Literature summary extracted from
Seo, Y.M.; Mathew, S.; Bea, H.S.; Khang, Y.H.; Lee, S.H.; Kim, B.G.; Yun, H.
Deracemization of unnatural amino acid: homoalanine using D-amino acid oxidase and omega-transaminase (2012), Org. Biomol. Chem., 10, 2482-2485.
Application
EC Number |
Application |
Comment |
Organism |
---|
2.6.1.18 |
biotechnology |
an alternative deracemization method for the efficient production of L-homoalanine using D-amino acid oxidase (vide infra) and omega-TA is proposed |
Vibrio fluvialis |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.6.1.18 |
expressed in Escherichia coli |
Vibrio fluvialis |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.6.1.18 |
benzaldehyde |
- |
Vibrio fluvialis |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.6.1.18 |
Vibrio fluvialis |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.6.1.18 |
using an Ni-NTA affinity column |
Vibrio fluvialis |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.6.1.18 |
2-oxobutyrate + benzylamine |
enzyme inhibition by benzaldehyde is the major hurdle in carrying out the omega-TA reaction efficiently. To overcome the product inhibition, a biphasic reaction system in which an organic solvent is employed to extract the inhibitory benzaldehyde from an aqueous system is used |
Vibrio fluvialis |
L-homoalanine + benzaldehyde |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.6.1.18 |
omega-TA |
- |
Vibrio fluvialis |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
2.6.1.18 |
37 |
- |
assay at |
Vibrio fluvialis |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
2.6.1.18 |
pyridoxal 5'-phosphate |
- |
Vibrio fluvialis |
|