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Literature summary extracted from

  • Wolff, E.C.; Lee, S.B.; Park, M.H.
    Assay of deoxyhypusine synthase activity (2011), Methods Mol. Biol., 720, 195-205.
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.46 [eIF5A-precursor]-lysine + spermidine Homo sapiens
-
 [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.46 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.46 additional information development of a radioactive assay method for detection of spermidine incorporation into eIF5A, overview Homo sapiens ?
-
 ?
2.5.1.46 [eIF5A-precursor]-lysine + spermidine
-
 Homo sapiens [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
2.5.1.46 [eIF5A-precursor]-lysine + spermidine DHS catalyzes a NAD-dependent cleavage between N4 and C5 of spermidine and the transfer of the butylamine moiety to the ?-amino group of a specific lysine residue of the eIF5A precursor, eIF5A(Lys), to form the deoxyhypusine residue. The enzyme is modification is strictly specific for one single lysine residue of the cellular protein eukaryotic initiation factor 5A (eIF5A) and does not work on short peptides Homo sapiens [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine 1,3-diaminopropane is released as a byproduct from spermidine cleavage ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.46 DHS
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.46 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.46 9.2
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.46 NAD+ required Homo sapiens

General Information

EC Number General Information Comment Organism
2.5.1.46 physiological function deoxyhypusine synthase catalyzes the synthesis of deoxyhypusine [N?-(4-aminobutyl)-lysine], the first step in the post-translational synthesis of an unusual amino acid hypusine [N?-(4-amino-2-hydroxybutyl)-lysine] Homo sapiens