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Literature summary extracted from

  • Gus-Mayer, S.; Brunner, H.; Schneider-Poetsch, H.A.
    Rüdiger, W.: Avenacosidase from oat: purification, sequence analysis and biochemical characterization of a new member of the BGA family of beta-glucosidases (1994), Plant Mol. Biol., 26, 909-921.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.186 the enzyme is highly aggregated. It consists of 300-350 kDa aggregates and multimers thereof. Dissociation by freezing and thawing leads to complete loss of enzyme activity Avena sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.186 plastid
-
 Avena sativa 9536
-
3.2.1.188 etioplast
-
 Avena sativa 9513
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.186 60000
-
 x * 60000, the enzyme is highly aggregated. It consists of 300-350 kDa aggregates and multimers thereof Avena sativa
3.2.1.188 60000
-
 5 or 6 * 60000, SDS-PAGE Avena sativa
3.2.1.188 300000 350000 gel filtration Avena sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.186 avenacoside A + H2O Avena sativa the enzyme belongs to a preformed defence system of oat against fungal infection 26-deglucoavenacoside A + D-glucose
-
 ?
3.2.1.186 avenacoside B + H2O Avena sativa the enzyme belongs to a preformed defence system of oat against fungal infection 26-deglucoavenacoside B + D-glucose
-
 ?
3.2.1.188 avenacoside A + H2O Avena sativa
-
 26-desgluco-avenacoside A + D-glucose
-
 ?
3.2.1.188 avenacoside B + H2O Avena sativa
-
 26-desgluco-avenacoside B + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.186 Avena sativa Q38786
-
-
3.2.1.188 Avena sativa
-
 cultivar Pirol
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.186
-
 Avena sativa
3.2.1.188 ammonium sulfate precipitation, hydroxyapatite column chromatography, and Biogel A1.5 gel filtration Avena sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.186 seedling etiolated Avena sativa
-
3.2.1.188 caryopsis
-
 Avena sativa
-
3.2.1.188 coleoptile
-
 Avena sativa
-
3.2.1.188 leaf primary leaves Avena sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.188 2.72
-
 purified enzyme, using 4-nitrophenyl-beta-D-glucoside as substrate at pH 5.4 and 34°C Avena sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.186 avenacoside A + H2O
-
 Avena sativa 26-deglucoavenacoside A + D-glucose
-
 ?
3.2.1.186 avenacoside A + H2O the enzyme belongs to a preformed defence system of oat against fungal infection Avena sativa 26-deglucoavenacoside A + D-glucose
-
 ?
3.2.1.186 avenacoside B + H2O
-
 Avena sativa 26-deglucoavenacoside B + D-glucose
-
 ?
3.2.1.186 avenacoside B + H2O the enzyme belongs to a preformed defence system of oat against fungal infection Avena sativa 26-deglucoavenacoside B + D-glucose
-
 ?
3.2.1.186 additional information 4-nitrophenyl derivatives serve as substrates, in decreasing order: beta-fucoside, beta-glucoside, beta-galactoside, beta-xyloside. The corresponding 2-nitrophenyl glycosides are less well accepted. No hydrolysis is found with alpha-glycosides and beta-thioglucoside Avena sativa ?
-
 ?
3.2.1.188 3-nitrophenyl beta-D-galactopyranoside + H2O
-
 Avena sativa 3-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.188 3-nitrophenyl beta-D-glucopyranoside + H2O
-
 Avena sativa 3-nitrophenol + D-glucopyranose
-
 ?
3.2.1.188 4-nitrophenyl beta-D-fucoside + H2O highest activity Avena sativa 4-nitrophenol + D-fucose
-
 ?
3.2.1.188 4-nitrophenyl beta-D-galactoside + H2O
-
 Avena sativa 4-nitrophenol + D-galactose
-
 ?
3.2.1.188 4-nitrophenyl beta-D-glucoside + H2O
-
 Avena sativa 4-nitrophenol + D-glucose
-
 ?
3.2.1.188 4-nitrophenyl beta-D-xylopyranoside + H2O lowest activity Avena sativa 4-nitrophenol + D-xylose
-
 ?
3.2.1.188 avenacoside A + H2O
-
 Avena sativa 26-desgluco-avenacoside A + D-glucose
-
 ?
3.2.1.188 avenacoside B + H2O
-
 Avena sativa 26-desgluco-avenacoside B + D-glucose
-
 ?
3.2.1.188 additional information no activity with alpha-glycosides, beta-thioglucoside,cellobiose (beta1-4), gentiobiose (beta 1-6), laminaribiose (beta1-3), alpha-sophorose (beta1-2), 4-nitrophenyl-beta-D-xyloside, 3-nitrophenyl-beta-D-cellobioside, 4-nitrophenyl-alpha-D-glucoside, 4-nitrophenyl-alpha-D-galactoside, and 4-nitrophenyl-1-thio-beta-D-glucoside Avena sativa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.186 multimer x * 60000, the enzyme is highly aggregated. It consists of 300-350 kDa aggregates and multimers thereof Avena sativa
3.2.1.188 pentamer or hexamer 5 or 6 * 60000, SDS-PAGE Avena sativa

Synonyms

EC Number Synonyms Comment Organism
3.2.1.186 avenacosidase
-
 Avena sativa
3.2.1.188 26-desgluco-avenacosidase
-
 Avena sativa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.188
-
-
 dissociation by freezing/thawing leads to complete loss of enzyme activity Avena sativa