BRENDA - Enzyme Database

Two glycosyltransferases involved in anthocyanin modification delineated by transcriptome independent component analysis in Arabidopsis thaliana

Yonekura-Sakakibara, K.; Fukushima, A.; Nakabayashi, R.; Hanada, K.; Matsuda, F.; Sugawara, S.; Inoue, E.; Kuromori, T.; Ito, T.; Shinozaki, K.; Wangwattana, B.; Yamazaki, M.; Saito, K.; Plant J. 69, 154-167 (2012)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
2.4.2.51
gene ugt79b1, DNA and amino acid sequence determination and analysis, expression analysis and phylogenetic analysis, recombinant expression as His-tagged protein in Escherichia coli
Arabidopsis thaliana
2.4.2.51
recombinantly expressed as a His-tagged fusion protein
Arabidopsis thaliana
Engineering
EC Number
Protein Variants
Commentary
Organism
2.4.2.51
additional information
construction of transposon insertion mutants of UGT79B1
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.4.2.51
UDP-alpha-D-xylose + cyanidin 3-O-beta-D-glucoside
Arabidopsis thaliana
-
UDP + cyanidin 3-O-beta-D-sambubioside
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.4.2.51
Arabidopsis thaliana
Q9LVW3
-
-
2.4.2.51
Arabidopsis thaliana
Q9LVW3
gene ugt79b1
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.4.2.51
recombinant His-tagged enzyme from Escherichia coli
Arabidopsis thaliana
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.4.2.51
additional information
enzyme converts cyanidin 3-O-glucoside to cyanidin 3-O-xylosyl(1-2)glucoside. Enzyme recognizes 3-O-glucosylated anthocyanidins/flavonols and uridine diphosphate (UDP)-xylose
723428
Arabidopsis thaliana
?
-
-
-
?
2.4.2.51
additional information
UGT79B1 recognizes 3-O-glucosylated anthocyanidins/flavonols and UDP-xylose, but not 3,5-O-diglucosylated anthocyanidins, substrate specificity, overview. No activity with cyanidin 3-O-rhamnoside, cyanidin 3,5-O-diglucoside, cyanidin 3-O-6''-O-4-coumaroylglucoside-5-O-glucoside, cyanidin 3-O-6''-O-p-coumaroylglucoside, kaempferol 3-O-rhanoside, and kaempferol 3-O-glucoside-7-O-rhamnoside. The enzyme is absolutely specific for UDP-xylose as donar substrate, no activity with UDP-arabinose, UDP-galactose, UDP-glucose, UDP-glucuronic acid, and UDP-rhamnose
723428
Arabidopsis thaliana
?
-
-
-
?
2.4.2.51
UDP-alpha-D-xylose + cyanidin 3-O-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + cyanidin 3-O-beta-D-xylosyl-(1->2)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside
-
-
-
?
2.4.2.51
UDP-alpha-D-xylose + cyanidin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + cyanidin 3-O-beta-D-sambubioside
-
-
-
?
2.4.2.51
UDP-alpha-D-xylose + delphinidin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + delphinidin 3-O-beta-D-sambubioside
-
-
-
?
2.4.2.51
UDP-alpha-D-xylose + kaempferol 3-O-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + kaempferol 3-O-beta-D-xylosyl-(1->2)-[alpha-L-rhamnosyl-(1->6)]-beta-D-glucoside
-
-
-
?
2.4.2.51
UDP-alpha-D-xylose + kaempferol 3-O-beta-D-glucoside
best substrate
723428
Arabidopsis thaliana
UDP + kaempferol 3-O-beta-D-xylosyl-(1->2)-beta-D-glucoside
-
-
-
?
2.4.2.51
UDP-alpha-D-xylose + pelargonidin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + pelargonidin 3-O-beta-D-sambubioside
-
-
-
?
2.4.2.51
UDP-alpha-D-xylose + quercetin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + quercetin 3-O-beta-D-sambubioside
-
-
-
?
2.4.2.51
UDP-D-xylose + cyanidin 3-O-(alpha-L-rhamnosyl-(1->6)-beta-D-glucoside)
-
723428
Arabidopsis thaliana
UDP + cyanidin 3-O-(beta-D-xylosyl-1(->2)[alpha-L-rhamnosyl-(1->6)]-beta-D-glucoside)
-
-
-
?
2.4.2.51
UDP-D-xylose + cyanidin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + cyanidin 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside)
-
-
-
?
2.4.2.51
UDP-D-xylose + delphinidin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + delphinidin 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside)
-
-
-
?
2.4.2.51
UDP-D-xylose + kaempferol 3-O-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + kaempferol 3-O-(beta-D-xylosyl-(1->2)[alpha-L-rhamnosyl-(1->6)]-beta-D-glucoside)
-
-
-
?
2.4.2.51
UDP-D-xylose + kaempferol 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + kaempferol 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside)
-
-
-
?
2.4.2.51
UDP-D-xylose + pelargonidin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + pelargonidin 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside)
-
-
-
?
