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Literature summary extracted from

  • Miyake, T.; Parsons, S.J.
    Functional interactions between choline kinase alpha, epidermal growth factor receptor and c-Src in breast cancer cell proliferation (2012), Oncogene, 31, 1431-1441.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.32 c-Src isoform CHKA2 total cellular activity and protein levels are regulated by c-Src Homo sapiens
2.7.1.32 epidermal growth factor receptor isoform CHKA2 total cellular activity and protein levels are regulated by epidermal growth factor receptor. In the presence of co-transfected epidermal growth factor receptor, the activity of wild type isoform CHKA2 is increased 2.5 fold Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.32 D306A inactive Homo sapiens
2.7.1.32 Y197/333F the mutant shows reduced activity in the presence of epidermal growth factor receptor compared to the wild type enzyme Homo sapiens
2.7.1.32 Y197F the mutant shows reduced activity in the presence of epidermal growth factor receptor compared to the wild type enzyme Homo sapiens
2.7.1.32 Y333F the mutant shows reduced activity in the presence of epidermal growth factor receptor compared to the wild type enzyme Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.32 Homo sapiens P35790
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.32 breast cancer cell
-
 Homo sapiens
-
2.7.1.32 HEK-293T cell
-
 Homo sapiens
-
2.7.1.32 MCF-10A cell
-
 Homo sapiens
-
2.7.1.32 MCF-7 cell
-
 Homo sapiens
-
2.7.1.32 MDA-MB-231 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.32 ATP + choline
-
 Homo sapiens ADP + phosphocholine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.32 Chka
-
 Homo sapiens
2.7.1.32 CHKA2 isoform Homo sapiens
2.7.1.32 choline kinase alpha
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.7.1.32 malfunction enzyme knockdown in MCF-7 and MCF-10A cells reduces epidermal growth factor-dependent cell proliferation Homo sapiens
2.7.1.32 physiological function the enzyme is required for maximum epidermal growth factor-dependent cell growth in mammary epithelium-derived cell lines MCF-7 and MCF-10A Homo sapiens