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Literature summary extracted from

  • Trobacher, C.; Clark, S.; Bozzo, G.; Mullen, R.; DeEll, J.; Shelp, B.
    Catabolism of GABA in apple fruit: Subcellular localization and biochemical characterization of two gamma-aminobutyrate transaminases (2013), Postharvest Biol. Technol., 75, 106-113.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.19 removal of the N-terminal targeting presequences yields good recovery of the soluble recombinant proteins in Escherichia coli when they are co-expressed with the GroES/EL molecular chaperone complex Malus domestica
2.6.1.96 removal of the N-terminal targeting presequences yields good recovery of the soluble recombinant proteins in Escherichia coli when they are co-expressed with the GroES/EL molecular chaperone complex Malus domestica

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.19 5-amino-1,3-cyclohexadienylcarboxylate
-
 Malus domestica
2.6.1.19 Aminooxyacetate
-
 Malus domestica
2.6.1.19 beta-Alanine moderate inhibitor; moderate inhibitor Malus domestica
2.6.1.19 ornithine moderate inhibitor; moderate inhibitor Malus domestica
2.6.1.96 Aminooxyacetate effective inhibition at 250 microM Malus domestica
2.6.1.96 beta-Alanine 43–65% inhibition at 1 mM Malus domestica
2.6.1.96 gabaculine i.e. 5-amino-1,3-cyclohexadienylcarboxylate, effective inhibition at 250 microM Malus domestica
2.6.1.96 ornithine 43–65% inhibition at 1 mM Malus domestica
2.6.1.96 vigabatrin 93% inhibition at 1 mM Malus domestica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.19 0.0165
-
 glyoxylate pH 9, 25°C, fixed substrate: 4-aminobutanoate Malus domestica
2.6.1.19 0.0188
-
 pyruvate pH 9, 25°C, fixed substrate: 4-aminobutanoate Malus domestica
2.6.1.19 0.023
-
 pyruvate pH 9, 25°C, fixed substrate: 4-aminobutanoate Malus domestica
2.6.1.19 0.039
-
 glyoxylate pH 9, 25°C, fixed substrate: 4-aminobutanoate Malus domestica
2.6.1.19 0.077
-
 4-aminobutanoate pH 9, 25°C, fixed substrate: pyruvate Malus domestica
2.6.1.19 0.099
-
 4-aminobutanoate pH 9, 25°C, fixed substrate: glyoxylate Malus domestica
2.6.1.19 0.135
-
 4-aminobutanoate pH 9, 25°C, fixed substrate: glyoxylate Malus domestica
2.6.1.19 0.165
-
 4-aminobutanoate pH 9, 25°C, fixed substrate: pyruvate Malus domestica
2.6.1.96 0.017
-
 glyoxylate pH 9.0, 25°C Malus domestica
2.6.1.96 0.019
-
 pyruvate pH 9.0, 25°C Malus domestica
2.6.1.96 0.077
-
 4-aminobutanoate cosubstrate pyruvate, pH 9.0, 25°C Malus domestica
2.6.1.96 0.1
-
 4-aminobutanoate cosubstrate glyoxylate, pH 9.0, 25°C Malus domestica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.19 mitochondrion
-
 Malus domestica 5739
-
2.6.1.96 mitochondrion
-
 Malus domestica 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.19 54200
-
 SDS-PAGE, single recombinant truncated MdGABA-T Malus domestica

