Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hall, D.; Yuan, X.X.; Murata, J.; De Luca, V.
    Molecular cloning and biochemical characterization of the UDP-glucose: flavonoid 3-O-beta-D-glucosyltransferase from Concord grape (Vitis labrusca) (2012), Phytochemistry, 74, 90-99.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.91 expressed in Escherichia coli DE3 pLys S cells Vitis labrusca
2.4.1.115 expressed in Escherichia coli DE3 pLysS cells Vitis labrusca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.91 0.00216
-
 quercetin in 100 mM Tris-HCl pH 8.0, at 30°C Vitis labrusca
2.4.1.91 0.00479
-
 cyanidin in 100 mM Tris-HCl pH 8.0, at 30°C Vitis labrusca
2.4.1.91 0.914
-
 UDP-alpha-D-glucose using cyanidin as cosubstrate, in 100 mM Tris-HCl pH 8.0, at 30°C Vitis labrusca
2.4.1.115 0.00216
-
 quercetin in 100 mM Tris-HCl pH 8.0, at 30°C Vitis labrusca
2.4.1.115 0.00479
-
 cyanidin in 100 mM Tris-HCl pH 8.0, at 30°C Vitis labrusca
2.4.1.115 0.914
-
 UDP-glucose in 100 mM Tris-HCl pH 8.0, at 30°C Vitis labrusca

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.115 50100
-
 x * 50100, calculated from amino acid sequence Vitis labrusca

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.91 Vitis labrusca A6N928 cultivar Concord
-
2.4.1.115 Vitis labrusca A6N928 cultivar Concord
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.91 glutathione Sepharose column chromatography Vitis labrusca
2.4.1.115 glutathione Sepharose column chromatography Vitis labrusca

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.91 exocarp pre-veraison exocarp Vitis labrusca
-
2.4.1.91 flower highest expression Vitis labrusca
-
2.4.1.91 flower bud
-
 Vitis labrusca
-
2.4.1.91 leaf
-
 Vitis labrusca
-
2.4.1.91 additional information not detected in mesocarp Vitis labrusca
-
2.4.1.115 exocarp pre-veraison exocarp Vitis labrusca
-
2.4.1.115 flower
-
 Vitis labrusca
-
2.4.1.115 flower bud
-
 Vitis labrusca
-
2.4.1.115 leaf
-
 Vitis labrusca
-
2.4.1.115 mesocarp
-
 Vitis labrusca
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.91 UDP-alpha-D-glucose + cyanidin this enzyme preferentially glucosylates cyanidin as compared with quercetin Vitis labrusca UDP + cyanidin 3-O-beta-D-glucoside
-
 ?
2.4.1.91 UDP-alpha-D-glucose + delphinidin
-
 Vitis labrusca UDP + delphinidin 3-O-beta-D-glucoside
-
 ?
2.4.1.91 UDP-alpha-D-glucose + dihydroquercetin weak substrate Vitis labrusca UDP + quercetin 3-O-beta-D-glucoside
-
 ?
2.4.1.91 UDP-alpha-D-glucose + isorhamnetin
-
 Vitis labrusca UDP + isorhamnetin 3-O-beta-D-glucoside
-
 ?
2.4.1.91 UDP-alpha-D-glucose + kaempferol weak substrate Vitis labrusca UDP + kaempferol 3-O-beta-D-glucoside
-
 ?
2.4.1.91 UDP-alpha-D-glucose + malvidin best substrate Vitis labrusca UDP + malvidin 3-O-beta-D-glucoside
-
 ?
2.4.1.91 UDP-alpha-D-glucose + peonidin
-
 Vitis labrusca UDP + peonidin 3-O-beta-D-glucoside
-
 ?
2.4.1.91 UDP-alpha-D-glucose + quercetin
-
 Vitis labrusca UDP + quercetin 3-O-beta-D-glucoside
-
 ?
2.4.1.115 additional information the enzyme is not able to further glucosylate cyanidin 3-O-beta-D-glucoside and it could not use UDP-galactose as a donor substrate Vitis labrusca ?
-
 ?
2.4.1.115 UDP-glucose + (2R,3R)-dihydroquercetin worst substrate Vitis labrusca UDP + (2R,3R)-dihydroquercetin 3-O-beta-D-glucoside
-
 ?
2.4.1.115 UDP-glucose + cyanidin
-
 Vitis labrusca UDP + cyanidin-3-O-beta-D-glucoside
-
 ?
2.4.1.115 UDP-glucose + delphinidin
-
 Vitis labrusca UDP + delphinidin-3-O-beta-D-glucoside
-
 ?
2.4.1.115 UDP-glucose + isorhamnetin
-
 Vitis labrusca UDP + isorhamnetin-3-O-beta-D-glucoside
-
 ?
2.4.1.115 UDP-glucose + kaempferol worst substrate Vitis labrusca UDP + kaempferol-3-O-beta-D-glucoside
-
 ?
2.4.1.115 UDP-glucose + malvidin best substrate Vitis labrusca UDP + malvidin-3-O-beta-D-glucoside
-
 ?
2.4.1.115 UDP-glucose + peonidin
-
 Vitis labrusca UDP + peonidin-3-O-beta-D-glucoside
-
 ?
2.4.1.115 UDP-glucose + quercetin while the recombinant enzyme preferentially glucosylates the 3 position of quercetin it is also able to glucosylate position 7 to a small extent in the early stages of the time course Vitis labrusca UDP + quercetin-3-O-beta-D-glucoside
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.115 ? x * 50100, calculated from amino acid sequence Vitis labrusca

