose-1,6-bisphosphate than for the forward reaction substrate Methylotuvimicrobium alcaliphilum 20Z diphosphate + D-fructose 6-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.1.90 homotetramer 4 * 45000, SDS-PAGE Methylotuvimicrobium alcaliphilum
2.7.1.90 homotetramer 4 * 44500, calculated from amino acid sequence Methylotuvimicrobium alcaliphilum

Synonyms

EC Number Synonyms Comment Organism
2.7.1.90 PPi-PFK
-
 Methylotuvimicrobium alcaliphilum
2.7.1.90 pyrophosphate-dependent 6-phosphofructokinase
-
 Methylotuvimicrobium alcaliphilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.90 30
-
-
 Methylotuvimicrobium alcaliphilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.90 7.5
-
-
 Methylotuvimicrobium alcaliphilum