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Literature summary extracted from

  • Horakova, P.; Macickova-Cahova, H.; Pivonkova, H.; Spacek, J.; Havran, L.; Hocek, M.; Fojta, M.
    Tail-labelling of DNA probes using modified deoxynucleotide triphosphates and terminal deoxynucleotidyl transferase. Application in electrochemical DNA hybridization and protein-DNA binding assays (2011), Org. Biomol. Chem., 9, 1366-1371.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.7.31 analysis DNA tail-labelling using terminal deoxynucleotidyl transferase and modified deoxynucleoside triphosphates. Amino- and nitrophenyl-modified dNTPs are good substrates giving 3'-end stretches of different lengths depending on the nucleotide and concentration. d[7-Deaza-7-(3-nitrophenyl)]GTP is efficiently incorporated by the transferase to form long tail-labels at any oligonucleotide, resulting in a considerable enhancement of voltammetric signals due to the nitro group reduction. Discrimination between complementary and non-complementary target DNAs sequences by tail-labelled hybridization probes is easily accomplished, and tumour suppressor p53 protein is able to recognize a specific binding site within tail-labelled DNA substrates Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.31 Bos taurus P06526
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-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.31 commercial preparation
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 Bos taurus
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.31 d[5-(3-aminophenyl)]CTP + DNA 5-substituted dCTPs give products elongated by 1 to 5 nucleotides, with most intense bands corresponding to 2-3 extra nucleotides Bos taurus diphosphate + ?
-
 ?
2.7.7.31 d[5-(3-aminophenyl)]UTP + DNA 5-substituted dUTPs give products elongated by 4 to 5 nucleotides, the maximum length of the tail is about 14 nucleotides Bos taurus diphosphate + ?
-
 ?
2.7.7.31 d[5-(3-nitrophenyl)]CTP + DNA 5-substituted dCTPs give products elongated by 1 to 5 nucleotides, with most intense bands corresponding to 2-3 extra nucleotides Bos taurus diphosphate + ?
-
 ?
2.7.7.31 d[5-(3-nitrophenyl)]UTP + DNA 5-substituted dUTPs give products elongated by 4 to 5 nucleotides, the maximum length of the tail is about 14 nucleotides Bos taurus diphosphate + ?
-
 ?
2.7.7.31 d[7-deaza-7-(3-aminophenyl)]ATP + DNA the most intense band observed for reaction with 7-deaza,7-substituted dATPs corresponds to one nucleotide attached, but the products are rather polydisperse in lengths, reaching several tens of extra nucleotides Bos taurus diphosphate + ?
-
 ?
2.7.7.31 d[7-deaza-7-(3-aminophenyl)]GTP + DNA
-
 Bos taurus diphosphate + ?
-
 ?
2.7.7.31 d[7-deaza-7-(3-nitrophenyl)]ATP + DNA the most intense band observed for reaction with 7-deaza,7-substituted dATPs corresponds to one nucleotide attached, but the products are rather polydisperse in lengths, reaching several tens of extra nucleotides Bos taurus diphosphate + ?
-
 ?
2.7.7.31 d[7-deaza-7-(3-nitrophenyl)]GTP + DNA most useful substrate, efficiently incorporated by the transferase to form long tail-labels at any oligonucleotide Bos taurus diphosphate + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.31 TdT
-
 Bos taurus