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Literature summary extracted from

  • Nair, D.R.; Ghosh, R.; Manocha, A.; Mohanty, D.; Saran, S.; Gokhale, R.S.
    Two functionally distinctive phosphopantetheinyl transferases from amoeba Dictyostelium discoideum (2011), PLoS ONE, 6, e24262.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.7 expressed in Escherichia coli BL21(DE3) cells Dictyostelium discoideum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.7 mitochondrion
-
 Dictyostelium discoideum 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.8.7 20000
-
 x * 20000, isoform AcpS, calculated from amino acid sequence Dictyostelium discoideum
2.7.8.7 27000
-
 x * 27000, isoform AcpS, SDS-PAGE Dictyostelium discoideum
2.7.8.7 34000
-
 x * 34000, isoform Sfp, calculated from amino acid sequence Dictyostelium discoideum
2.7.8.7 34000
-
 x * 34000, isoform Sfp, SDS-PAGE Dictyostelium discoideum

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.7 Dictyostelium discoideum Q54MI2 isoform Sfp
-
2.7.8.7 Dictyostelium discoideum Q552R4 isoform AcpS
-
2.7.8.7 Dictyostelium discoideum AX2 Q54MI2 isoform Sfp
-
2.7.8.7 Dictyostelium discoideum AX2 Q552R4 isoform AcpS
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.7 Ni-NTA column chromatography Dictyostelium discoideum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.7 acetyl-CoA + acyl-carrier protein substrate for isoform AcpS Dictyostelium discoideum CoA + acetyl-[acyl-carrier protein]
-
 ?
2.7.8.7 acetyl-CoA + acyl-carrier protein substrate for isoform AcpS Dictyostelium discoideum AX2 CoA + acetyl-[acyl-carrier protein]
-
 ?
2.7.8.7 acetyl-CoA + polyketide synthase 1 substrate for isoform Sfp Dictyostelium discoideum CoA + acetyl-polyketide synthase 1
-
 ?
2.7.8.7 acetyl-CoA + polyketide synthase 1 substrate for isoform Sfp Dictyostelium discoideum AX2 CoA + acetyl-polyketide synthase 1
-
 ?
2.7.8.7 acetyl-CoA + polyketide synthase 16 substrate for isoform Sfp Dictyostelium discoideum CoA + acetyl-polyketide synthase 16
-
 ?
2.7.8.7 acetyl-CoA + polyketide synthase 16 substrate for isoform Sfp Dictyostelium discoideum AX2 CoA + acetyl-polyketide synthase 16
-
 ?
2.7.8.7 acetyl-CoA + polyketide synthase 2 substrate for isoform Sfp Dictyostelium discoideum CoA + acetyl-polyketide synthase 2
-
 ?
2.7.8.7 acetyl-CoA + polyketide synthase 2 substrate for isoform Sfp Dictyostelium discoideum AX2 CoA + acetyl-polyketide synthase 2
-
 ?
2.7.8.7 additional information isoform AcpS shows weak activity with mycobacterial polyketide synthase 2 Dictyostelium discoideum ?
-
 ?
2.7.8.7 additional information isoform Sfp exhibits weak activity with acetyl-CoA Dictyostelium discoideum ?
-
 ?
2.7.8.7 additional information isoform Sfp exhibits weak activity with acetyl-CoA Dictyostelium discoideum AX2 ?
-
 ?
2.7.8.7 additional information isoform AcpS shows weak activity with mycobacterial polyketide synthase 2 Dictyostelium discoideum AX2 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.8.7 ? x * 20000, isoform AcpS, calculated from amino acid sequence Dictyostelium discoideum
2.7.8.7 ? x * 27000, isoform AcpS, SDS-PAGE Dictyostelium discoideum
2.7.8.7 ? x * 34000, isoform Sfp, calculated from amino acid sequence Dictyostelium discoideum
2.7.8.7 ? x * 34000, isoform Sfp, SDS-PAGE Dictyostelium discoideum

Synonyms

EC Number Synonyms Comment Organism
2.7.8.7 AcpS isoform Dictyostelium discoideum
2.7.8.7 phosphopantetheinyl transferase
-
 Dictyostelium discoideum
2.7.8.7 PPTase
-
 Dictyostelium discoideum
2.7.8.7 Sfp isoform Dictyostelium discoideum

General Information

EC Number General Information Comment Organism
2.7.8.7 metabolism isoform AcpS specifically modifies a stand-alone acyl carrier protein that possesses a mitochondrial import signal Dictyostelium discoideum
2.7.8.7 metabolism isoform Sfp in contrast is specific to type I multifunctional polyketide synthase/fatty acid synthase proteins and cannot modify the stand-alone acyl carrier protein Dictyostelium discoideum