2.4.2.51
UDP-D-xylose + quercetin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + quercetin 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside)
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
2.4.2.51
anthocyanin 3-O-glucoside: 2''-O-xylosyltransferase
-
Arabidopsis thaliana
2.4.2.51
UGT79B1
-
Arabidopsis thaliana
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.51
30
-
assay at
Arabidopsis thaliana
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.51
6.5
-
-
Arabidopsis thaliana
2.4.2.51
7.5
-
assay at
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.2.51
gene ugt79b1, DNA and amino acid sequence determination and analysis, expression analysis and phylogenetic analysis, recombinant expression as His-tagged protein in Escherichia coli
Arabidopsis thaliana
2.4.2.51
recombinantly expressed as a His-tagged fusion protein
Arabidopsis thaliana
Engineering (protein specific)
EC Number
Protein Variants
Commentary
Organism
2.4.2.51
additional information
construction of transposon insertion mutants of UGT79B1
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.4.2.51
UDP-alpha-D-xylose + cyanidin 3-O-beta-D-glucoside
Arabidopsis thaliana
-
UDP + cyanidin 3-O-beta-D-sambubioside
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.2.51
recombinant His-tagged enzyme from Escherichia coli
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.4.2.51
additional information
enzyme converts cyanidin 3-O-glucoside to cyanidin 3-O-xylosyl(1-2)glucoside. Enzyme recognizes 3-O-glucosylated anthocyanidins/flavonols and uridine diphosphate (UDP)-xylose
723428
Arabidopsis thaliana
?
-
-
-
?
2.4.2.51
additional information
UGT79B1 recognizes 3-O-glucosylated anthocyanidins/flavonols and UDP-xylose, but not 3,5-O-diglucosylated anthocyanidins, substrate specificity, overview. No activity with cyanidin 3-O-rhamnoside, cyanidin 3,5-O-diglucoside, cyanidin 3-O-6''-O-4-coumaroylglucoside-5-O-glucoside, cyanidin 3-O-6''-O-p-coumaroylglucoside, kaempferol 3-O-rhanoside, and kaempferol 3-O-glucoside-7-O-rhamnoside. The enzyme is absolutely specific for UDP-xylose as donar substrate, no activity with UDP-arabinose, UDP-galactose, UDP-glucose, UDP-glucuronic acid, and UDP-rhamnose
723428
Arabidopsis thaliana
?
-
-
-
?
2.4.2.51
UDP-alpha-D-xylose + cyanidin 3-O-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + cyanidin 3-O-beta-D-xylosyl-(1->2)-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside
-
-
-
?
2.4.2.51
UDP-alpha-D-xylose + cyanidin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + cyanidin 3-O-beta-D-sambubioside
-
-
-
?
2.4.2.51
UDP-alpha-D-xylose + delphinidin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + delphinidin 3-O-beta-D-sambubioside
-
-
-
?
2.4.2.51
UDP-alpha-D-xylose + kaempferol 3-O-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + kaempferol 3-O-beta-D-xylosyl-(1->2)-[alpha-L-rhamnosyl-(1->6)]-beta-D-glucoside
-
-
-
?
2.4.2.51
UDP-alpha-D-xylose + kaempferol 3-O-beta-D-glucoside
best substrate
723428
Arabidopsis thaliana
UDP + kaempferol 3-O-beta-D-xylosyl-(1->2)-beta-D-glucoside
-
-
-
?
2.4.2.51
UDP-alpha-D-xylose + pelargonidin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + pelargonidin 3-O-beta-D-sambubioside
-
-
-
?
2.4.2.51
UDP-alpha-D-xylose + quercetin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + quercetin 3-O-beta-D-sambubioside
-
-
-
?
2.4.2.51
UDP-D-xylose + cyanidin 3-O-(alpha-L-rhamnosyl-(1->6)-beta-D-glucoside)
-
723428
Arabidopsis thaliana
UDP + cyanidin 3-O-(beta-D-xylosyl-1(->2)[alpha-L-rhamnosyl-(1->6)]-beta-D-glucoside)
-
-
-
?
2.4.2.51
UDP-D-xylose + cyanidin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + cyanidin 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside)
-
-
-
?
2.4.2.51
UDP-D-xylose + delphinidin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + delphinidin 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside)
-
-
-
?
2.4.2.51
UDP-D-xylose + kaempferol 3-O-alpha-L-rhamnosyl-(1->6)-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + kaempferol 3-O-(beta-D-xylosyl-(1->2)[alpha-L-rhamnosyl-(1->6)]-beta-D-glucoside)
-
-
-
?
2.4.2.51
UDP-D-xylose + kaempferol 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + kaempferol 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside)
-
-
-
?
2.4.2.51
UDP-D-xylose + pelargonidin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + pelargonidin 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside)
-
-
-
?
2.4.2.51
UDP-D-xylose + quercetin 3-O-beta-D-glucoside
-
723428
Arabidopsis thaliana
UDP + quercetin 3-O-(beta-D-xylosyl-(1->2)-beta-D-glucoside)
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.51
30
-
assay at
Arabidopsis thaliana
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.51
6.5
-
-
Arabidopsis thaliana
2.4.2.51
7.5
-
assay at
Arabidopsis thaliana
General Information
EC Number
General Information
Commentary
Organism
2.4.2.51
evolution
flavonoid UGT phylogenetic tree, UGT79B1 belongs to a cluster in which UGTs transfer a glycosyl group to a sugar moiety of flavonoid glycosides
Arabidopsis thaliana
2.4.2.51
malfunction
anthocyanin is drastically reduced in ugt79b1 knockout mutants
Arabidopsis thaliana
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.4.2.51
evolution
flavonoid UGT phylogenetic tree, UGT79B1 belongs to a cluster in which UGTs transfer a glycosyl group to a sugar moiety of flavonoid glycosides
Arabidopsis thaliana
2.4.2.51
malfunction
anthocyanin is drastically reduced in ugt79b1 knockout mutants
Arabidopsis thaliana