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Literature summary extracted from

  • Kim, Y.S.; Cho, J.H.; Park, S.; Han, J.Y.; Back, K.; Choi, Y.E.
    Gene regulation patterns in triterpene biosynthetic pathway driven by overexpression of squalene synthase and methyl jasmonate elicitation in Bupleurum falcatum (2011), Planta, 233, 343-355.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.21 gene BfSS1, overexpression in Bupleurum falcatum using transfection via Agrobacterium tumefaciens strain GV3101 Bupleurum falcatum

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.21 additional information expression in sense and antisense orientation, phenotypes, overview Bupleurum falcatum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.21 47000
-
 x * 47000, about, sequence calculation Bupleurum falcatum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.17 squalene + AH2 + O2 Bupleurum falcatum
-
 (S)-squalene-2,3-epoxide + A + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.17 Bupleurum falcatum
-
-
-
2.5.1.21 Bupleurum falcatum Q32R64 gene BfSS1
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.17 root
-
 Bupleurum falcatum
-
2.5.1.21 root
-
 Bupleurum falcatum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.17 squalene + AH2 + O2
-
 Bupleurum falcatum (S)-squalene-2,3-epoxide + A + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.21 ? x * 47000, about, sequence calculation Bupleurum falcatum

Synonyms

EC Number Synonyms Comment Organism
1.14.14.17 squalene epoxidase
-
 Bupleurum falcatum

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.17 NADPH
-
 Bupleurum falcatum

Expression

EC Number Organism Comment Expression
1.14.14.17 Bupleurum falcatum overexpression of the squalene synthase 1 gene in the sense orientation increases the mRNA accumulation of downstream genes such as squalene epoxidase. Methyl jasmonate treatment of wild type roots enhances the expression of the enzyme up
2.5.1.21 Bupleurum falcatum BfSS1 mRNA accumulates ubiquitously in plant organs and markedly increases in roots after treatment with methyl jasmonate, abscisic acid, and ethephon up

General Information

EC Number General Information Comment Organism
2.5.1.21 metabolism squalene synthase catalyzes the first enzymatic step of the central isoprenoid pathway in sterol and triterpenoid biosynthesis Bupleurum falcatum
2.5.1.21 additional information methyl jasmonate, abscisic acid, and ethephon induce the accumulation of BfSS1 mRNA, overexpression of the BfSS1 gene in the sense orientation in Bupleurum falcatum increases the mRNA accumulation of downstream genes such as squalene epoxidase and cycloartenol synthase but decreases the mRNA levels of beta-amyrin synthase, a triterpene synthase mRNA. Methyljasmonate treatment of transgenic roots overexpressing BfSS1 in the sense orientation fails to stimulate beta-amyrin synthase mRNA accumulation but still enhances saikosaponin and phytosterol production Bupleurum falcatum
2.5.1.21 physiological function squalene synthase functions as a key regulator in channeling the carbon flux into both the primary and secondary metabolite branches, and squalene synthase may play a regulatory role in directing triterpene intermediates and sterol pathways Bupleurum falcatum