Synonyms

EC Number Synonyms Comment Organism
2.4.1.91 UDP-glucose: flavonoid 3-O-glucosyltransferase
-
 Vitis labrusca
2.4.1.91 VL3GT
-
 Vitis labrusca
2.4.1.115 3GT
-
 Vitis labrusca
2.4.1.115 L3GT
-
 Vitis labrusca
2.4.1.115 UDP-glucose: flavonoid 3-O-glucosyltransferase
-
 Vitis labrusca

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.91 0.000113
-
 quercetin in 100 mM Tris-HCl pH 8.0, at 30°C Vitis labrusca
2.4.1.91 0.000247
-
 UDP-alpha-D-glucose using cyanidin as cosubstrate, in 100 mM Tris-HCl pH 8.0, at 30°C Vitis labrusca
2.4.1.91 0.000702
-
 cyanidin in 100 mM Tris-HCl pH 8.0, at 30°C Vitis labrusca
2.4.1.115 0.000113
-
 quercetin in 100 mM Tris-HCl pH 8.0, at 30°C Vitis labrusca
2.4.1.115 0.000247
-
 UDP-glucose in 100 mM Tris-HCl pH 8.0, at 30°C Vitis labrusca
2.4.1.115 0.000704
-
 cyanidin in 100 mM Tris-HCl pH 8.0, at 30°C Vitis labrusca

Expression

EC Number Organism Comment Expression
2.4.1.91 Vitis labrusca while there is little enzyme expression before veraison, these transcripts begin to accumulate at week 8 and 10 after flowering to reach maximal levels at week 12 after flowering and decreases thereafter up
2.4.1.115 Vitis labrusca gene expression is highest 12 weeks after flowering in the exocarp up

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.91 0.00027
-
 UDP-alpha-D-glucose using cyanidin as cosubstrate, in 100 mM Tris-HCl pH 8.0, at 30°C Vitis labrusca
2.4.1.91 0.052
-
 quercetin in 100 mM Tris-HCl pH 8.0, at 30°C Vitis labrusca
2.4.1.91 0.146
-
 cyanidin in 100 mM Tris-HCl pH 8.0, at 30°C Vitis labrusca
2.4.1.115 0.00027
-
 UDP-glucose in 100 mM Tris-HCl pH 8.0, at 30°C Vitis labrusca
2.4.1.115 0.052
-
 quercetin in 100 mM Tris-HCl pH 8.0, at 30°C Vitis labrusca
2.4.1.115 0.146
-
 cyanidin in 100 mM Tris-HCl pH 8.0, at 30°C Vitis labrusca