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Literature summary extracted from

  • Lu, W.; Oberthür, M.; Leimkuhler, C.; Tao, J.; Kahne, D.; Walsh, C.T.
    Characterization of a regiospecific epivancosaminyl transferase GtfA and enzymatic reconstitution of the antibiotic chloroeremomycin (2004), Proc. Natl. Acad. Sci. USA, 101, 4390-4395.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.311 heterologously expressed as a C-terminal His6-tagged protein in Escherichia coli Amycolatopsis orientalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.311 0.107
-
desvancosaminyl-vancomycin pH 7.5, 37°C Amycolatopsis orientalis
2.4.1.311 0.218
-
dTDP-beta-L-4-epi-vancosamine pH 7.5, 37°C Amycolatopsis orientalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.311 dTDP-beta-L-4-epi-vancosamine + devancosaminyl-vancomycin Amycolatopsis orientalis the enzyme is involved in the biosynthesis of the glycopeptide antibiotic chloroeremomycin dTDP + chloroorienticin B
-
?
2.4.1.311 dTDP-beta-L-4-epi-vancosamine + devancosaminyl-vancomycin Amycolatopsis orientalis A82846 the enzyme is involved in the biosynthesis of the glycopeptide antibiotic chloroeremomycin dTDP + chloroorienticin B
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.311 Amycolatopsis orientalis P96558
-
-
2.4.1.311 Amycolatopsis orientalis A82846 P96558
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.311
-
Amycolatopsis orientalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.311 dTDP-beta-L-4-epi-vancosamine + aglucovancomycin low activity Amycolatopsis orientalis dTDP + chloroorienticin C
-
?
2.4.1.311 dTDP-beta-L-4-epi-vancosamine + aglucovancomycin low activity Amycolatopsis orientalis A82846 dTDP + chloroorienticin C
-
?
2.4.1.311 dTDP-beta-L-4-epi-vancosamine + desvancosaminyl-vancomycin
-
Amycolatopsis orientalis dTDP + chloroorienticin B
-
?
2.4.1.311 dTDP-beta-L-4-epi-vancosamine + desvancosaminyl-vancomycin
-
Amycolatopsis orientalis A82846 dTDP + chloroorienticin B
-
?
2.4.1.311 dTDP-beta-L-4-epi-vancosamine + devancosaminyl-vancomycin the enzyme is involved in the biosynthesis of the glycopeptide antibiotic chloroeremomycin Amycolatopsis orientalis dTDP + chloroorienticin B
-
?
2.4.1.311 dTDP-beta-L-4-epi-vancosamine + devancosaminyl-vancomycin i.e. desvancosaminyl-vancomycin. Devancoaminyl vancomycin is the preferred acceptor for GtfA. GtfA is unable to use UDP-4-epi-vancosamine as the sugar substrate. GtfA catalyzes the addition of 4-epi-vancosamine to the hydroxyl group of beta-OH-Tyr-6 of devancoaminyl vancomycin. GtfA also transfers 4-epi-vancosamine to the 2-amino analogue of devancoaminyl vancomycin to produce the 2-amino analogue of chloroorienticin B Amycolatopsis orientalis dTDP + chloroorienticin B i.e. 4-epi-balhimicin ?
2.4.1.311 dTDP-beta-L-4-epi-vancosamine + devancosaminyl-vancomycin the enzyme is involved in the biosynthesis of the glycopeptide antibiotic chloroeremomycin Amycolatopsis orientalis A82846 dTDP + chloroorienticin B
-
?
2.4.1.311 dTDP-beta-L-4-epi-vancosamine + devancosaminyl-vancomycin i.e. desvancosaminyl-vancomycin. Devancoaminyl vancomycin is the preferred acceptor for GtfA. GtfA is unable to use UDP-4-epi-vancosamine as the sugar substrate. GtfA catalyzes the addition of 4-epi-vancosamine to the hydroxyl group of beta-OH-Tyr-6 of devancoaminyl vancomycin. GtfA also transfers 4-epi-vancosamine to the 2-amino analogue of devancoaminyl vancomycin to produce the 2-amino analogue of chloroorienticin B Amycolatopsis orientalis A82846 dTDP + chloroorienticin B i.e. 4-epi-balhimicin ?
2.4.1.311 dTDP-beta-L-4-epi-vancosamine + epivancomycin low activity Amycolatopsis orientalis dTDP + chloroeremomycin
-
?
2.4.1.311 dTDP-beta-L-4-epi-vancosamine + epivancomycin low activity Amycolatopsis orientalis A82846 dTDP + chloroeremomycin
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.311 GtfA
-
Amycolatopsis orientalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.311 37
-
assay at Amycolatopsis orientalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.311 additional information
-
additional information GtfA transfers 4-epi-vancosamine to vancomycin aglycone or epivancomycin at much reduced rates of less than 0.0008/s Amycolatopsis orientalis
2.4.1.311 0.038
-
desvancosaminyl-vancomycin pH 7.5, 37°C Amycolatopsis orientalis
2.4.1.311 0.038
-
dTDP-beta-L-4-epi-vancosamine pH 7.5, 37°C Amycolatopsis orientalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.311 7.5
-
-
Amycolatopsis orientalis

General Information

EC Number General Information Comment Organism
2.4.1.311 physiological function the enzyme is involved in the biosynthesis of chloroeremomycin Amycolatopsis orientalis