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Literature summary extracted from

  • Sugiarto, G.; Lau, K.; Li, Y.; Khedri, Z.; Yu, H.; Le, D.T.; Chen, X.
    Decreasing the sialidase activity of multifunctional Pasteurella multocida alpha2-3-sialyltransferase 1 (PmST1) by site-directed mutagenesis (2011), Mol. Biosyst., 7, 3021-3027.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.3.4 expressed in Escherichia coli BL21 (DE3) cells Pasteurella multocida

Protein Variants

EC Number Protein Variants Comment Organism
2.4.3.4 E271F the mutation does not affect alpha2-3-sialyltransferase activity Pasteurella multocida
2.4.3.4 E271F/R313Y the mutations do not affect alpha2-3-sialyltransferase activity Pasteurella multocida
2.4.3.4 R313Y the mutation does not affect alpha2-3-sialyltransferase activity Pasteurella multocida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.3.4 0.18
-
 CMP-N-acetylneuraminate mutant enzyme E271F, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.4 0.34
-
 CMP-N-acetylneuraminate mutant enzyme E271F/R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.4 0.44
-
 CMP-N-acetylneuraminate wild type enzyme, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.4 0.54
-
 4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside mutant enzyme E271F/R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.4 0.62
-
 CMP-N-acetylneuraminate mutant enzyme R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.4 0.67
-
 4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside mutant enzyme R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.4 0.71
-
 4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside mutant enzyme E271F, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.4 1.4
-
 4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside wild type enzyme, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.4 Pasteurella multocida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.3.4 Ni2+-NTA column chromatography Pasteurella multocida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.4 CMP-N-acetylneuraminate + 4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside best substrate, about 37% conversion Pasteurella multocida CMP + 4-methylumbelliferyl alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucoside
-
 ?
2.4.3.4 CMP-N-acetylneuraminate + lactose
-
 Pasteurella multocida CMP + NeuAcalpha(2->3)Galbeta(1->4)Glc
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.3.4 alpha2-3-sialyltransferase 1 multifunctional enzyme which has alpha2-6-sialyltransferase, alpha2-3-sialidase, and alpha2-3-trans-sialidase activities in addition to its major alpha2-3-sialyltransferase activity Pasteurella multocida
2.4.3.4 ST1
-
 Pasteurella multocida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.3.4 2 8 4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside mutant enzyme E271F, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.4 17
-
 4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside mutant enzyme E271F/R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.4 19
-
 CMP-N-acetylneuraminate mutant enzyme R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.4 19
-
 4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside mutant enzyme R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.4 23
-
 CMP-N-acetylneuraminate mutant enzyme E271F/R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.4 26
-
 CMP-N-acetylneuraminate mutant enzyme E271F, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.4 32
-
 CMP-N-acetylneuraminate wild type enzyme, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.4 47
-
 4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside wild type enzyme, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.3.4 6 10
-
 Pasteurella multocida

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.3.4 28
-
 4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside mutant enzyme R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.4 30
-
 CMP-N-acetylneuraminate mutant enzyme R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.4 32
-
 4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside mutant enzyme E271F/R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.4 34
-
 4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside wild type enzyme, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.4 39
-
 4-methylumbelliferyl 4-O-beta-D-galactosyl-beta-D-glucoside mutant enzyme E271F, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.4 69
-
 CMP-N-acetylneuraminate mutant enzyme E271F/R313Y, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.4 73
-
 CMP-N-acetylneuraminate wild type enzyme, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida
2.4.3.4 140
-
 CMP-N-acetylneuraminate mutant enzyme E271F, in Tris-HCl buffer (100 mM, pH 8.5), at 37°C Pasteurella multocida