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Literature summary extracted from

  • Chen, N.; Yang, H.; Niu, J.; Liu, S.
    Determination of kinetic parameters and structure-activity relationships of ginsenosides as inhibitors of cyclin-dependent kinase 5/p25 using ultra-pressure liquid chromatography with triple quadrupole tandem mass spectrometry (2013), Rapid Commun. Mass Spectrom., 27, 985-992.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.22 p25 required for activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.22 ginsenoside F1 noncompetitive inhibition Homo sapiens
2.7.11.22 ginsenoside Rb1 noncompetitive inhibition Homo sapiens
2.7.11.22 ginsenoside Re noncompetitive inhibition Homo sapiens
2.7.11.22 ginsenoside Rf noncompetitive inhibition Homo sapiens
2.7.11.22 ginsenoside Rg1 noncompetitive inhibition Homo sapiens
2.7.11.22 ginsenoside Rg2 noncompetitive inhibition Homo sapiens
2.7.11.22 ginsenoside Rh1 noncompetitive inhibition Homo sapiens
2.7.11.22 additional information ginsenosides as inhibitors of cyclin-dependent kinase 5/p25, structure-activity relationships, overview. Ginsenosides Rd, F2, Rb3, Rb2, Rg3, Rc, and Rh2 are no inhibitors of Cdk5/p25. No inhibition by protopanaxadiol Homo sapiens
2.7.11.22 protopanaxatriol noncompetitive inhibition Homo sapiens
2.7.11.22 roscovitine competitive inhibitor Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.22 0.00285
-
 PKTPKKAKKL pH 7.5, 37°C Homo sapiens
2.7.11.22 0.00522
-
 ATP pH 7.5, 37°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.22 Mg2+ required Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.22 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.22 commercial preparation
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.22 ATP + PKTPKKAKKL
-
 Homo sapiens ADP + PKpTPKKAKKL
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.22 Cdk5
-
 Homo sapiens
2.7.11.22 cyclin-dependent kinase 5
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.22 30 37 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.22 7.5
-
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.22 ATP
-
 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.11.22 0.00025
-
 pH 7.5, 37°C Homo sapiens roscovitine
2.7.11.22 0.00431
-
 pH 7.5, 37°C Homo sapiens protopanaxatriol
2.7.11.22 0.0069
-
 pH 7.5, 37°C Homo sapiens ginsenoside F1
2.7.11.22 0.00728
-
 pH 7.5, 37°C Homo sapiens ginsenoside Rh1
2.7.11.22 0.00785
-
 pH 7.5, 37°C Homo sapiens ginsenoside Rg1
2.7.11.22 0.00901
-
 pH 7.5, 37°C Homo sapiens ginsenoside Re
2.7.11.22 0.00976
-
 pH 7.5, 37°C Homo sapiens ginsenoside Rf
2.7.11.22 0.0108
-
 pH 7.5, 37°C Homo sapiens ginsenoside Rg2
2.7.11.22 0.02496
-
 pH 7.5, 37°C Homo sapiens ginsenoside Rb1

General Information

EC Number General Information Comment Organism
2.7.11.22 additional information effect of ginsenosides on Alzheimer's disease may be involved with the regulation of activities of cdk5/p25. Enzyme activity measurement by mass spectrometry, UPLC/TQMS method development, usage with liquid chromatographic separation of product, overview Homo sapiens