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Literature summary extracted from

  • Wang, D.M.; Yu, H.S.; Song, J.G.; Xu, Y.F.; Jin, F.X.
    Enzyme kinetics of ginsenosidase type IV hydrolyzing 6-O-multi-glycosides of protopanaxatriol type ginsenosides (2012), Proc. Biochem., 47, 133-138.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.194 5.74
-
 ginsenoside Rg2 pH 6, 40°C Aspergillus sp.
3.2.1.194 7.06
-
 ginsenoside R1 pH 6, 40°C Aspergillus sp.
3.2.1.194 7.63
-
 ginsenoside Rg1 pH 6, 40°C Aspergillus sp.
3.2.1.194 9.43
-
 ginsenoside Rf pH 6, 40°C Aspergillus sp.
3.2.1.194 22.2
-
 ginsenoside Re pH 6, 40°C Aspergillus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.194 56000
-
 x * 56000, SDS-PAGE Aspergillus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.194 Aspergillus sp.
-
-
-
3.2.1.194 Aspergillus sp. 39g
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.194
-
 Aspergillus sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.194 a protopanaxatriol-type ginsenoside with two glycosyl residues at position 6 + 2 H2O = a protopanaxatriol-type ginsenoside with no glycosidic modification at position 6 + D-glucopyranose + a monosaccharide overall reaction Aspergillus sp.
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.194 1.7
-
 pH 6, 40°C Aspergillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.194 ginsenoside R1 + H2O the enzyme hydrolyzes the 6-O-L-D-(1->2)-xyloside Aspergillus sp. ginsenoside Rg1 + D-xylose
-
 ?
3.2.1.194 ginsenoside R1 + H2O the enzyme hydrolyzes the 6-O-L-D-(1->2)-xyloside Aspergillus sp. 39g ginsenoside Rg1 + D-xylose
-
 ?
3.2.1.194 ginsenoside Re + H2O the enzyme hydrolyzes the 6-O-alpha-L-(1->2)-rhamnoside Aspergillus sp. ginsenoside Rg1 + L-rhamnose
-
 ?
3.2.1.194 ginsenoside Re + H2O the enzyme hydrolyzes the 6-O-alpha-L-(1->2)-rhamnoside Aspergillus sp. 39g ginsenoside Rg1 + L-rhamnose
-
 ?
3.2.1.194 ginsenoside Rf + H2O the enzyme hydrolyzes 6-O-beta-D-(1->2)-glucoside Aspergillus sp. ginsenoside Rh1 + D-glucose
-
 ?
3.2.1.194 ginsenoside Rf + H2O the enzyme hydrolyzes 6-O-beta-D-(1->2)-glucoside Aspergillus sp. 39g ginsenoside Rh1 + D-glucose
-
 ?
3.2.1.194 ginsenoside Rg1 + H2O the enzyme weakly hydrolyzes 6-O-beta-D-glucoside Aspergillus sp. ginsenoside F1 + D-glucose
-
 ?
3.2.1.194 ginsenoside Rg1 + H2O the enzyme weakly hydrolyzes 6-O-beta-D-glucoside Aspergillus sp. 39g ginsenoside F1 + D-glucose
-
 ?
3.2.1.194 ginsenoside Rg2 + H2O the enzyme hydrolyzes 6-O-alpha-L-(1->2)-rhamnoside Aspergillus sp. ginsenoside Rh1 + L-rhamnose
-
 ?
3.2.1.194 ginsenoside Rh1 + H2O the enzyme weakly hydrolyzes 6-O-beta-D-glucoside Aspergillus sp. protopanaxatriol + D-glucose
-
 ?
3.2.1.194 additional information the ginsenosidase type IV can hydrolyze 6-O-multiglycosides including rhamnoside, xyloside and glucoside of protopanaxatriol (PPT) type ginsenosides, but cannot hydrolyze the glycosides of protopanaxadiol (PPD) type ginsenoside Aspergillus sp. ?
-
 ?
3.2.1.194 additional information the ginsenosidase type IV can hydrolyze 6-O-multiglycosides including rhamnoside, xyloside and glucoside of protopanaxatriol (PPT) type ginsenosides, but cannot hydrolyze the glycosides of protopanaxadiol (PPD) type ginsenoside Aspergillus sp. 39g ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.194 ? x * 56000, SDS-PAGE Aspergillus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.194 40
-
 assay at Aspergillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.194 6
-
 assay at Aspergillus sp.