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Literature summary extracted from

  • Dietrich, D.; van Belkum, M.J.; Vederas, J.C.
    Characterization of DcsC, a PLP-independent racemase involved in the biosynthesis of D-cycloserine (2012), Org. Biomol. Chem., 10, 2248-2254.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.1.19 expression in Escherichia coli Streptomyces lavendulae subsp. lavendulae

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.1.19 3-[[(carbamoylamino)oxy]methyl]oxiran-2-carboxylic acid irreversible inhibition Streptomyces lavendulae subsp. lavendulae
5.1.1.19 Hg2+ 0.01 mM, complete inhibition Streptomyces lavendulae subsp. lavendulae
5.1.1.19 iodoacetamide complete inhibition Streptomyces lavendulae subsp. lavendulae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.19 17
-
 O-ureido-D-serine pH 7.9, 30°C Streptomyces lavendulae subsp. lavendulae
5.1.1.19 110
-
 O-ureido-L-serine pH 7.9, 30°C Streptomyces lavendulae subsp. lavendulae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.1.19 31205
-
 x * 31205, ESI-TOF mass spectrometry Streptomyces lavendulae subsp. lavendulae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.1.19 O-ureido-L-serine Streptomyces lavendulae subsp. lavendulae the enzyme is involved in the biosynthesis of the antibiotic D-cycloserine O-ureido-D-serine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.19 Streptomyces lavendulae subsp. lavendulae D2Z026
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.19
-
 Streptomyces lavendulae subsp. lavendulae

Reaction

EC Number Reaction Comment Organism Reaction ID
5.1.1.19 O-ureido-L-serine = O-ureido-D-serine the enzyme employs a two-base mechanism, with a thiolate–thiol pair in the active site Streptomyces lavendulae subsp. lavendulae
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.19 O-ureido-L-serine
-
 Streptomyces lavendulae subsp. lavendulae O-ureido-D-serine
-
 r
5.1.1.19 O-ureido-L-serine the enzyme is involved in the biosynthesis of the antibiotic D-cycloserine Streptomyces lavendulae subsp. lavendulae O-ureido-D-serine
-
 r

Subunits

EC Number Subunits Comment Organism
5.1.1.19 ? x * 31205, ESI-TOF mass spectrometry Streptomyces lavendulae subsp. lavendulae

Synonyms

EC Number Synonyms Comment Organism
5.1.1.19 DcsC
-
 Streptomyces lavendulae subsp. lavendulae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.1.19 29
-
 O-ureido-D-serine pH 7.9, 30°C Streptomyces lavendulae subsp. lavendulae
5.1.1.19 158
-
 O-ureido-L-serine pH 7.9, 30°C Streptomyces lavendulae subsp. lavendulae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.1.19 7.8
-
-
 Streptomyces lavendulae subsp. lavendulae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.1.1.19 1.44
-
 O-ureido-L-serine pH 7.9, 30°C Streptomyces lavendulae subsp. lavendulae
5.1.1.19 1.71
-
 O-ureido-D-serine pH 7.9, 30°C Streptomyces lavendulae subsp. lavendulae