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.19 Malus domestica J9XGP8
-
-
2.6.1.19 Malus domestica J9XGZ5
-
-
2.6.1.96 Malus domestica J9XGZ5 Borkh. cv. Empire
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.19 4-aminobutanoate + glyoxylate
-
 Malus domestica 4-oxobutanoate + glycine
-
 ?
2.6.1.19 4-aminobutanoate + pyruvate
-
 Malus domestica 4-oxobutanoate + L-alanine
-
 ?
2.6.1.96 4-aminobutanoate + glyoxylate
-
 Malus domestica succinate semialdehyde + glycine
-
 ?
2.6.1.96 4-aminobutanoate + pyruvate
-
 Malus domestica succinate semialdehyde + L-alanine
-
 ?
2.6.1.96 additional information enzyme utilizes pyruvate and glyoxylate, but not 2-oxoglutarate Malus domestica ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.19 GABA-T
-
 Malus domestica
2.6.1.19 gamma-aminobutyrate transaminase
-
 Malus domestica
2.6.1.19 MdGABA-T1
-
 Malus domestica
2.6.1.19 MdGABA-T2
-
 Malus domestica
2.6.1.96 GABA-T2
-
 Malus domestica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.19 25
-
 assay at Malus domestica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.19 0.74
-
 glyoxylate pH 9, 25°C, fixed substrate: 4-aminobutanoate Malus domestica
2.6.1.19 0.8
-
 pyruvate pH 9, 25°C, fixed substrate: 4-aminobutanoate Malus domestica
2.6.1.19 0.84
-
 4-aminobutanoate pH 9, 25°C, fixed substrate: pyruvate Malus domestica
2.6.1.19 1
-
 4-aminobutanoate pH 9, 25°C, fixed substrate: glyoxylate Malus domestica
2.6.1.19 2.6
-
 glyoxylate pH 9, 25°C, fixed substrate: 4-aminobutanoate Malus domestica
2.6.1.19 2.6
-
 4-aminobutanoate pH 9, 25°C, fixed substrate: pyruvate Malus domestica
2.6.1.19 2.7
-
 pyruvate pH 9, 25°C, fixed substrate: 4-aminobutanoate Malus domestica
2.6.1.19 2.7
-
 4-aminobutanoate pH 9, 25°C, fixed substrate: glyoxylate Malus domestica
2.6.1.96 0.74
-
 glyoxylate pH 9.0, 25°C Malus domestica
2.6.1.96 0.8
-
 pyruvate pH 9.0, 25°C Malus domestica
2.6.1.96 0.84
-
 4-aminobutanoate cosubstrate pyruvate, pH 9.0, 25°C Malus domestica
2.6.1.96 1
-
 4-aminobutanoate cosubstrate glyoxylate, pH 9.0, 25°C Malus domestica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.19 9
-
 assay at Malus domestica
2.6.1.96 8.5 9.5
-
 Malus domestica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.19 8.5 9.5 maximal activity Malus domestica

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.19 pyridoxal 5'-phosphate
-
 Malus domestica

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.6.1.19 10.4
-
 4-aminobutanoate pH 9, 25°C, fixed substrate: glyoxylate Malus domestica
2.6.1.19 10.9
-
 4-aminobutanoate pH 9, 25°C, fixed substrate: pyruvate Malus domestica
2.6.1.19 16.1
-
 4-aminobutanoate pH 9, 25°C, fixed substrate: pyruvate Malus domestica
2.6.1.19 19.5
-
 4-aminobutanoate pH 9, 25°C, fixed substrate: glyoxylate Malus domestica
2.6.1.19 42.7
-
 pyruvate pH 9, 25°C, fixed substrate: 4-aminobutanoate Malus domestica
2.6.1.19 45.7
-
 glyoxylate pH 9, 25°C, fixed substrate: 4-aminobutanoate Malus domestica
2.6.1.19 66.6
-
 glyoxylate pH 9, 25°C, fixed substrate: 4-aminobutanoate Malus domestica
2.6.1.19 116
-
 pyruvate pH 9, 25°C, fixed substrate: 4-aminobutanoate Malus domestica
2.6.1.96 10.4
-
 4-aminobutanoate cosubstrate glyoxylate, pH 9.0, 25°C Malus domestica
2.6.1.96 10.9
-
 4-aminobutanoate cosubstrate pyruvate, pH 9.0, 25°C Malus domestica
2.6.1.96 42.7
-
 pyruvate pH 9.0, 25°C Malus domestica
2.6.1.96 45.6
-
 glyoxylate pH 9.0, 25°C Malus domestica