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Literature summary extracted from

  • Filippini, S.; Solinas, M.M.; Breme, U.; Schlüter, M.B.; Gabellini, D.; Biamonti, G.; Colombo, A.L.; Garofano, L.
    Streptomyces peucetius daunorubicin biosynthesis gene, dnrF: sequence and heterologous expression (1995), Microbiology, 141, 1007-1016.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.180 heterologous expression of dnrF in Streptomyces liwidans TK23 and in Escherichia coli. Since the fusion protein does not show any hydroxylase activity, the dnrF gene is cloned into the pT7-7 vector that allows the expression of an unfused DnrF protein Streptomyces peucetius

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.180 52000
-
 x * 52000 Streptomyces peucetius

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.180 aklavinone + NADPH + H+ + O2 Streptomyces peucetius
-
 epsilon-rhodomycinone + NADP+ + H2O
-
 ?
1.14.13.180 aklavinone + NADPH + H+ + O2 Streptomyces peucetius ATCC 29050
-
 epsilon-rhodomycinone + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.180 Streptomyces peucetius P32009
-
-
1.14.13.180 Streptomyces peucetius ATCC 29050 P32009
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.180 aklavinone + NADPH + H+ + O2
-
 Streptomyces peucetius epsilon-rhodomycinone + NADP+ + H2O
-
 ?
1.14.13.180 aklavinone + NADPH + H+ + O2 modifications of the aklavinone molecule can influence the efficiency of the hydroxylation carried out by DnrF. While molecules modified in positions C-10 and C-13 are hydroxylated as efficiently as aklavinone (11-deoxycarminomycinone conversion), molecules 14-hydroxylated or 4-methylated are very poor substrates (11-deoxy-4-demethyladriamycinone and 11-deoxydaunomycinone conversion) Streptomyces peucetius epsilon-rhodomycinone + NADP+ + H2O
-
 ?
1.14.13.180 aklavinone + NADPH + H+ + O2
-
 Streptomyces peucetius ATCC 29050 epsilon-rhodomycinone + NADP+ + H2O
-
 ?
1.14.13.180 aklavinone + NADPH + H+ + O2 modifications of the aklavinone molecule can influence the efficiency of the hydroxylation carried out by DnrF. While molecules modified in positions C-10 and C-13 are hydroxylated as efficiently as aklavinone (11-deoxycarminomycinone conversion), molecules 14-hydroxylated or 4-methylated are very poor substrates (11-deoxy-4-demethyladriamycinone and 11-deoxydaunomycinone conversion) Streptomyces peucetius ATCC 29050 epsilon-rhodomycinone + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.180 ? x * 52000 Streptomyces peucetius

Synonyms

EC Number Synonyms Comment Organism
1.14.13.180 DnrF
-
 Streptomyces peucetius

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.180 37
-
 assay at Streptomyces peucetius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.180 7.7
-
 assay at Streptomyces peucetius

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.180 NADPH the DnrF enzyme contains two motifs that are common to a number of FAD- and NADPH-dependent enzymes. The enzyme catalyses the aklavinone 11-hydroxylation in a NADPH-dependent reaction Streptomyces peucetius