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Literature summary extracted from

  • Ma, K.; Hutchins, A.; Sung, S.J.; Adams, M.W.
    Pyruvate ferredoxin oxidoreductase from the hyperthermophilic archaeon, Pyrococcus furiosus, functions as a CoA-dependent pyruvate decarboxylase (1997), Proc. Natl. Acad. Sci. USA, 94, 9608-9613.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.1 additional information no inhibition by ferredoxin Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.1 additional information
-
 additional information binding affinity of CoA is 0.11 mM Pyrococcus furiosus
4.1.1.1 1.1
-
 pyruvate pH and temperature not specified in the publication Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.1 Pyrococcus furiosus
-
-
-
4.1.1.1 Pyrococcus furiosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.1
-
 Pyrococcus furiosus
4.1.1.1
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.1 pyruvate + CoA + 2 oxidized ferredoxin pyruvate ferredoxin oxidoreductase functions as a CoA-dependent pyruvate decarboxylase. Ferredoxin is not necessary for the pyruvate decarboxylase activity of POR. At 80°C (pH 8.0), the apparent Vm value for pyruvate decarboxylation is about 40% of the apparent Vm value for pyruvate oxidation rate (using Pyrococcus furiosus ferredoxin as the electron acceptor) Pyrococcus furiosus acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+
-
 ?
4.1.1.1 pyruvate pyruvate ferredoxin oxidoreductase functions as a CoA-dependent pyruvate decarboxylase. Ferredoxin is not necessary for the pyruvate decarboxylase activity of POR. At 80°C (pH 8.0), the apparent Vm value for pyruvate decarboxylation is about 40% of the apparent Vm value for pyruvate oxidation rate (using Pyrococcus furiosus ferredoxin as the electron acceptor), 60% at pH 10.2 (80°C) Pyrococcus furiosus acetaldehyde + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.7.1 POR bifunctional enzyme that catalyzes both the oxidative and nonoxidative decarboxylation of pyruvate Pyrococcus furiosus
4.1.1.1 POR bifunctional enzyme that catalyzes both the oxidative and nonoxidative decarboxylation of pyruvate Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.1 90
-
-
 Pyrococcus furiosus
4.1.1.1 90
-
 above Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.1 8
-
-
 Pyrococcus furiosus
4.1.1.1 10
-
-
 Pyrococcus furiosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.7.1 5.5 8.5 pH 5.5: about 60% of maximal activity, pH 8.5: about 35% of maximal activity Pyrococcus furiosus
4.1.1.1 8 11.5 pH 8.0: about 60% of maximal activity, pH 10.0: about 50% of maximal activity Pyrococcus furiosus

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.1 CoA desulfocoenzyme A can substitute for CoA showing that the cofactor plays a structural rather than a catalytic role Pyrococcus furiosus