Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dexter, R.; Qualley, A.; Kish, C.M.; Ma, C.J.; Koeduka, T.; Nagegowda, D.A.; Dudareva, N.; Pichersky, E.; Clark, D.
    Characterization of a petunia acetyltransferase involved in the biosynthesis of the floral volatile isoeugenol (2007), Plant J., 49, 265-75.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.84 complete coding region of PhCFAT cDNA subcloned into expression vector pET-28a and expressed in Escherichia coli Petunia x hybrida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.84 0.0226
-
 acetyl-CoA apparent KM value of recombinant protein with benzyl alcohol, pH 7.5, 20°C Petunia x hybrida
2.3.1.84 0.0275
-
 coniferyl alcohol apparent KM value of recombinant protein with acetyl-CoA, pH 7.5, 20°C Petunia x hybrida
2.3.1.84 0.0306
-
 acetyl-CoA apparent KM value of recombinant protein with coniferyl alcohol, pH 6.0, 20°C Petunia x hybrida
2.3.1.84 0.0441
-
 acetyl-CoA apparent KM value of recombinant protein with coniferyl alcohol, pH 7.5, 20°C Petunia x hybrida
2.3.1.84 0.0451
-
 acetyl-CoA apparent KM value of recombinant protein with 2-phenylethanol, pH 7.5, 20°C Petunia x hybrida
2.3.1.84 0.0565
-
 coniferyl alcohol apparent KM value of recombinant protein with acetyl-CoA, pH 6.0, 20°C Petunia x hybrida
2.3.1.84 3.6
-
 2-phenylethanol apparent KM value of recombinant protein with acetyl-CoA, pH 7.5, 20°C Petunia x hybrida
2.3.1.84 4.5
-
 benzyl alcohol apparent KM value of recombinant protein with acetyl-CoA, pH 7.5, 20°C Petunia x hybrida

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.84 Petunia x hybrida A1XWY7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.84 recombinant protein purified to 99% homogeneity using nickel-based affinity chromatography Petunia x hybrida

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.84 1-octanol + acetyl-CoA
-
 Petunia x hybrida acetyl-CoA + 1-octanol
-
 ?
2.3.1.84 2-phenylethanol + acetyl-CoA
-
 Petunia x hybrida 2-phenylethyl acetate + CoA
-
 ?
2.3.1.84 benzyl alcohol + acetyl-CoA
-
 Petunia x hybrida benzyl acetate + CoA
-
 ?
2.3.1.84 cinnamyl alcohol + acetyl-CoA
-
 Petunia x hybrida cinnamyl acetate + CoA
-
 ?
2.3.1.84 coniferyl alcohol + acetyl-CoA
-
 Petunia x hybrida coniferyl acetate + CoA
-
 ?
2.3.1.84 geraniol + acetyl-CoA
-
 Petunia x hybrida geranyl acetate + CoA
-
 ?
2.3.1.84 sinapyl alcohol + acetyl-CoA 71% of the activity with coniferyl alcohol Petunia x hybrida sinapyl acetate + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.84 PhCFAT
-
 Petunia x hybrida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.84 0.81
-
 coniferyl alcohol recombinant protein with acetyl-CoA, pH 7.5, 20°C Petunia x hybrida
2.3.1.84 0.82
-
 benzyl alcohol recombinant protein with acetyl-CoA, pH 7.5, 20°C Petunia x hybrida
2.3.1.84 0.91
-
 2-phenylethanol recombinant protein with acetyl-CoA, pH 7.5, 20°C Petunia x hybrida
2.3.1.84 0.93
-
 acetyl-CoA recombinant protein with 2-phenylethanol, pH 7.5, 20°C Petunia x hybrida
2.3.1.84 1.13
-
 acetyl-CoA recombinant protein with benzyl alcohol, pH 7.5, 20°C Petunia x hybrida
2.3.1.84 1.51
-
 acetyl-CoA recombinant protein with coniferyl alcohol, pH 7.5, 20°C Petunia x hybrida
2.3.1.84 2.05
-
 coniferyl alcohol recombinant protein with acetyl-CoA, pH 6.0, 20°C Petunia x hybrida
2.3.1.84 2.32
-
 acetyl-CoA recombinant protein with coniferyl alcohol, pH 6.0, 20°C Petunia x hybrida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.84 6
-
 highest activity with coniferyl alcohol Petunia x hybrida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.84 3.5 8.5
-
 Petunia x hybrida

Expression

EC Number Organism Comment Expression
2.3.1.84 Petunia x hybrida PhCFAT transcript levels decrease after ethylene release, diurnal rhythm of PhCFAT expression down