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Literature summary extracted from

  • Sun, R.; Eriksson, S.; Wang, L.
    Identification and characterization of mitochondrial factors modulating thymidine kinase 2 activity (2010), Nucleosides Nucleotides Nucleic Acids, 29, 382-385.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.21 3'-azido-2',3'-dideoxy-3,4-dihydrothymidine stimulates deoxycytidine phosphorylation catalyzed by thymidine kinase 2 Rattus norvegicus
2.7.1.21 3'-azido-3'-deoxythymidine stimulates deoxycytidine phosphorylation catalyzed by thymidine kinase 2 Homo sapiens
2.7.1.21 3'-deoxy-3'-fluorothymidine stimulates deoxycytidine phosphorylation catalyzed by thymidine kinase 2 Homo sapiens
2.7.1.21 3'-fluorothymidine stimulates deoxycytidine phosphorylation catalyzed by thymidine kinase 2 Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.21 expressed in Escherichia coli Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.21 3'-azido-2',3'-dideoxy-3,4-dihydrothymidine inhibits thymidine phosphorylation catalyzed by thymidine kinase 2 Homo sapiens
2.7.1.21 3'-azido-2',3'-dideoxy-3,4-dihydrothymidine inhibits thymidine phosphorylation catalyzed by thymidine kinase 2 Rattus norvegicus
2.7.1.21 3'-deoxy-3'-fluorothymidine inhibits thymidine phosphorylation catalyzed by thymidine kinase 2 Homo sapiens
2.7.1.21 3'-fluorothymidine inhibits thymidine phosphorylation catalyzed by thymidine kinase 2 Rattus norvegicus
2.7.1.21 additional information at physiological relevant concentration deoxycytidine phosphorylation is not inhibited by 3‘-azido-3'-deoxythmidine or 3'-fluorothymidine Homo sapiens
2.7.1.21 additional information at physiological relevant concentration deoxycytidine phosphorylation is not inhibited by 3'-azido-3'-deoxythmidine or 3'-fluorothymidine Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.21 0.0188
-
 thymidine pH and temperature not specified in the publication Rattus norvegicus
2.7.1.21 0.0414
-
 deoxycytidine pH and temperature not specified in the publication Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.21 mitochondrion
-
 Homo sapiens 5739
-
2.7.1.21 mitochondrion
-
 Rattus norvegicus 5739
-

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.21 Homo sapiens
-
-
-
2.7.1.21 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.21
-
 Homo sapiens
2.7.1.21 DEAE-Sephadex gel filtration Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.21 liver
-
 Homo sapiens
-
2.7.1.21 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.21 ATP + deoxycytidine
-
 Homo sapiens ADP + dCMP
-
 ?
2.7.1.21 ATP + deoxycytidine
-
 Rattus norvegicus ADP + dCMP
-
 ?
2.7.1.21 ATP + thymidine
-
 Homo sapiens ADP + dTMP
-
 ?
2.7.1.21 ATP + thymidine
-
 Rattus norvegicus ADP + dTMP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.21 thymidine kinase 2
-
 Homo sapiens
2.7.1.21 thymidine kinase 2
-
 Rattus norvegicus
2.7.1.21 TK2
-
 Homo sapiens
2.7.1.21 TK2
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.21 0.0014
-
 3'-deoxy-3'-fluorothymidine pH and temperature not specified in the publication Homo sapiens
2.7.1.21 0.0014
-
 3'-fluorothymidine pH and temperature not specified in the publication Rattus norvegicus
2.7.1.21 0.0039
-
 3'-azido-2',3'-dideoxy-3,4-dihydrothymidine pH and temperature not specified in the publication Homo sapiens
2.7.1.21 0.0039
-
 3'-azido-2',3'-dideoxy-3,4-dihydrothymidine pH and temperature not specified in the publication Rattus norvegicus

General Information

EC Number General Information Comment Organism
2.7.1.21 physiological function thymidine kinase 2 plays an important role in providing precursors for the replication and maintenance of mitochondrial DNA, especially in nonproliferating cells Homo sapiens
2.7.1.21 physiological function thymidine kinase 2 plays an important role in providing precursors for the replication and maintenance of mitochondrial DNA, especially in nonproliferating cells Rattus norvegicus