EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.311 | heterologously expressed as a C-terminal His6-tagged protein in Escherichia coli | Amycolatopsis orientalis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.311 | 0.107 | - |
desvancosaminyl-vancomycin | pH 7.5, 37°C | Amycolatopsis orientalis | |
2.4.1.311 | 0.218 | - |
dTDP-beta-L-4-epi-vancosamine | pH 7.5, 37°C | Amycolatopsis orientalis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.311 | dTDP-beta-L-4-epi-vancosamine + devancosaminyl-vancomycin | Amycolatopsis orientalis | the enzyme is involved in the biosynthesis of the glycopeptide antibiotic chloroeremomycin | dTDP + chloroorienticin B | - |
? | |
2.4.1.311 | dTDP-beta-L-4-epi-vancosamine + devancosaminyl-vancomycin | Amycolatopsis orientalis A82846 | the enzyme is involved in the biosynthesis of the glycopeptide antibiotic chloroeremomycin | dTDP + chloroorienticin B | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.311 | Amycolatopsis orientalis | P96558 | - |
- |
2.4.1.311 | Amycolatopsis orientalis A82846 | P96558 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.311 | - |
Amycolatopsis orientalis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.311 | dTDP-beta-L-4-epi-vancosamine + aglucovancomycin | low activity | Amycolatopsis orientalis | dTDP + chloroorienticin C | - |
? | |
2.4.1.311 | dTDP-beta-L-4-epi-vancosamine + aglucovancomycin | low activity | Amycolatopsis orientalis A82846 | dTDP + chloroorienticin C | - |
? | |
2.4.1.311 | dTDP-beta-L-4-epi-vancosamine + desvancosaminyl-vancomycin | - |
Amycolatopsis orientalis | dTDP + chloroorienticin B | - |
? | |
2.4.1.311 | dTDP-beta-L-4-epi-vancosamine + desvancosaminyl-vancomycin | - |
Amycolatopsis orientalis A82846 | dTDP + chloroorienticin B | - |
? | |
2.4.1.311 | dTDP-beta-L-4-epi-vancosamine + devancosaminyl-vancomycin | the enzyme is involved in the biosynthesis of the glycopeptide antibiotic chloroeremomycin | Amycolatopsis orientalis | dTDP + chloroorienticin B | - |
? | |
2.4.1.311 | dTDP-beta-L-4-epi-vancosamine + devancosaminyl-vancomycin | i.e. desvancosaminyl-vancomycin. Devancoaminyl vancomycin is the preferred acceptor for GtfA. GtfA is unable to use UDP-4-epi-vancosamine as the sugar substrate. GtfA catalyzes the addition of 4-epi-vancosamine to the hydroxyl group of beta-OH-Tyr-6 of devancoaminyl vancomycin. GtfA also transfers 4-epi-vancosamine to the 2-amino analogue of devancoaminyl vancomycin to produce the 2-amino analogue of chloroorienticin B | Amycolatopsis orientalis | dTDP + chloroorienticin B | i.e. 4-epi-balhimicin | ? | |
2.4.1.311 | dTDP-beta-L-4-epi-vancosamine + devancosaminyl-vancomycin | the enzyme is involved in the biosynthesis of the glycopeptide antibiotic chloroeremomycin | Amycolatopsis orientalis A82846 | dTDP + chloroorienticin B | - |
? | |
2.4.1.311 | dTDP-beta-L-4-epi-vancosamine + devancosaminyl-vancomycin | i.e. desvancosaminyl-vancomycin. Devancoaminyl vancomycin is the preferred acceptor for GtfA. GtfA is unable to use UDP-4-epi-vancosamine as the sugar substrate. GtfA catalyzes the addition of 4-epi-vancosamine to the hydroxyl group of beta-OH-Tyr-6 of devancoaminyl vancomycin. GtfA also transfers 4-epi-vancosamine to the 2-amino analogue of devancoaminyl vancomycin to produce the 2-amino analogue of chloroorienticin B | Amycolatopsis orientalis A82846 | dTDP + chloroorienticin B | i.e. 4-epi-balhimicin | ? | |
2.4.1.311 | dTDP-beta-L-4-epi-vancosamine + epivancomycin | low activity | Amycolatopsis orientalis | dTDP + chloroeremomycin | - |
? | |
2.4.1.311 | dTDP-beta-L-4-epi-vancosamine + epivancomycin | low activity | Amycolatopsis orientalis A82846 | dTDP + chloroeremomycin | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.311 | GtfA | - |
Amycolatopsis orientalis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.311 | 37 | - |
assay at | Amycolatopsis orientalis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.311 | additional information | - |
additional information | GtfA transfers 4-epi-vancosamine to vancomycin aglycone or epivancomycin at much reduced rates of less than 0.0008/s | Amycolatopsis orientalis | |
2.4.1.311 | 0.038 | - |
desvancosaminyl-vancomycin | pH 7.5, 37°C | Amycolatopsis orientalis | |
2.4.1.311 | 0.038 | - |
dTDP-beta-L-4-epi-vancosamine | pH 7.5, 37°C | Amycolatopsis orientalis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.311 | 7.5 | - |
- |
Amycolatopsis orientalis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.4.1.311 | physiological function | the enzyme is involved in the biosynthesis of chloroeremomycin | Amycolatopsis orientalis |