EC Number | Application | Comment | Organism |
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2.6.1.48 | synthesis | production of 5-aminovalerate and glutarate in recombinant Escherichia coli. When the davAB genes encoding delta-aminovaleramidase and lysine 2-monooxygenase, respectively, are introduced into a recombinant Escherichia coli strain allowing enhanced L-lysine synthesis, 0.27 and 0.5 g/l of 5-aminovalerate are produced directly from glucose by batch and fed-batch cultures, respectively. Further conversion of 5-aminovalerate into glutarate can be demonstrated by expression of the Pseudomonas putida gabTD genes encoding 5-aminovalerate aminotransferase and glutarate semialdehyde dehydrogenase. A recombinant Eschrerichia coli strain expressing the davAB and gabTD genes cultured in a medium containing 20 g/l glucose,10 g/l L-lysine and 10 g/l alpha-ketoglutarate, produces 1.7 g/l of glutarate | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
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2.6.1.48 | Pseudomonas putida | - |
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